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First report of Caenorhabditis brenneri (Nematoda: Rhabditida) isolated from the cadaver of Philippinella moellendorffi (Stylommatophora: Ariophantidae), a terrestrial slug in the Philippines

Published online by Cambridge University Press:  04 August 2022

L.B. Dalan Open the ORCID record for L.B. Dalan [Opens in a new window] ,
M.A.B. Diano Open the ORCID record for M.A.B. Diano [Opens in a new window] ,
I. Tandingan De Ley Open the ORCID record for I. Tandingan De Ley [Opens in a new window]  and
N.H.N. Sumaya Open the ORCID record for N.H.N. Sumaya [Opens in a new window]
L.B. Dalan
Affiliation:
Department of Biological Science, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, Andres Bonifacio, Tibanga, 9200 Iligan City, Philippines FBL-Nematology Research Group, Premier Research Institute of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines
M.A.B. Diano
Affiliation:
Department of Biological Science, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, Andres Bonifacio, Tibanga, 9200 Iligan City, Philippines FBL-Nematology Research Group, Premier Research Institute of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines
I. Tandingan De Ley
Affiliation:
Department of Nematology, University of California-Riverside, Riverside, California 92521, USA
N.H.N. Sumaya*
Affiliation:
Department of Biological Science, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, Andres Bonifacio, Tibanga, 9200 Iligan City, Philippines FBL-Nematology Research Group, Premier Research Institute of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines
*
Author for correspondence: N.H.N. Sumaya, E-mail: [email protected]
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Abstract

Gastropod-associated nematodes have been previously studied and documented worldwide, with some species forming host-specific association as obligate parasites of molluscs while others form intermediate and temporary association. Philippinella moellendorffi from Imelda, Zamboanga Sibugay, Philippines, were collected, washed and maintained in the laboratory until death. Cadavers were placed on nutrient agar to allow nematode proliferation. Nematode pure culture was obtained using one gravid female for propagation. Morphology and molecular analyses (18S ribosomal DNA (rDNA) and D2–D3 expansion segments of 28S rDNA) were employed as diagnostic tools in identifying the nematode species isolated from P. moellendorffi. The newly isolated nematode was identified as Caenorhabditis brenneri, thus designated as C. brenneri strain IZSP from the Philippines. This is the first record of C. brenneri isolated from the terrestrial slug P. moellendorffi.

Keywords

CaenorhabditisgastropodsPhilippinella18S rDNAD2–D3 expansion segments of 28S rDNA
Type
Short Communication
Information
Journal of Helminthology , Volume 96 , 2022 , e59
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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