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Studies on the ultrastructure and histochemistry of the lymph system in three species of amphistome (Trematoda: Digenea) Gigantocotyle explanatum, Gastrothylax crumenifer and Srivastavaia indica from the Indian Water Buffalo Bubalus bubalis

Published online by Cambridge University Press:  18 November 2009

T. S. Dunn ,
W. A. Nizami  and
R. E. B. Hanna
T. S. Dunn
Affiliation:
Department of Zoology, Queen's University, Belfast BT7 INN, N. Ireland
W. A. Nizami
Affiliation:
Department of Zoology, Aligarh Muslim University, Aligarh-202001, India
R. E. B. Hanna
Affiliation:
Department of Zoology, Queen's University, Belfast BT7 INN, N. Ireland
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Abstract

The lymph system of three amphistome parasites from buffaloes, Gigantocotyle explanatum, Gastrothylax crumenifer and Srivastavaia indica was studied using light microscope histochemistry and electron microscopy. In each case the system comprised a single pair of main longitudinal vessels which gave rise to numerous sub-dividing lateral branches. Although the finer lymph channels associated with most internal systems, they did not penetrate the basement membrane of any organ. The lymph vessels were delimited by a unit membrane and separated from adjacent cells by interstitial material. The lymph fluid consisted of an amorphous proteinaceous, lipid-rich matrix, containing naked nuclei and granules of various sizes. Complexes of endoplasmic reticulum were frequently associated with the nuclei. No distinct Golgi bodies or mitochondria were evident. The granules noted throughout the lymph morphologically resembled autophagosomes and lysosomes. Autophagy within the lymph system presumably mobilizes amino acids for subsequent transport to tissues undergoing active protein synthesis. The lymph channels displayed an intimate relationship with the general parenchyma. In particular, numerous protrusions of lymph occurred into the cytoplasm of certain specialized parenchymal cells surrounding the pharynx. Within these ‘juxtapharyńigeal’ cells autophagic degradation of sequestered lymph cytoplasm apparently occurred. In the three species of amphistome studied, the lymph system appears to function in storage and mobilization of amino acids and possibly lipids. It may also serve to distribute other small molecules throughout the body. The detection of haemoglobin in the lymph system of G. crumenifer and S. indica, but not in Gigantocotyle explanatum, suggests a further role in oxygen storage and transport.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 1 - 18
Copyright
Copyright © Cambridge University Press 1985

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