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Comparative cytotoxicity of secondary hydatid cysts, protoscoleces, and in vitro developed microcysts of Echinococcus granulosus

Published online by Cambridge University Press:  05 June 2009

D. Janssen ,
A. Osuna ,
J. Lazuen  and
P. H. De Rycke
D. Janssen
Affiliation:
Laboratorium voor Zoöfysiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
A. Osuna
Affiliation:
Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
J. Lazuen
Affiliation:
Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
P. H. De Rycke
Affiliation:
Laboratorium voor Zoöfysiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
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Abstract

Infection with the metacestode of Echinococcus granulosus is characterized by a concomitant immunity. Survival of established and developing hydatid cysts in the intermediate host implies a mechanism to modulate its immunological reactions. In order to investigate this mechanism, secondary hydatid cysts were isolated from intraperitoneally infected laboratory white mice (strain NMRI) 12 months p.i. A number of hydatid cysts were freed from the surrounding host adventitial tissue. Monolayer cultures of non-stimulated peritoneal macrophages of NMRI mice were prepared and incubated in the presence of the hydatid cysts. By means of a trypan blue exclusion test and by measuring the incorporation of tritium labelled uridine, it was found that the presence of hydatid cysts reduced the viability of the macrophages in vitro. Toxic substances are probably secreted since the medium of cultured hydatid cysts also displayed cytotoxic activity. Hydatid cysts with adventitia, as well as culture medium of those cysts, were less toxic. When toxins, partially purified from hydatid cyst fluid, were previously incubated on a collagen coated surface, a reduced level of toxicity was found, suggesting that collagen of the host adventitia may play a role in controlling the liberation of toxins by the hydatid cyst. Virtually no toxicity was exerted by protoscoleces or by the medium of cultured protoscoleces, in contrast to in vitro vesiculated protoscoleces (so called microcysts). The results reveal a novel feature of hydatid cysts that may play a role in the survival of the parasite in the immunized host.

Keywords

Echinococcus granulosushydatidprotoscolexcytotoxicitycollagenCestoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 124 - 131
Copyright
Copyright © Cambridge University Press 1992

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