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Metabolism of phenylalanine and biosynthesis of styrene in Penicillium camemberti

Published online by Cambridge University Press:  12 February 2007

Yves Pagot ,
Jean-Marc Belin ,
Florence Husson  and
Henry-Eric Spinnler
Yves Pagot
Affiliation:
UMR de Microbiologie et Génétique Moléculaire, Institut National Agronomique Paris-Grignon, UMR INRA 537, URA CNRS 1925, F-78850 Thiverval-Grignon France
Jean-Marc Belin
Affiliation:
Laboratoire de Microbioologie, UMR INRA 1283 Ecole Nationale Supérieure de Biologie Appliquée à la. Nutrition et à l'Alimentation, 1, Esplanade Erasme, F-21000 Dijon, France
Florence Husson
Affiliation:
Laboratoire de Microbioologie, UMR INRA 1283 Ecole Nationale Supérieure de Biologie Appliquée à la. Nutrition et à l'Alimentation, 1, Esplanade Erasme, F-21000 Dijon, France
Henry-Eric Spinnler
Affiliation:
UMR de Génie et Microbiologie des Procédés et Alimentaires, Institut National Agronomique Paris-Grignon, UMR INRA GMPA, F-78850 Thiverval-Grignon, France
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Abstract

The occurrence of styrene in food may be an important aroma defect (celluloid odour), even at very low concentrations (Miltz et al. 1980) causing consumer rejection and is therefore a problem for the food industry. We examined the biosynthetic pathway leading to styrene formation by Penicillium camemberti using labelled compounds. As styrene is strongly hydrophobic and volatile, we first had to develop a continuous extraction process. Using resins XAD2 it was reasonable to suspect phenylalanine (Phe) as the precursor. The addition of Phe marked with 13C on the ring provokes the accumulation of labelled styrene. The enzyme activities involved were also tentatively measured. Styrene appears to be synthesized from phenylalanine by phenylalanine ammonia-lyase activity followed by a decarboxylation catalysed by a cinnamic acid decarboxylase.

Keywords

Styrene productionPhenylalanine catabolismPenicillium camemberti
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 2 , May 2007 , pp. 180 - 185
Copyright
Proprietors of Journal of Dairy Research 2007

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