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The In Vitro Action of Immune Serum on the Juveniles of the Nematode, Oesophagostomum columbianum (Curtice, 1890) Stossich, 1899

Published online by Cambridge University Press:  05 June 2009

D. N. Dhar  and
Kunwar Suresh Singh
D. N. Dhar
Affiliation:
Post Graduate College of Animal Sciences, Indian Veterinary Research Institute, Izatnagar. U.P.
Kunwar Suresh Singh
Affiliation:
Post Graduate College of Animal Sciences, Indian Veterinary Research Institute, Izatnagar. U.P.
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Extract

1. A precipitate was formed at the oral opening, excretory pore and anal opening of the fourth stage juveniles of O. columbianum in the presence of immune serum in vitro, causing their immobilisation and subsequent death in large numbers. The precipitates failed to appear on these juveniles in normal serum or in saline; nor did they appear on the third stage juveniles on incubation in immune sera. Apparently the excretory-secretory products of the fourth stage juveniles of O. columbianum serve as antigen and play an important role in the stimulation of antibodies in the host.

2. Inactivation of the immune serum did not destroy the precipitin type of antibody, nor was it affected when the immune animals had been treated with cortisone.

3. Immune serum from animals receiving multiple infections showed a higher antibody titre.

4. The possible way in which the antibody acts on the fourth stage juveniles, causing their immobilisation and subsequent death, is discussed.

Type
Research Article
Information
Journal of Helminthology , Volume 42 , Issue 3-4 , September 1968 , pp. 269 - 278
Copyright
Copyright © Cambridge University Press 1968

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