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Sequence variability in four mitochondrial genes among Bunostomum trigonocephalum isolates from four provinces in China

Published online by Cambridge University Press:  15 October 2012

C.R. Wang ,
J.F. Gao ,
Q.C. Chang ,
F.C. Zou ,
Q. Zhao  and
X.Q. Zhu
C.R. Wang
Affiliation:
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province130118, PR China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
J.F. Gao
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province161006, PR China
Q.C. Chang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
F.C. Zou
Affiliation:
College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province650201, PR China
Q. Zhao*
Affiliation:
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province130118, PR China
X.Q. Zhu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province650201, PR China State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
*
*Corresponding authors:Fax: +86 (431) 84530026 E-mail: [email protected]; [email protected]
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Abstract

The present study examined sequence variability in four mitochondrial genes, namely cytochrome c oxidase subunit (cox1), cytochrome b (cytb) and NADH dehydrogenase subunits 1 and 5 (nad1 and nad5), among Bunostomum trigonocephalum isolates from four different geographic regions in China. Ten B. trigonocephalum samples were collected from each of the four provinces (Heilongjiang, Jilin, Shaanxi and Yunnan), China. A part of the cox1 (pcox1), cytb (pcytb), nad1 and nad5 genes (pnad1 and pnad5) were amplified separately from individual hookworms by polymerase chain reaction (PCR) and were subjected to direct sequencing in order to define sequence variations and their phylogenetic relationships. The intra-specific sequence variations within B. trigonocephalum were 0–1.9% for pcox1, 0–2.0% for pcytb, 0–1.6% for pnad1 and 0–1.7% for pnad5. The A+T contents of the sequences were 69.6–70.4% (pcox1), 71.9–72.7 (pcytb), 70.4–71.1% (pnad1) and 72.0–72.6% (pnad5). However, the inter-specific sequence differences among members of the family Ancylostomatidae were significantly higher, being 12.1–14.2% for pcox1, 13.7–16.0 for cytb, 17.6–19.4 for nad1 and 16.0–21.6 for nad5. Phylogenetic analyses based on the combined partial sequences of cox1, cytb, nad1 and nad5 using three inference methods, namely Bayesian inference (Bayes), maximum likelihood (ML) and maximum parsimony (MP), revealed that all the B. trigonocephalum samples form monophyletic groups, but samples from the same geographical origin did not always cluster together, suggesting that there was no obvious geographical distinction within B. trigonocephalum based on sequences of the four mtDNA genes. These results demonstrated the existence of low-level intra-specific variation in mitochondrial DNA (mtDNA) sequences among B. trigonocephalum isolates from different geographic regions.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 4 , December 2013 , pp. 416 - 421
Copyright
Copyright © Cambridge University Press 2012 

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References

Alemu, A., Atnafu, A., Addis, Z., Shiferaw, Y., Teklu, T., Mathewos, B., Birhan, W., Gebretsadik, S. & Gelaw, B. (2011) Soil transmitted helminths and Schistosoma mansoni infections among school children in Zarima town, northwest Ethiopia. BMC Infectious Diseases 11, 189.CrossRefGoogle ScholarPubMed
Borgsteede, F.H., Tibben, J., Cornelissen, J.B., Agneessens, J. & Gaasenbeek, C.P. (2000) Nematode parasites of adult dairy cattle in the Netherlands. Veterinary Parasitology 89, 287–296.CrossRefGoogle ScholarPubMed
Brooker, S., Bethony, J. & Hotez, P.J. (2004) Human hookworm infection in the 21st century. Advances in Parasitology 58, 197–288.CrossRefGoogle Scholar
Cerutti, M.C., Citterio, C.V., Bazzocchi, C., Epis, S., D'Amelio, S., Ferrari, N. & Lanfranchi, P. (2010) Genetic variability of Haemonchus contortus (Nematoda: Trichostrongyloidea) in alpine ruminant host species. Journal of Helminthology 84, 276–283.CrossRefGoogle ScholarPubMed
Chilton, N.B., Gasser, R.B. & Beveridge, I. (1995) Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). International Journal for Parasitology 25, 647–651.CrossRefGoogle Scholar
Dai, R.S., Liu, G.H., Song, H.Q., Lin, R.Q., Yuan, Z.G., Li, M.W., Huang, S.Y., Liu, W. & Zhu, X.Q. (2012) Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology 86, 245–251.CrossRefGoogle ScholarPubMed
Hotez, P. (2008) Hookworm and poverty. Annals of the New York Academy of Sciences 1136, 38–44.CrossRefGoogle ScholarPubMed
Hu, M., D'Amelio, S., Zhu, X., Paggi, L. & Gasser, R. (2001) Mutation scanning for sequence variation in three mitochondrial DNA regions for members of the Contracaecum osculatum (Nematoda: Ascaridoidea) complex. Electrophoresis 22, 1069–1075.3.0.CO;2-T>CrossRefGoogle ScholarPubMed
Li, M.W., Lin, R.Q., Song, H.Q., Sani, R.A., Wu, X.Y. & Zhu, X.Q. (2008) Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance. Electrophoresis 29, 2912–2917.CrossRefGoogle ScholarPubMed
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 1451–1452.CrossRefGoogle ScholarPubMed
Liu, G.H., Li, B., Li, J.Y., Song, H.Q., Lin, R.Q., Cai, X.Q., Zou, F.C., Yan, H.K., Yuan, Z.G., Zhou, D.H. & Zhu, X.Q. (2012a) Genetic variation among Clonorchis sinensis isolates from different geographic regions in China revealed by sequence analyses of four mitochondrial genes Journal of Helminthology 86, 479–484.CrossRefGoogle Scholar
Liu, G.H., Gasser, R.B., Su, A., Nejsum, P., Peng, L., Lin, R.Q., Li, M.W., Xu, M.J. & Zhu, X.Q. (2012b) Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets. PLoS Neglected Tropical Diseases 6, e1539.CrossRefGoogle ScholarPubMed
Makovcová, K., Langrová, I., Vadlejch, J., Jankovská, I., Lytvynets, A. & Borkovcová, M. (2008) Linear distribution of nematodes in the gastrointestinal tract of tracer lambs. Parasitology Research 104, 123–126.CrossRefGoogle ScholarPubMed
Page, R.D. (1996) TREEVIEW: an application to display phylogenetictrees on personal computers. Computer Applications in the Biosciences 12, 357–358.Google Scholar
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle ScholarPubMed
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.CrossRefGoogle ScholarPubMed
Strimmer, K. & Haeseler, A.V. (1996) Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13, 964–969.CrossRefGoogle Scholar
Swofford, D.L. (2002) PAUP*: Phylogenetic analysis using parsimony (and other methods). Sunderland, Massachusetts, Sinauer Associates.Google Scholar
Tariq, K.A., Chishti, M.Z. & Ahmad, F. (2010) Gastro-intestinal nematode infections in goats relative to season, host sex and age from the Kashmir valley, India. Journal of Helminthology 84, 93–97.CrossRefGoogle Scholar
Taylor, M.A., Coop, R.L. & Wall, R.L. (2007) Veterinary parasitology. 3rd edn. pp. 170–171. Oxford, UK, Blackwell Publishing.Google Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Wang, C.R., Qiu, J.H., Zhu, X.Q., Han, X.H., Ni, H.B., Zhao, J.P., Zhou, Q.M., Zhang, H.W. & Lun, Z.R. (2006) Survey of helminths in adult sheep in Heilongjiang Province People's Republic of China. Veterinary Parasitology 140, 378–382.CrossRefGoogle ScholarPubMed
Wang, C.R., Gao, J.F., Zhu, X.Q. & Zhao, Q. (2012) Characterization of Bunostomum trigonocephalum and Bunostomum phlebotomum from sheep and cattle by internal transcribed spacers of nuclear ribosomal DNA. Research in Veterinary Science 92, 99–102.CrossRefGoogle ScholarPubMed
Zhao, G.H., Mo, X.H., Zou, F.C., Weng, Y.B., Lin, R.Q., Xia, C.M. & Zhu, X.Q. (2009) Genetic variability among Schistosoma japonicum isolates from different endemic regions in China revealed by sequences of three mitochondrial DNA genes. Veterinary Parasitology 162, 67–74.CrossRefGoogle Scholar