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Identification and pathogenicity of Pratylenchus scribneri on tomato in Sichuan Province of People's Republic of China

Published online by Cambridge University Press:  10 January 2022

Y.H. Xia ,
J. Li ,
M.R. Sun ,
B. Lei ,
H.L. Li ,
Y. Li Open the ORCID record for Y. Li [Opens in a new window]  and
K. Wang
Y.H. Xia
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
J. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
M.R. Sun
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
B. Lei
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
H.L. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
Y. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
K. Wang
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
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Abstract

Root-lesion nematodes (Pratylenchus spp.) are a group of economically important pathogens that have caused serious economic losses in many crops. In 2019, root-lesion nematodes were recovered from tomato (Solanum lycopersicum) root samples collected from Sichuan Province, People's Republic of China (PRC). Extracted nematodes were disinfected, and one individual female was cultured on a carrot disc for propagation at 25 °C by parthenogenesis and designated the SC isolate. Afterwards, the isolate was identified on the basis of morphometric and molecular markers. Both morphometric characters and molecular analysis of the internal transcribed spacer region gene (ITS) of ribosomal DNA, the D2-D3 expansion region of the 28S rDNA gene and the mitochondrial cytochrome oxidase I (mtDNA-COI) gene revealed that the species of root-lesion nematode was Pratylenchus scribneri. The Bayesian tree inferred from the ITS rDNA, 28S rDNA and mtDNA-COI gene sequences also showed that this isolate formed a highly supported clade with other P. scribneri isolates. The pathogenicity of the root-lesion nematode SC isolate on tomato was assessed, showing that tomato was a suitable host for P. scribneri. To the best of our knowledge, this is the first report of P. scribneri on tomato in Sichuan Province, PRC. These are also the first molecular data obtained from P. scribneri on tomato in the PRC, and the pathogenicity of P. scribneri to tomato was studied for the first time. This study provides scientific data for the detection, identification and control of tomato root-lesion nematode disease.

Keywords

IdentificationpathogenicityPratylenchus scribneritomatoroot-lesion nematode
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e5
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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