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Immunisation of baboons against Schistosoma mansoni using irradiated S. mansoni cercariae and schistosomula and non-irradiated S. rodhaini cercariae

Published online by Cambridge University Press:  05 June 2009

M. G. Taylor ,
E. R. James ,
G. S. Nelson ,
Q. Bickle ,
B. J. Andrews ,
A. R. Dobinson  and
G. Webbe
M. G. Taylor
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
E. R. James
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
G. S. Nelson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
Q. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
B. J. Andrews
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
A. R. Dobinson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
G. Webbe
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 HatfieldRoad, St. Albans, Herts.
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Abstract

In an attempt to develop a non-pathogenic procedure for immunising baboons against S. mansoni, groups of five baboons were exposed to three doses of 5000 6 Kr-irradiated S. mansoni cercariae or to similar numbers of normal S. rodhaini cercariae and challenged at week 15 with 500 normal S. mansoni cercariae. Faecal egg counts, worm and tissue egg counts, and histopathological examination, showed that neither of the immunising schedules had produced significant protection. In the second experiment baboons were injected by the intramuscular route with 31000 schistosomula of S. mansoni in three doses and the irradiation dose was reduced to near the minimum required for worm sterilisation (2·1–2·4 Kr). Challenge with 3500 normal cercariae of S. mansoni 21 weeks after the first immunising dose again showed no significant protection, although reductions of 20–30% were found, in egg and worm counts resulting from the challenge. These results indicate that it may be difficult to develop an effective live vaccine for S. mansoni unless the antigenicity of the immunising larvae can be greatly increased.

Type
Research Article
Information
Journal of Helminthology , Volume 50 , Issue 3 , September 1976 , pp. 215 - 221
Copyright
Copyright © Cambridge University Press 1976

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References

REFERENCES

Amin, M. A., Nelson, G. S. and Saoud, M. F. A. (1968) Studies on heterologous immunity in Schistosomiasis. II. Heterologous schistosome immunity in rhesus monkeys. Bulletin of the World Health Organization, 38, 19–27.Google Scholar
Bell, D. R. (1963) A new method for counting Schistosoma mansoni eggs in faeces, with special reference to chemotherapeutic trials. Bulletin of the World Health Organization, 29, 525–530.Google Scholar
Cheever, A. W., Erickson, D. G., Sadun, E. H. and Von Lichtenberg, F. (1974) Schistosoma japonicum infection in monkeys and baboons; parasitological and pathological findings. American Journal of Tropical Medicine & Hygiene, 23, 51–64.CrossRefGoogle ScholarPubMed
Clegg, J. A. and Smithers, S. R. (1972) The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. InternationalJournal of Parasitology, 2, 79–98.CrossRefGoogle ScholarPubMed
Damian, R. T.Green, N. D. and Fitzgerald, K. (1974) Schistosomiasis mansoni in baboons. II. Acquisition of immunity to challenge infection after repeated small exposures to cercariae of Schistosoma mansoni. American Journal of Tropical Medicine & Hygiene, 23, 78–80.CrossRefGoogle ScholarPubMed
Eveland, L. K., Hsu, S. Y. L. and Hsu, H. F. (1969) Cross-immunity of Schistosoma japonicum S. mansoni and S. bovis in rhesus monkeys. Journal of Parasitology, 55, 279–288.CrossRefGoogle ScholarPubMed
Hsu, S. Y. L. (1970) Immunisation against Schistosoma japonicum in chimpanzees by administration of X-irradiated cercariae. Transactions of the Royal Society of Tropical Medicine & Hygiene, 64, 597–600.CrossRefGoogle ScholarPubMed
Sadun, E. H., Bruce, J. I. and Macomber, P. B. (1964) Parasitologic, pathologic and serologic reactions to Schistosoma mansoni in monkeys exposed to irradiated cercariae. American Journal of Tropical Medicine & Hygiene, 13, 548–557.CrossRefGoogle ScholarPubMed
Sadun, E. H., Von Lichtenberg, F., Cheever, A. W. and Erickson, D. G. (1970a) Schistosomiasis mansoni in the chimpanzee. The natural history of chronic infections after single and multiple exposures. American Journal of Tropical Medicine & Hygiene, 19, 258–277.CrossRefGoogle ScholarPubMed
Sadun, E. H., Von Lichtenberg, F., Cheever, A. W., Erickson, D. G. and Hickman, R. L. (1970b) Experimental infection with Schistosoma haematobium in chimpanzees. American Journal of Tropical Medicine & Hygiene, 19, 427–458.CrossRefGoogle ScholarPubMed
Smithers, S. R. and Terry, R. J. (1965) Naturally acquired resistance to experimental injections of Schistosoma mansoni in the rhesus monkey. Parasitology, 55, 701–710.CrossRefGoogle Scholar
Taylor, M. G., Nelson, G. S., Smith, M., Andrews, B. J. (1973a) Studies on heterologous immunity in Schistosomiasis. 7. Observations on the development of acquired homologous and heterologous immunity to Schistosoma mansoni in baboons. Bulletin of the World Health Organization, 49, 57–65.Google ScholarPubMed
Taylor, M. G., Nelson, G. S., Smith, M. and Andrews, B. J. (1973b) Comparison of the infectivity and pathogenicity of six species of African Schistosomes and their hybrids. 2. Baboons. Journal of Helminthology, 47, 455–485.CrossRefGoogle ScholarPubMed
Taylor, M. G., James, E. R., Nelson, G. S., Bickle, Q., Dunne, D. and Webbe, G. (1976) Immunisation of sheep against Schistosoma mattheel using either irradiated cercariae or irradiated schistosomula. Journal of Helminthology, 50, 1–9.CrossRefGoogle ScholarPubMed
Warren, K. S. (1972) The immunopathogenesis of Schistosomiasis: a multi-disciplinary approach. Transactions of the Royal Society of Tropical Medicine & Hygiene, 66, 417–434.CrossRefGoogle Scholar
Webbe, G. and James, C. (1973) Acquired resistance to Schistosoma haematobium in the baboon (Papio anubls). Transactions of the Royal Society of Tropical Medicine & Hygiene, 67, 151–152.CrossRefGoogle Scholar