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Immunogenic activity of the fish tapeworm Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) in BALB/c mice

Published online by Cambridge University Press:  03 December 2013

D.P.B.G. Mattos ,
M.A. Verícimo ,
L.M.S. Lopes  and
S.C. São Clemente
D.P.B.G. Mattos*
Affiliation:
Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340, Brazil
M.A. Verícimo
Affiliation:
Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Outeiro São João Batista, Centro, Niterói - RJ 24210-150, Brazil
L.M.S. Lopes
Affiliation:
Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 24230-340, Brazil
S.C. São Clemente
Affiliation:
Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340, Brazil
*
*E-mail: [email protected]
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Abstract

The aim of this study was to verify the immunogenicity of Pterobothrium heteracanthum (Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained from Micropogonias furnieri (Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 2 , March 2015 , pp. 203 - 207
Copyright
Copyright © Cambridge University Press 2013 

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