1. A precipitate was formed at the oral opening, excretory pore and anal opening of the fourth stage juveniles of O. columbianum in the presence of immune serum in vitro, causing their immobilisation and subsequent death in large numbers. The precipitates failed to appear on these juveniles in normal serum or in saline; nor did they appear on the third stage juveniles on incubation in immune sera. Apparently the excretory-secretory products of the fourth stage juveniles of O. columbianum serve as antigen and play an important role in the stimulation of antibodies in the host.
2. Inactivation of the immune serum did not destroy the precipitin type of antibody, nor was it affected when the immune animals had been treated with cortisone.
3. Immune serum from animals receiving multiple infections showed a higher antibody titre.
4. The possible way in which the antibody acts on the fourth stage juveniles, causing their immobilisation and subsequent death, is discussed.