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Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): stability and reaction conditions for prospective field tests

Published online by Cambridge University Press:  05 June 2009

Mohamed A. Idris  and
Andreas Ruppel
Mohamed A. Idris
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
Andreas Ruppel*
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
*
**Correspondence: Dr A. Ruppel, Institut für Tropenhygiene, Im Neuenheimer Feld 324, D-6900 Heidelberg, F.R.G.
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Abstract

The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm31/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

Keywords

diagnostic proteinsstabilityreaction conditionsSchistosoma haematobiumSh31/32Schistosoma mansoniSm31/32
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 2 , June 1991 , pp. 89 - 94
Copyright
Copyright © Cambridge University Press 1991

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