Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-16T08:47:09.934Z Has data issue: false hasContentIssue false
  • English
  • Français

Local and systemic antibody responses in gerbils following vaccination with irradiated or non-irradiated Trichostrongylus colubriformis larvae

Published online by Cambridge University Press:  05 June 2009

J. M. Maclean ,
M. Abebe ,
H. Wedrychowicz  and
P. H. Holmes
J. M. Maclean
Affiliation:
University of Glasgow Veterinary School, Glasgow, Scotland.
M. Abebe
Affiliation:
Institute of Pathobiology, Addis Ababa University, Ethiopia.
H. Wedrychowicz
Affiliation:
Department of Parasitology, Zoological Institute, University of Warszawa, Warsaw, Poland.
P. H. Holmes
Affiliation:
University of Glasgow Veterinary School, Glasgow, Scotland.
Get access Rights & Permissions [Opens in a new window]

Abstract

Groups of 10 Mongolian gerbils. Meriones unguiculatus, were vaccinated with 1,500 gamma-irradiated Trichostrongylus colubriformis infective larvae (L3) or with non-irradiated larvae. 25 days later five gerbils from each group were necropised and the remaining gerbils challenged with 1,500 non-irradiated T. colubriformis infective larvae. Systemic, local intestinal and coproantibody levels were compared in each group of gerbils 25 days after vaccination and 26 days after challenge. Strong local intestinal and faecal antibody responses were detected. Coproantibodies reflected antibody levels in the intestinal contents and in mucosal extracts. The results gave further support to the view that coproantibody measurements provide a sensitive index of immunity at mucosal surfaces to intestinal parasites.

Keywords

Trichostrongylus colubriformisMeriones unguiculatusantibody responsecoproantibodiesvaccination
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 4 , December 1986 , pp. 263 - 278
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Adams, D. B., Merrit, G. C. & Cripps, A. W. (1980) Intestinal lymph and the local antibody and immunoglobulin response to infection by Trichostrongylus colubriformis in sheep. Australian Journal of Experimental Biology and Medical Science, 58, 167–177.CrossRefGoogle ScholarPubMed
Burrows, W. & Havens, M. E. (1948) Studies on immunity to Asiatic cholera. V. The absorption of immune globulin from the bowel and its secretion in the urine and faeces of experimental animals and human volunteers. Journal of Infectious Diseases, 82, 235–250.CrossRefGoogle Scholar
Cripps, A. W. & Adams, D. B. (1978) Flow and portein composition of intestinal lymph in sheep infected with the enteric nematode Trichostrongylus colubriforms. Australian Journal of Experimental Biology and Medical Science, 56, 225–235.CrossRefGoogle Scholar
Dineen, J. K. & Wagland, B. M. (1982) Immunoregulation of parasites in natural host-parasite systems—with special reference to the gastrointestinal nematodes of sheep. In: Biology and the Control of Endoparasites (Editors Symons, L. E. A., Donald, A. D. and Dinee, J. K.) pp. 297–329, Academic Press: Sydney.Google Scholar
Freter, S., Mondal, S. P. DeA., Shrivastava, D. L. & Sunderman, F. W. Jr.(1965) Coproantibody and serum antibody in cholera patients. Journal of Infections Diseases, 115, 83–87.CrossRefGoogle ScholarPubMed
Gordon, H. McL., Mulligan, W. & Reinecke, R. K. (1960) Trichostrongylus colubriformis in the guinea pig: Studies with irradiated larvae. Australian Veterinary Journal, 36, 466–471.Google Scholar
Gordon, H. McL. & Whitlock, J. H. (1939) A new technique for counting eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research, Australia, 12, 50–52.Google Scholar
Gregg, P., Dineen, J. K. & Griffiths, D. (1976) The effect of γ-radiation on the development of infective larvae of Trichostrongylus colubriformis in guinea pigs and sheep. Veterinary Parasitology, 2, 363–375.Google Scholar
Herbert, W. J. (1975) Passive haemagglutination with special reference to the tanned cell technique. In: Handbook of Experimental Immunology, Vol. 1 (editor Weir, D. H.) pp. 20.1–20.20, Blackwell Scientific Publications: Oxford.Google Scholar
Herlich, H. (1966) Immunity to Trichostrongylus colubriformis in guinea pigs and lambs. Journal of Parasitlolgy, 52, 871–874.Google Scholar
Jenkins, E. M. & Roberts, W. (1980) Studies on coproantibody in swine dysentery. In: Proceedings of the International Pig Veterinary Society Congress, Copenhagen, Denmark.Google Scholar
Jennings, F. W., Mulligan, W. & Urquhart, G. M. (1963) Variables in X-ray ‘inactivation’ of Nippostrongylus brasiliensis larvae. Experimental Parasitology, 13, 367–373.Google Scholar
Kates, K. C. & Thompson, D. E. (1967) Acitivity of three anthelmintics against mixed infections of two Trichostrongylus species in gerbils, sheep and goats. Proceedings of the Helminthological Society of Washington, 34, 228–236.Google Scholar
Kates, K. C. & Thompson, D. E. (1968) Susceptibility of gerbils and young white rats to simultaneous infection with Trichostrongylus axei and T. colubriformis. Proceedings of the Helminthological Society of Washington, 35, 102–106.Google Scholar
Leyland, S. E. Jr. (1963) Studies in Trichostrongylus axei (Cobbold, 1879). VIII. Some quantitative aspects pf experimental infection of the Mongolian gerbil (Meriones unguiculatus). Journal of Parasitology, 49, 617–622.Google Scholar
Maclean, J. M. (1985) Some aspects of local immunity and pathogenesis in rodents infected with Nippostrongylus brasiliensis and Trichostrongylus colubriformis Ph.D. Thesis. University of Glasgow.Google Scholar
Mulligan, W., Gordon, H. McL., Stewart, D. F. & Wagland, B. M. (1961) The use of irradiated larvae as immunising agents in Haemonchus contortus and Trichostrongylus colubriformis infections of sheep. Australian Journal of Agricultural Research, 12, 1175–1187.CrossRefGoogle Scholar
Ogilvie, B. M. (1965) Role of adult worms in immunity of rats to Nippostrongylus brasiliensis. Parasitology, 55, 325–335.Google Scholar
Ostlind, D. A. & Cifelli, S. (1981) Efficacy of thiabendazole, levamisole hydrochloride and the major natural avermectins against Trichostrongylus colubriformis in the gerbil (Meriones unguiculatus). Research in Veterinary Science, 31, 255–256.Google Scholar
Panitz, E. & Shum, K. L. (1981a) Efficacy of four anthelmintics in Trichostrongylus axei or T. colubriformis infections in the gerbil, Meriones unguiculatus. Journal of Parasitology, 67, 135–136.CrossRefGoogle ScholarPubMed
Panitz, E. & Shum, K. L. (1981b) Distribution of Trichostrongylus axei, T. vitrinus and T. colubriformis in the stomach and small intestine of the gerbil Meriones unguiculatus. Journal of Parasitology, 67, 218–219.Google Scholar
Prochazka, Z. & Mulligan, W. (1965) Immunological studies on Nippostrongylus brasiliensis infection in the rat: experiments with irradiated larvae. Experimental Parasitology, 17, 51–56.CrossRefGoogle ScholarPubMed
Sinski, E. & Holmes, P. H. (1978) In vitro binding of IgG and Iga to Nippostrongylus brasiliensis measured by radio-immunoassay. Journal of Parasitology, 64, 189–191.Google Scholar
Wagland, B. M., Steel, J. W., Windon, R. G. & Dineen, J. K. (1984) The response of lambs to vaccination and challenge with Trichostrongylus colubriformis: Effects of plane of nutrition on, and the inter-relationship between, immunological unresponsiveness and resistance. International Journal for Parasitology, 14, 39–44.Google Scholar
Wedrychowicz, H., Maclean, J. M. & Holmes, P. H. (1983) The detection and measurement of coproantibodies to Nippostrongylus brasiliensis in rats following a primary infection. Parasite Immunology, 5, 277–287.Google Scholar
Wedrychowicz, H., Maclean, J. M. & Holmes, P. H. (1984a) The effects of Trypanosoma brucei infection on local and systemic antibody responses of rats to Nippostrongylus brasiliensis Tropenmedizin und Parasitologie, 34, 207–212.Google Scholar
Wedrychowicz, H., Maclean, J. M. & Holmes, P. H. (1984b) Secretory IgA responses in rats to antigens of various developmental stages of Nippostrongylus brasiliensis. Parasitology, 89, 145–157.CrossRefGoogle ScholarPubMed
Wedrychowicz, H., Maclean, J. M. & Holmes, P. H. (1984c) Nippostrongylus brasiliensis: Local humoral responses in the lungs and intestines of rats following vaccination with irradiated larvae. Experimental Parasitology, 57, 25–33.Google Scholar
Wedrychowicz, H., Maclean, J. M. & Holmes, P. H. (1985) Some observations on a possible role of lung and fecal IgA antibodies in immunity of rats to Nippostrongylus brasiliensis. Journal of Parasitology, 71, 62–69.Google Scholar
Williams, G. A. H. & Palmer, B. H. (1964) Trichostrongylus colubriformis (a nematode parasite of sheep and other ruminants): a test organism in screening for sheep anthelmintics in the laboratory. Nature (London), 203, 1399–1400.Google Scholar
Yokogawa, S. (1922) The development of Heligmosomum muris Yokagawa, a nematode from the intestine of the wild rat. Parasitology, 14, 127–166.CrossRefGoogle Scholar