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A note on the estimation of D(–) lactic acid using lactic dehydrogenase

Published online by Cambridge University Press:  01 June 2009

Ellen I. Garvie
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading

Extract

Van den Hamer & Elias (1958) described a method of estimating D(–) lactic acid using lactic dehydrogenase and a Warburg technique. The enzyme was obtained as an acetone preparation of Escherichia coli grown on a medium containing lactate as energy source.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1966

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References

REFERENCES

Barker, J. B. & Summerson, W. H. (1941). J. biol. Chem. 138, 535.CrossRefGoogle Scholar
Gercken, G. (1960). Hoppe-Seyler's Z. physiol. Chem. 320, 180.Google Scholar
Haugaard, N. (1959). Biochim. biophys. Acta, 31, 66.CrossRefGoogle Scholar
Kline, E. S. & Mahler, H. R. (1965). Ann. N.Y. Acad. Sci. 119, (3), 905.CrossRefGoogle Scholar
Van den Hamer, C. J. A. & Elias, R. W. (1958). Biochim. biophys. Acta, 29, 556.Google Scholar