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Frequency of Toxocara canis antibodies in Mexican paediatric patients with epilepsy

Published online by Cambridge University Press:  23 September 2019

M.d.L. Caballero-García ,
J. Simón-Salvador ,
J.C. Hernández-Aguilar ,
A. Reyes-Lopez ,
B. Nogueda-Torres  and
E. Jiménez-Cardoso Open the ORCID record for E. Jiménez-Cardoso [Opens in a new window]
M.d.L. Caballero-García
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
J. Simón-Salvador
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
J.C. Hernández-Aguilar
Affiliation:
Departamento de Neurología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Mexico, Mexico
A. Reyes-Lopez
Affiliation:
Medicina Basada en Evidencias, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Mexico, Mexico
B. Nogueda-Torres
Affiliation:
Departamento de Parasitología, Escuela Nacional de Ciencias Biológica, Instituto Politécnico Nacional
E. Jiménez-Cardoso*
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
*
Author for correspondence: E. Jiménez-Cardoso, E-mail: [email protected]
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Abstract

The relationship between epilepsy and the presence of visceral larva migrans caused by Toxocara canis in Mexican children remains uncertain; however, this relationship needs to be elucidated because these parasite larvae can invade the human central nervous system. Accordingly, this study aimed to determine the frequency and specificity of anti-T. canis antibodies in the sera of children with epilepsy to determine the relationship between this parasite and epilepsy. The sera samples of 214 children were examined: 111 children diagnosed with epilepsy and 103 clinically healthy children without neurological disorders. In the sera of each group, the presence and specificity of anti-T. canis and anti-Ascaris lumbricoides antibodies, as well as the cross-reactivity between them, were assessed using enzyme-linked immunosorbent assay and Western blotting analysis. Among the children with epilepsy, 25.2% exhibited seropositivity to T. canis. Cross-reactivity against the A. lumbricoides antigen was present in 46.8% of the children with epilepsy, whereas 11.7% of the children with epilepsy and anti-T. canis antibodies did not exhibit cross-reactivity against this antigen. The Western blotting analysis of the sera from the children with epilepsy demonstrated the presence of T. canis proteins, with molecular weights of 24, 35, 55, 70, 120 and 210 kDa, and A lumbricoides proteins with molecular weights of 70, 80 and 110 kDa. Our results revealed the presence of anti-T. canis antibodies in the children with epilepsy; furthermore, cross-reactivity tests with A. lumbricoides showed the importance of the presence of anti-T. canis antibodies in revealing the relationship between this parasite and epilepsy in children.

Keywords

Toxocara canisepilepsyserologyantigensantibodies
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e89
Copyright
Copyright © Cambridge University Press 2019 

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