Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-09T16:24:45.112Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

Published online by Cambridge University Press:  29 June 2010

Lemu Golassa ,
Tamrat Abebe  and
Asrat Hailu
Lemu Golassa*
Affiliation:
Department of Medical Laboratory Sciences and Pathology, College of Public Health and Medical Sciences, Jimma University, PO Box 378, Jimma, Ethiopia
Tamrat Abebe
Affiliation:
Microbiology, Immunology and Parasitology Department, Medical Faculty, Addis Ababa University, PO Box 9086, Addis Ababa, Ethiopia
Asrat Hailu
Affiliation:
Microbiology, Immunology and Parasitology Department, Medical Faculty, Addis Ababa University, PO Box 9086, Addis Ababa, Ethiopia
*
*E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Echinococcosis is a zoonotic infection caused by adult or larval (metacestode) stages of cestodes belonging to the genus Echinococcus. The purpose of this study was to evaluate the antigenic ability of hydatid cyst fluid antigen for the diagnosis of hydatidosis in cattle using enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination test (IHA). The source of the antigens for the serological tests was fertile crude cyst fluids collected from naturally infected sheep at the Addis Ababa abattoir. A total of 502 sera were collected from 329 uninfected cattle and 173 hydatid-infected cattle which were confirmed by post-mortem examination. Most cysts were sterile and multiple organ infection predominated. Of 173 infected cattle, 166 (96.0%; confidence interval (CI) 91.8–98.4) were positive using ELISA while 7 (4.0%) were negative. Of 329 sera from uninfected cattle, 274 (83.3%; CI 78.8–87.2) were found to be negative and the remaining 55 (16.7%) were positive by ELISA. Of 173 infected cattle, 151 (87.3%; CI 81.4–91.9) were positive and 22 (12.7%) were negative by IHA. Of 329 negative sera tested using IHA, 266 (80.9%; CI 76.2–85.0) were negative and the remaining 63 (19.1%) were positive. The false positive and negative values of ELISA were 4.0 and 16.7%, respectively, and the corresponding values of IHA were 12.7 and 19.1%. The sensitivity and diagnostic efficiency of IHA were 87.2 and 83.6%, respectively. Crude hydatid cyst fluid antigen seems to have reasonable antigenic properties and hence could be employed for epidemiological surveillance of cattle hydatidosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 1 , March 2011 , pp. 100 - 108
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ali, A., Biluts, H. & Gulilat, D. (2005) Experience of surgical therapy in 72 patients with thoracic hydatidosis over a 10-year period. Ethiopian Medical Journal 43, 1–8.Google ScholarPubMed
Bakantz, S., Rein, R. & Rent, F. (1946) Studies in complement fixation. Preservation of sheep blood in citrate dextrose mixture (modified Alsever's solution) for use in complement fixation reaction. Journal of Laboratory and Clinical Medicine 31, 394–399.Google Scholar
Baldock, F., Arthur, J. & Lawrence, R. (1985) A meat works survey of bovine hydatidosis in southern Queensland. Australian Veterinary Journal 62, 238–242.CrossRefGoogle Scholar
Berhe, G. (2008) Abattoir survey on cattle hydatidosis in Tigray Region of Ethiopia. Tropical Animal Health and Production 41, 1347–1352.CrossRefGoogle Scholar
Blundell-Hasell, S. (1969) Serological diagnosis of larval cestodes in sheep. Australian Veterinary Journal 45, 334–336.CrossRefGoogle ScholarPubMed
Cabrera, P., Haran, G., Benavidez, U., Valledor, S., Perera, G., Lloyd, S., Gemmell, M., Baraibar, M., Morana, A., Maissonave, J. & Carballo, M. (1996) Transmission dynamics of Echinococcus granulosus, Taenia hydatigena and Taenia ovis in sheep in Uruguay. International Journal of Parasitology 25, 807–813.CrossRefGoogle Scholar
Craig, P. (1997) Immunodiagnosis of Echinococcus granulosus and a comparison of techniques for diagnosis of canine echinococcosis. pp. 85–118in Andersen, F., Ouhelli, H. & Kachani, M. (Eds) Compendium on cystic echinococcosis in Africa and in Middle Eastern countries with special reference to Morocco. Provo, Utah, USA, Brigham Young University.Google Scholar
Craig, P., Rogan, M. & Allan, J. (1996) Detection, screening and community epidemiology of taeniid cestode zoonosis: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Advances in Parasitology 38, 169–250.CrossRefGoogle ScholarPubMed
Dada, B., Adegboys, S. & Mohammed, N. (1981) Experience in northern Nigeria with counter current immunoelectrophoresis, double diffusion and indirect hemagglutination tests for diagnosis of hydatid cyst in camels. Journal of Helminthology 55, 197–202.CrossRefGoogle ScholarPubMed
Farag, H., Bout, D. & Capron, A. (1975) Specific immunodiagnosis of human hydatidosis by the enzyme-linked immunosorbent assay (ELISA). Biomedicine 23, 276–278.Google Scholar
Fuller, G. & Fuller, D. (1981) Hydatid disease in Ethiopia: clinical survey with some immunodiagnostic test results. American Journal of Tropical Medicine and Hygiene 30, 645–652.CrossRefGoogle ScholarPubMed
Gatti, A., Rosa Alvarez, A., Araya, D., Mancini, S., Herrero, E., Santillan, G. & Larrieu, E. (2007) Ovine echinococcosis: immunological diagnosis by enzyme immunoassay. Veterinary Parasitology 143, 112–121.CrossRefGoogle ScholarPubMed
Gaym, A., Abebe, D. & Degefe, D. (2002) Hydatid cyst an unusual cause of ovarian enlargement. Ethiopian Medical Journal 40, 283–291.Google ScholarPubMed
Getaw, A., Beyene, D., Ayana, D., Megersa, B. & Abunna, F. (2010) Hydatidosis: prevalence and its economic importance in ruminants slaughtered at Adama municipal abattoir, Central Oromia, Ethiopia. Acta Tropica 113, 221–225.CrossRefGoogle ScholarPubMed
Hamel, K. & Ris, D. (1982) The use of cathodic antigen in immunoelectrophoretic serodiagnosis of Echinococcus granulosus in sheep. Veterinary Immunology and Immunopathology 3, 419–425.CrossRefGoogle ScholarPubMed
Hira, P., Bahr, M., Shweiki, M. & Behbehani, K. (1990) An enzyme linked immunosorbent assay using an arc 5 antigen for the diagnosis of cystic hydatid disease. Annals of Tropical Medicine and Parasitology 84, 157–162.CrossRefGoogle ScholarPubMed
Iacona, A., Pini, C. & Vicari, G. (1980) Enzyme-linked immunosorbent assay (ELISA) in serodiagnosis of hydatid disease. American Journal of Tropical Medicine and Hygiene 29, 95–102.CrossRefGoogle ScholarPubMed
Ibrahem, M., Craig, S. & Mcvie, A. (1996) Echinococcus granulosus antigen B and seroreactivity in natural ovine hydatidosis. Research in Veterinary Science 61, 102–106.CrossRefGoogle ScholarPubMed
Ibrahem, M., Rafiei, A., Dar, F., Azwai, S., Carter, S. & Craig, P. (2002) Serodiagnosis of cystic echinococcosis in naturally infected camels. Parasitology 125, 245–251.CrossRefGoogle ScholarPubMed
Irabuena, O., Nieto, A., Ferreira, A., Battistoni, J. & Ferragut, G. (2000) Characterization and optimization of bovine Echinococcus granulosus cyst fluid to be used in immunodiagnosis of hydatid disease by ELISA. Revista do Instituto de Medicina Tropical de São Paulo 42, 255–262.CrossRefGoogle ScholarPubMed
Ito, A., Ma, L., Schantz, P., Gottstein, B., Liu, Y., Chai, J., Abdelhafez, S., Altintas, N., Joshi, D., Lightowlers, M. & Pawloski, Z. (1999) Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and Echinococcus multilocularis protoscolex (EM18). American Journal of Tropical Medicine and Hygiene 60, 188–192.CrossRefGoogle ScholarPubMed
Jenkins, D., Romig, T. & Thompson, R. (2005) Emergence/re-emergence of Echinococcus spp. a global update. International Journal for Parasitology 35, 1205–1219.CrossRefGoogle ScholarPubMed
Jobre, Y., Labag, F., Tirone, R., Abebe, G. & Dorchies, P. (1996) Hydatidosis in three selected regions in Ethiopia: an assessment trial on its prevalence, economic and public health importance. Revue de Médecine Vétérinaire 147, 797–804.Google Scholar
Kebede, N., Mekonnen, H., Wossene, A. & Tilahun, G. (2008) Hydatidosis of slaughtered cattle in Wolaita Sodo Abattoir, southern Ethiopia. Tropical Animal Health and Production 41, 629–633.CrossRefGoogle ScholarPubMed
Kebede, W., Hagos, A., Girma, Z. & Lobago, F. (2009) Echinococcosis/hydatidosis: its prevalence, economic and public health significance in Tigray region, North Ethiopia. Tropical Animal Health and Production 41, 865–871.CrossRefGoogle Scholar
Khan, M., Afzal, M. & Ali, S. (1990) Prevalence and serology of hydatidosis in large ruminants of Pakistan. Veterinary Parasitology 37, 163–168.CrossRefGoogle ScholarPubMed
Kittelberger, R., Reichel, M., Jenner, J., Heath, D., Lightowlers, M., Moro, P., Ibrahem, M., Craig, P. & O'Keefe, J. (2002) Evaluation of three enzyme-linked immunosorbent assays for the detection of serum antibodies in sheep infected with Echinococcus granulosus. Veterinary Parasitology 110, 57–76.CrossRefGoogle ScholarPubMed
Larrieu, E., Costa, M., Cantoni, G., Alvarez, R., Cavagion, L., Labanchi, J., Bigatti, R., Raya, D., Herrero, E., Mancini, S. & Cabrera, A. (2001) Ovine Echinococcus granulosus transmission dynamics in the province of Rio Negro, Argentina. Veterinary Parasitology 98, 263–272.CrossRefGoogle ScholarPubMed
Lightowlers, M. & Gottstein, B. (1995) Echinococcosis/hydatidosis: antigens, immunological and molecular diagnosis. pp. 355–410in Thompson, R. & Lymbery, A. (Eds) Echinococcus and hydatid disease. Wallingford, UK, CAB International.Google Scholar
Lightowlers, M., Rickard, M., Honey, R., Obendorf, D. & Mitchell, G. (1984) Serological diagnosis of Echinococcus granulosus infection in sheep using cyst fluid antigen processed by antibody affinity chromotograph. Australian Veterinary Journal 61, 101–108.CrossRefGoogle Scholar
Lowry, O., Rosebrough, N., Farr, A. & Randall, R. (1951) Protein measurement with the phenol reagent. The Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Margutti, P., Ortona, E., Vaccari, S., Barca, S., Rigano, R., Teggi, A., Muhschlegel, F., Frosch, M. & Siracusano, A. (1999) Cloning and expression of a cDNA encoding an elongation factor 1beta/delta protein from Echinococcus granulosus with immunogenic activity. Parasite Immunology 21, 485–492.CrossRefGoogle ScholarPubMed
McManus, D. & Smyth, J. (1986) Hydatid disease (hydatidosis): changing concepts in epidemiology and speciation. Parasitology Today 2, 163–168.CrossRefGoogle ScholarPubMed
Minas, M., Biluts, H., Bekele, A. & Alemie, M. (2007) Surgical management of 234 patients with hydatid disease: The Tikur Anbessa Hospital experience. Ethiopian Medical Journal 45, 257–265.Google ScholarPubMed
Moose, R. & Abdel-Hafez, S. (1994) Serodiagnosis and seroepidemiology of human unilocular hydatidosis in Jordan. Parasitology Research 80, 664–761.CrossRefGoogle Scholar
Moro, P., Verastagui, M., Gilman, R.H., Falcon, N., Bernal, T., Gavidia, C., Gonzalez, A., Malqui, V., Moro, M. & Dueger, E. (1997) Enzyme-linked immunoelectrotransfer blot assay for diagnosis of hydatidosis (Echinococcus granulosus) in sheep. The Veterinary Record 140, 605–606.CrossRefGoogle ScholarPubMed
Nasrieh, M. & Abdel-Hafez, S. (2004) Echinococcus granulosus in Jordan: assessment of various antigenic preparations for use in the serodiagnosis of surgically confirmed cases using enzyme immuno assays and the indirect haemagglutination test. Diagnostic Microbiology and Infectious Disease 48, 117–123.CrossRefGoogle ScholarPubMed
Njoroge, E., Mbithi, P., Gathumaa, M., Wachira, M., Gathura, B., Magamboc, K. & Zeyhle, E. (2002) A study of cystic echinococcosis in slaughter animals in three selected areas of northern Turkana. Kenya Veterinary Parasitology 104, 85–91.CrossRefGoogle ScholarPubMed
Poretti, D., Felleisen, E., Grimm, F., Pfister, M., Teuscher, F., Zuercher, C., Reichen, J. & Gottstein, B. (1999) Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathology. American Journal of Tropical Medicine and Hygiene 60, 193–198.CrossRefGoogle Scholar
Ragassa, F., Molla, A. & Bekele, J. (2010) Study on the prevalence of cystic hydatidosis and its economic significance in cattle slaughtered at Hawassa Municipal abattoir, Ethiopia. Tropical Animal Health and Production 42, 977–984.CrossRefGoogle Scholar
Sadjjadi, S., Abidi, H., Sarkari, B., Izadpanah, A. & Kazemian, S. (1986) Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiagnosis of human hydatidosis. International Journal of Zoonoses 13, 241–245.Google Scholar
Sbihi, Y., Rmigui, A., Maria, R., Orduna, A., Rodrigues-Torres, A. & Osuna, A. (2001) Comparative sensitivity of six serological tests and diagnostic value of ELISA using purified antigen in hydatidosis. Journal of Clinical Laboratory Analysis 15, 14–18.3.0.CO;2-7>CrossRefGoogle ScholarPubMed
Schantz, P., Chai, J., Craig, P., Eckert, J., Jenkins, D., MacPherson, C. & Thakur, A. (1995) Epidemiology and control of hydatid disease. pp. 233–331in Thompson, R. & Lymbery, A. (Eds) Echinococcus and hydatid disease. Wallingford, UK, CAB International.Google Scholar
Soulsby, E. (1982) Helminth, arthropod and protozoa of domestic animals. 7th edn.Philadelphia, USA, Lea & Fabiger.Google Scholar
Voller, A., Bidwell, E. & Bartlett, A. (1976) Enzyme immunoassay in diagnostic medicine. Bulletin of World Health Organization 53, 55–63.Google ScholarPubMed
Yong, W., Health, D. & Knapen, F. (1984) Comparison of cestode antigens in an enzyme-linked immunosorbent assay for the diagnosis of Echinococcus granulosus, Taenia hydatigena and T. ovis infection in sheep. Research in Veterinary Science 36, 24–31.CrossRefGoogle Scholar