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Measurements in vivo of the rate of production of protozoa in the rumen

Published online by Cambridge University Press:  01 June 2009

U. B. Singh ,
A. Varma ,
D. N. Verma  and
S. K. Ranjhan
U. B. Singh
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India
A. Varma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India
D. N. Verma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India
S. K. Ranjhan
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India
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Summary

The rates of production of protozoa in the rumen of buffalo calves have been estimated using a single-injection isotope dilution technique. The calves were fed at 2 levels of crude protein, namely, 13% lower and 19% higher than that recommended by the National Research Council. The animals were given their rations at 2-h intervals for 3 weeks. Thereafter 14C-labelled rumen protozoa were injected into the rumen of each calf in a single injection. Samples of the rumen liquor were drawn at various time-intervals for 10 h and were analysed for the concentration of protozoa and radioactivity. The decline in the specific radioactivity of protozoa cells in the rumen as a function of time was used for calculating half life (t½). A mathematical equation was used to calculate the rate of production of protozoa. The average t½ was 1077 min for both groups and the rates of production of protozoa were 73·9 and 92·1mg/min in groups fed on low and high planes of crude protein respectively, showing that the production of protozoa is significantly (P < 0·01) higher in animals consuming a ration high in crude protein.

Type
Research Article
Information
Journal of Dairy Research , Volume 41 , Issue 3 , October 1974 , pp. 299 - 303
Copyright
Copyright © Proprietors of Journal of Dairy Research 1974

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References

REFERENCES

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