Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-27T14:27:51.725Z Has data issue: false hasContentIssue false
  • English
  • Français

Spermatological characteristics of Elstia stossichianum (Digenea, Mesometridae) from the intestine of the cow bream (Sarpa salpa) off Dakar, Senegal

Published online by Cambridge University Press:  16 October 2012

A.J.S. Bakhoum ,
P.I. Ndiaye ,
C.T. Bâ  and
J. Miquel
A.J.S. Bakhoum
Affiliation:
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s/n, E-08028Barcelona, Spain Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, E-08028Barcelona, Spain
P.I. Ndiaye
Affiliation:
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal
C.T. Bâ
Affiliation:
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal
J. Miquel*
Affiliation:
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s/n, E-08028Barcelona, Spain Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, E-08028Barcelona, Spain
*
*Fax: +34 93 402 45 04 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The current study was designed to increase the spermatological ultrastructural database on Digenea, thus contributing to the future establishment of phylogenetic relationships within this group based on ultrastructural characteristics of both spermiogenesis and spermatozoa. Spermiogenesis in Elstia stossichianum begins with the formation of a differentiation zone containing two centrioles, two striated rootlets, a nucleus, several mitochondria and an intercentriolar body constituted by seven electron-dense layers. Each centriole develops into a free flagellum growing orthogonally to the median cytoplasmic process. Proximo-distal fusion of the flagella with the median cytoplasmic process occurs after flagellar rotation. Both nucleus and mitochondria migrate before the complete proximo-distal fusion of both flagella with the median cytoplasmic process. Finally, the constriction of the ring of arched membranes gives rise to the young spermatozoon. The mature spermatozoon of E. stossichianum exhibits two axonemes of different length of the 9+‘1’ trepaxonematan pattern, a nucleus, a mitochondrion, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, a lateral expansion, spine-like bodies, cytoplasmic ornamented buttons and granules of glycogen. The formation of cytoplasmic ornamented buttons during the final stages of spermiogenesis is reported here for the first time.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 4 , December 2013 , pp. 422 - 431
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agostini, S., Miquel, J., Ndiaye, P.I. & Marchand, B. (2005) Dicrocoelium hospes Looss, 1907 (Digenea, Dicrocoeliidae): spermiogenesis, mature spermatozoon and ultrastructural comparative study. Parasitology Research 96, 38–48.CrossRefGoogle ScholarPubMed
Bâ, C.T. & Marchand, B. (1995) Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda. Mémoires du Muséum National d'Histoire Naturelle 166, 87–95.Google Scholar
Bakhoum, A.J.S., Bâ, C.T., Shimalov, V.V., Torres, J. & Miquel, J. (2011a) Spermatological characters of the digenean Rubenstrema exasperatum (Rudolphi, 1819) (Plagiorchioidea, Omphalometridae). Parasitology Research 108, 1283–1293.CrossRefGoogle ScholarPubMed
Bakhoum, A.J.S., Torres, J., Shimalov, V.V., Bâ, C.T. & Miquel, J. (2011b) Spermiogenesis and spermatozoon ultrastructure of Diplodiscus subclavatus (Pallas, 1760) (Paramphistomoidea, Diplodiscidae), an intestinal fluke of the pool frog Rana lessonae (Amphibia, Anura). Parasitology International 60, 64–74.CrossRefGoogle ScholarPubMed
Bakhoum, A.J.S., Ndiaye, P.I., Sène, A., Bâ, C.T. & Miquel, J. (2012) Spermiogenesis and ultrastructure of the spermatozoon of Wardula capitellata (Digenea, Mesometridae) an intestinal parasite of the sparid teleost Sarpa salpa in Senegal. Acta Parasitologica 57, 34–45.CrossRefGoogle ScholarPubMed
Bruňanská, M. (2010) Recent insights into spermatozoa development and ultrastructure in the Eucestoda. pp. 327–354in Lejeune, T. & Delvaux, P. (Eds) Human spermatozoa: maturation, capacitation and abnormalities. New York, NOVA Science Publishers.Google Scholar
Cribb, T.H., Bray, R.A., Littlewood, D.T.J., Pichelin, S.P. & Herniou, E.A. (2001) The Digenea. pp. 168–185in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of the Platyhelminthes. London, Taylor and Francis.Google Scholar
Ehlers, U. (1984) Phylogenetisches System der Plathelminthes. Verhandlungen des Naturwissenschaftlichen Vereins in Hamburg (NF) 27, 291–294.Google Scholar
Euzet, L., Świderski, Z. & Mokhtar-Maamouri, F. (1981) Ultrastructure comparée du spermatozoïde des Cestodes. Relations avec la phylogénèse. Annales de Parasitologie (Paris) 56, 247–259.Google Scholar
Foata, J., Quilichini, Y. & Marchand, B. (2007) Spermiogenesis and sperm ultrastructure of Deropristis inflata Molin, 1859 (Digenea, Deropristidae), a parasite of Anguilla anguilla. Parasitology Research 101, 843–852.CrossRefGoogle ScholarPubMed
Hendelberg, J. (1969) On the development of different types of spermatozoa from spermatids with two flagella in the Turbellaria with remarks on the ultrastructure of the flagella. Zoologiska Bidrag fran Uppsala 38, 1–50.Google Scholar
Iomini, C. & Justine, J.-L. (1997) Spermiogenesis and spermatozoon of Echinostoma caproni (Platyhelminthes, Digenea): transmission and scanning electron microscopy, and tubulin immunocytochemistry. Tissue & Cell 29, 107–118.CrossRefGoogle ScholarPubMed
Jones, A. & Blair, D. (2005) Family Mesometridae Poche, 1926. pp. 213–219in Jones, A., Bray, R.A. & Gibson, D.I. (Eds) Keys to the Trematoda. Wallingford, CABI Publishing.Google Scholar
Justine, J.-L. (1991) Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa. Canadian Journal of Zoology 69, 1421–1440.CrossRefGoogle Scholar
Justine, J.-L. (1995) Spermatozoal ultrastructure and phylogeny of the parasitic Platyhelminthes. Mémoires du Muséum National d'Histoire Naturelle 166, 55–86.Google Scholar
Justine, J.-L. (1998) Systématique des grands groupes de plathelminthes parasites: quoi de neuf? Bulletin de la Société Française de Parasitologie 16, 34–52.Google Scholar
Justine, J.-L. (2001) Spermatozoa as phylogenetic characters for the Platyhelminthes. pp. 231–238in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of the Platyhelminthes. London, Taylor and Francis.Google Scholar
Justine, J.-L. (2003) Ultrastructure des spermatozoïdes et phylogénie des Neodermata. pp. 359–380in Combes, C. & Jourdane, J. (Eds) Taxonomie, écologie et évolution des métazoaires parasites. Perpignan, Publications de l'Université de Perpignan.Google Scholar
Justine, J.-L. & Mattei, X. (1982) Réinvestigation de l'ultrastructure du spermatozoïde d'Haematoloechus (Trematoda: Haematoloechidae). Journal of Ultrastructure Research 81, 322–332.CrossRefGoogle ScholarPubMed
Levron, C., Ternengo, S. & Marchand, B. (2003) Ultrastructure of spermiogenesis and the spermatozoon of Helicometra fasciata (Digenea, Opecoelidae), a parasite of Labrus merula (Pisces, Teleostei). Acta Parasitologica 48, 255–264.Google Scholar
Levron, C., Ternengo, S. & Marchand, B. (2004) Ultrastructure of spermiogenesis and the spermatozoon of Monorchis parvus Looss, 1902 (Digenea, Monorchiidae), a parasite of Diplodus annularis (Pisces, Teleostei). Parasitology Research 93, 102–110.CrossRefGoogle ScholarPubMed
Levron, C., Miquel, J., Oros, M. & Scholz, T. (2010) Spermatozoa of tapeworms (Platyhelminthes, Eucestoda): advances in ultrastructural and phylogenetic studies. Biological Reviews 85, 523–543.CrossRefGoogle ScholarPubMed
Miquel, J., Fournier-Chambrillon, C., Fournier, P. & Torres, J. (2006) Spermiogenesis and spermatozoon ultrastructure of the cranial digenean Troglotrema acutum (Leuckart, 1842). Journal of Parasitology 92, 441–453.CrossRefGoogle ScholarPubMed
Ndiaye, P.I., Miquel, J., Fons, R. & Marchand, B. (2003) Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica L., 1758 (Digenea, Fasciolidae): scanning and transmission electron microscopy, and tubulin immunocytochemistry. Acta Parasitologica 48, 182–194.Google Scholar
Ndiaye, P.I., Miquel, J., Bâ, C.T. & Marchand, B. (2004) Spermiogenesis and ultrastructure of the spermatozoon of the liver fluke Fasciola gigantica Cobbold, 1856 (Digenea, Fasciolidae), a parasite of cattle in Senegal. Journal of Parasitology 90, 30–40.CrossRefGoogle ScholarPubMed
Ndiaye, P.I., Quilichini, Y., Sène, A., Bâ, C.T. & Marchand, B. (2011) Ultrastructure of the spermatozoon of the digenean Cricocephalus albus (Kuhl & van Hasselt, 1822) Looss, 1899 (Platyhelminthes, Pronocephaloidea, Pronocephalidae), parasite of ‘the hawksbill sea turtle’ Eretmochelys imbricata (Linnaeus, 1766) in Senegal. Zoologischer Anzeiger 250, 215–222.CrossRefGoogle Scholar
Pérez-del Olmo, A., Gibson, D.I., Fernández, M., Sanisidro, O., Raga, J.A. & Kostadinova, A. (2006) Descriptions of Wardula bartolii n. sp. (Digenea: Mesometridae) and three newly recorded accidental parasites of Boops boops L. (Sparidae) in the NE Atlantic. Systematic Parasitology 63, 99–109.Google Scholar
Quilichini, Y., Foata, J. & Marchand, B. (2007a) Ultrastructural study of the spermatozoon of Nicolla testiobliquum (Digenea, Opecoelidae) parasite of brown trout Salmo trutta (Pisces, Teleostei). Parasitology Research 101, 1295–1301.CrossRefGoogle ScholarPubMed
Quilichini, Y., Foata, J. & Marchand, B. (2007b) Ultrastructural study of the spermatozoon of Pronoprymna ventricosa (Digenea, Baccigerinae), parasite of the twaite shad Alosa fallax Lacepede (Pisces, Teleostei). Parasitology Research 101, 1125–1130.CrossRefGoogle ScholarPubMed
Quilichini, Y., Foata, J., Orsini, A. & Marchand, B. (2007c) Spermiogenesis and spermatozoon ultrastructure of Nicolla wisniewskii (Digenea: Opecoelidae), an intestinal parasite of brown trout Salmo trutta (Pisces: Teleostei). Journal of Parasitology 93, 469–478.CrossRefGoogle ScholarPubMed
Quilichini, Y., Foata, J., Justine, J.-L., Bray, R.A. & Marchand, B. (2010a) Spermatozoon ultrastructure of Aponurus laguncula (Digenea: Lecithasteridae), a parasite of Aluterus monoceros (Pisces, Teleostei). Parasitology International 59, 22–28.CrossRefGoogle ScholarPubMed
Quilichini, Y., Foata, J., Justine, J.-L., Bray, R.A. & Marchand, B. (2010b) Ultrastructural study of the spermatozoon of Heterolebes maculosus (Digenea, Opistholebetidae), a parasite of the porcupinefish Diodon hystrix (Pisces, Teleostei). Parasitology International 59, 427–434.CrossRefGoogle ScholarPubMed
Quilichini, Y., Foata, J., Justine, J.-L., Bray, R.A. & Marchand, B. (2011) Spermatozoon ultrastructure of Gyliauchen sp. (Digenea: Gyliauchenidae), an intestinal parasite of Siganus fuscescens (Pisces: Teleostei). Biological Bulletin 221, 197–205.CrossRefGoogle ScholarPubMed
Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17, 208–212.CrossRefGoogle ScholarPubMed
Seck, M.T., Marchand, B. & Bâ, C.T. (2007) Ultrastructure of spermiogenesis and the spermatozoon of Paramphistomum microbothrium (Fischoeder 1901; Digenea, Paramphistomidae), a parasite of Bos taurus in Senegal. Parasitology Research 101, 259–268.CrossRefGoogle ScholarPubMed
Seck, M.T., Marchand, B. & Bâ, C.T. (2008a) Spermiogenesis and sperm ultrastructure of Cotylophoron cotylophorum (Trematoda, Digenea, Paramphistomidae), a parasite of Bos taurus in Senegal. Parasitology Research 103, 157–166.CrossRefGoogle ScholarPubMed
Seck, M.T., Marchand, B. & Bâ, C.T. (2008b) Spermiogenesis and sperm ultrastructure of Carmyerius endopapillatus (Digenea, Gastrothylacidae), a parasite of Bos taurus in Senegal. Acta Parasitologica 53, 9–18.CrossRefGoogle Scholar
Świderski, Z. (1986) Three types of spermiogenesis in cestodes. pp. 2959–2960in Proceedings of the XIth International Congress of Electron Microscopy, Kyoto, Japan.Google Scholar
Thiéry, J.P. (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. Journal of Microscopy 6, 987–1018.Google Scholar