Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-28T15:15:25.983Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparative cytotoxicity of secondary hydatid cysts, protoscoleces, and in vitro developed microcysts of Echinococcus granulosus

Published online by Cambridge University Press:  05 June 2009

D. Janssen ,
A. Osuna ,
J. Lazuen  and
P. H. De Rycke
D. Janssen
Affiliation:
Laboratorium voor Zoöfysiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
A. Osuna
Affiliation:
Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
J. Lazuen
Affiliation:
Departamento de Parasitologia, Universidad de Granada, Campus Universitario Fuentenueva s/n, E-18071 Granada, Spain
P. H. De Rycke
Affiliation:
Laboratorium voor Zoöfysiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Get access Rights & Permissions [Opens in a new window]

Abstract

Infection with the metacestode of Echinococcus granulosus is characterized by a concomitant immunity. Survival of established and developing hydatid cysts in the intermediate host implies a mechanism to modulate its immunological reactions. In order to investigate this mechanism, secondary hydatid cysts were isolated from intraperitoneally infected laboratory white mice (strain NMRI) 12 months p.i. A number of hydatid cysts were freed from the surrounding host adventitial tissue. Monolayer cultures of non-stimulated peritoneal macrophages of NMRI mice were prepared and incubated in the presence of the hydatid cysts. By means of a trypan blue exclusion test and by measuring the incorporation of tritium labelled uridine, it was found that the presence of hydatid cysts reduced the viability of the macrophages in vitro. Toxic substances are probably secreted since the medium of cultured hydatid cysts also displayed cytotoxic activity. Hydatid cysts with adventitia, as well as culture medium of those cysts, were less toxic. When toxins, partially purified from hydatid cyst fluid, were previously incubated on a collagen coated surface, a reduced level of toxicity was found, suggesting that collagen of the host adventitia may play a role in controlling the liberation of toxins by the hydatid cyst. Virtually no toxicity was exerted by protoscoleces or by the medium of cultured protoscoleces, in contrast to in vitro vesiculated protoscoleces (so called microcysts). The results reveal a novel feature of hydatid cysts that may play a role in the survival of the parasite in the immunized host.

Keywords

Echinococcus granulosushydatidprotoscolexcytotoxicitycollagenCestoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 124 - 131
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Annen, J. M., Köhler, P. & Eckert, J. (1981) Cytotoxicity of Echinococus granulosus cyst fluid in vitro. Zeitschrift für Parasitenkunde, 65, 79–88.CrossRefGoogle Scholar
De Rycke, P. H. & Pennoit-De Cooman, E. (1973) Experimental secondary echinococcosis of Echinococcus granulosus. IV. Vaccination of host mice. Zeitschrift für Parasitenkunde, 42, 49–59.CrossRefGoogle ScholarPubMed
De Rycke, P. H. & Pennoit-De Cooman, E. (1978) Serial passages of larval Echinococcus granulosus from equine origin in mice. I. Infection from protoscoleces. Zeitschrift für Parasitenkunde, 55, 229–234.CrossRefGoogle Scholar
De Rycke, P. H., Janssen, D., Gamarro, F. & Osuna, A. (1985a) Lethal effect of Echinococcus granulosus cyst fluid on mouse macrophages. Biologisch Jaarboek Dodonaea, 53, 169–176.Google Scholar
De Rycke, P. H., Janssen, D., Gamarro, F., Osuna, A., Castanys, S. & Wéry, M. (1985b) Initial characterization of cytotoxic substances in Echinococcus granulosus hydatid cyst fluid. Proceedings of the 34th Annual Meeting of the American Society of Tropical Medicine and Hygiene(Nov. 1985,Miami), 153.Google Scholar
De Rycke, P. H., Janssen, D., Gamarro, F., Osuna, M. A & Carreras, M. A. (1989) Cytotoxicity and concomitant immunity in secondary echinococcosis of Echinococcus granulosus. Archivos Internacionales de la Hidatidosis, 29, 71.Google Scholar
De Rycke, P. H., Janssen, D., Osuna, A. & Lazuen, J. (1990) Immunohomeostatsis in hydatidosis (Echinococcus granulosus). In: Basic Research in Helminthiases (editors, Ehrlich, R., Nieto, A. & , Yarzábal) pp. 217–228. Ediciones Logos: Montevideo.Google Scholar
Dévé, F. (1931) Au sujet de la toxicité locale comparée de l'échinococcose hydatique et de l'échinococcose alvéolaire. Comptes Rendus des Séances de la Société de Biologie, 58, 687–688.Google Scholar
Janssen, D., Osuna, A., Gamarro, F., Carreras, M. A., Van Audenhove, K. & De Rycke, P. H. (1987) Functional role of the adventitial membrane in the defense of the intermediate host against Echinococcus granulosus. Proceedings of the Third Mediterranean Conference of Parasitology(Aug. 1987,Jerusalem), 53.Google Scholar
Janssen, D., De Rycke, P. H. & De Ridder, L. (1990) Development of larval Echinococcus granulosus confronted with chicken heart tissue in vitro. Parasitology, 100, 453–458.CrossRefGoogle ScholarPubMed
Kirkpatrick, C. J. & Svilenov, D. (1987) Studies on the adhesion of protoscoleces from Echinococcus multilocularis and E. granulosus to artificial substrates and endothelial cells in vitro. Parasitology Research, 74, 61–68.CrossRefGoogle Scholar
Klebe, R. J. (1974) Isolation of a collagen-dependent cell attachment factor. Nature 250, 248–251.CrossRefGoogle ScholarPubMed
Lloyd, S. & Soulsby, E. J. L. (1988) Immunological responses of the host. In: Parasitology in Focus (editor: Mehlborn, H.) pp. 619–650. Springer-Verlag: Berlin.CrossRefGoogle Scholar
Osuna, A., Gamarro, F., Castanys, S. & Ruiz-Perez, L. M. (1985) Effet des enzymes proteolytiques sur la pénétration du Trypanosoma cruzi dans les macrophages peritoneaux de souris. Annales de la Parasitologie Humaine et Comparée, 60, 383–388.CrossRefGoogle Scholar
Osuna, A., Gamarro, F., Janssen, D., Carreras, M. A., Castanys, S., Adroher, F. J. & De Rycke, P. H. (1987) Cytotoxicity of Echinococcus granulosus hydatid cyst fluid: species specific activity and biochemical characterization. Revista Iberica de Parasitologia, 47, 47–52.Google Scholar
Richards, K. S., Arme, C. & Bridges, J. F. (1983) Echinococcus granulosus equinus: an ultrastructural study of murine tissue response to hydatid cysts. Parasitology, 86, 407–417.CrossRefGoogle ScholarPubMed
Ronéus, O., Christensson, D. & Nilsson, N. G. (1982) The longevity of hydatid cysts in horses. Veterinary Parasitology, 11, 149–154.CrossRefGoogle ScholarPubMed
Schantz, P. M. (1977) Echinococcus granulosus, acute systemic allergic reactions to hydatid cyst fluid in infected sheep. Experimental Parasitology, 43, 268–285.CrossRefGoogle ScholarPubMed
Smyth, J. D. & Davies, Z. (1974) Occurrence of physiological strains of Echinococcus granulosus demonstrated by in vitro culture of protoscoleces from sheep and horse hydatid cysts. International Journal for Parasitology, 4, 443–445.CrossRefGoogle ScholarPubMed
Spruance, S. L. (1974) Latent period of 53 years in a case of hydatid cyst disease. Archives of Internal Medicine, 134, 741–742.CrossRefGoogle Scholar
Tabatabai, M., Ismaili, M. H., Nazarian, I. & Daneshbod, K. (1974) Pathophysiological changes resulting from intravenous injection of ovine hydatid cyst fluid to sheep. British Journal of Experimental Pathology, 55, 33–37.Google ScholarPubMed
Thompson, R. C. A. (1986) Biology and systematics of Echinococcus. In: Biology of Echinococcus and Hydatid Disease (editor, Thompson, R. C. A.) pp. 5–43. George Allen & Unwin: London.Google Scholar
Van Audenhove, K. (1986) Aspects of the cytotoxicity of Echinococcus granulosus against an experimental intermediate host (Mus musculus): specificity of the cytotoxin and the reaction of the host. Graduate thesis, University of Gent.Google Scholar
Wangoo, A., Ganguly, N. K. & Mahajan, R. C. (1989) Phagocytic functions of monocytes in murine model of Echinococcus granulosus of human origin. Indian Journal of Medical Research Section A–lnfectious Diseases, 89, 40–42.Google ScholarPubMed