Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-26T04:47:09.541Z Has data issue: false hasContentIssue false
  • English
  • Français

Early post parturient changes in milk acute phase proteins

Published online by Cambridge University Press:  07 September 2016

Funmilola C Thomas ,
Mary Waterston ,
Peter Hastie ,
Hayley Haining  and
P David Eckersall
Funmilola C Thomas
Affiliation:
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Nigeria
Mary Waterston
Affiliation:
Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Glasgow, UK
Peter Hastie
Affiliation:
School of Veterinary Medicine, University of Glasgow, Glasgow, UK
Hayley Haining
Affiliation:
School of Veterinary Medicine, University of Glasgow, Glasgow, UK
P David Eckersall*
Affiliation:
Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow, UK
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The periparturient period is one of the most critical periods in the productive life of a dairy cow, and is the period when dairy cows are most susceptible to developing new intramammary infections (IMI) leading to mastitis. Acute phase proteins (APP) such as haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) have been detected in milk during mastitis but their presence in colostrum and milk in the immediate postpartum period has had limited investigation. The hypothesis was tested that APP are a constituent of colostrum and milk during this period. Enzyme linked immunosorbent assays (ELISAs) were used to determine each APP's concentration in colostrum and milk collected daily from the first to tenth day following calving in 22 Holstein-Friesian dairy cows. Haptoglobin was assessed in individual quarters and composite milk samples while M-SAA3 and CRP concentration were determined in composite milk samples. Change in Hp in relation to the high abundance proteins during the transition from colostrum to milk were evaluated by 1 and 2 dimension electrophoresis and western blot. In 80% of the cows all APPs were detected in colostrum on the first day following parturition at moderately high levels but gradually decreased to minimal values in the milk by the 6th day after calving. The remaining cows (20%) showed different patterns in the daily milk APP concentrations and when an elevated level is detected could reflect the presence of IMI. Demonstration that APP are present in colostrum and milk following parturition but fall to low levels within 4 days means that elevated APP after this time could be biomarkers of post parturient mastitis allowing early intervention to reduce disease on dairy farms.

Keywords

Post-calvinghaptoglobinmammary associated serum amyloid A3C-reactive proteindairy cowmastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 352 - 359
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alsemgeest, SPM, Jonker, FH, Taverne, MAM, Kalsbeek, HC, Wensing, T & Gruys, E 1995 Serum Amyloid-A (Saa) and Haptoglobin (Hp) Plasma-concentrations in Newborn Calves. Theriogenology 43 381–387 Google Scholar
Barkema, HW 1999 Quarter-milk somatic cell count at calving and at the first six milkings after calving. Preventive Veterinary Medicine 38 1–9 Google Scholar
Braceland, M, Bickerdike, R, Tinsley, J, Cockerill, D, Mcloughlin, MF, Graham, DA, Burchmore, RJ, Weir, W, Wallace, C & Eckersall, PD 2013 The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3). Journal of Proteomics 94 423–436 CrossRefGoogle ScholarPubMed
Braceland, M, McLoughlin, MF, Tinsley, J, Wallace, C, Cockerill, D, McLaughlin, M & Eckersall, PD 2014 Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L. Journal of Fish Diseases 38 821–831 Google Scholar
Edwards, PJB & Jameson, GB 2014 Structure and stability of whey proteins. In Milk Proteins From Expression to Food, 2nd edition, pp. 201–243 (Eds Singh, H, Boland, M & Thompson, A). Amsterdam: Academic Press Google Scholar
Ceciliani, F, Pocacqua, V, Provasi, E, Comunian, C, Bertolini, A, Bronzo, V, Moroni, P & Sartorelli, P 2005 Identification of the bovine alpha 1-acid glycoprotein in colostrum and milk. Veterinary Research 36 735–746 CrossRefGoogle ScholarPubMed
Dingwell, RT, Leslie, KE, Schukken, YH, Sargeant, JM & Timms, LL 2003 Evaluation of the California mastitis test to detect an intramammary infection with a major pathogen in early lactation dairy cows. Canadian Veterinary Journal-Revue Veterinaire Canadienne 44 413–415 Google Scholar
Eckersall, PD, Young, FJ, Nolan, AM, Knight, CH, McComb, C, Waterston, MM, Hogarth, CJ, Scott, EM & Fitzpatrick, JL 2006 Acute phase proteins in bovine milk in an experimental model of Staphylococcus aureus subclinical mastitis. Journal of Dairy Science 89 1488–1501 Google Scholar
Esposito, G, Irons, PC, Webb, EC & Chapwanya, A 2014 Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows. Animal Reproduction Science 144 60–97 Google Scholar
Fetherston, CM, Wells, JI & Hartmann, PE 2006 Severity of mastitis symptoms as a predictor of C-reactive protein in milk and blood during lactation. Breastfeeding Medicine 1 127–135 Google Scholar
Gronlund, U, Hallen Sandgren, C & Persson Waller, K 2005 Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis. Veterinary Research 36 191–198 Google Scholar
Hernandez-Castellano, LE, Almeida, AM, Castro, N & Arguello, A 2014 The colostrum proteome, ruminant nutrition and immunity: a review. Current Protein & Peptide Science 15 64–74 Google Scholar
Hiss, S, Mielenz, M, Bruckmaier, RM & Sauerwein, H 2004 Haptoglobin concentrations in blood and milk after endotoxin challenge and quantification of mammary Hp mRNA expression. Journal of Dairy Science 87 3778–3784 Google Scholar
Hiss, S, Weinkauf, C, Hachenberg, S & Sauerwein, H 2009 Relationship between metabolic status and the milk concentrations of haptoglobin and lactoferrin in dairy cows during early lactation. Journal of Dairy Science 92 4439–4443 Google Scholar
Hogarth, CJ, Fitzpatrick, JL, Nolan, AM, Young, FJ, Pitt, A & Eckersall, PD 2004 Differential protein composition of bovine whey: a comparison of whey from healthy animals and from those with clinical mastitis. Proteomics 4 2094–2100 CrossRefGoogle ScholarPubMed
Humblet, MF, Guyot, H, Boudry, B, Mbayahi, F, Hanzen, C, Rollin, F & Godeau, JM 2006 Relationship between haptoglobin, serum amyloid A, and clinical status in a survey of dairy herds during a 6-month period. Veterinary Clinical Pathology 35 188–193 CrossRefGoogle Scholar
Jovanovic, S, Barac, M, Macej, O, Vucic, T & Lacnjevac, C 2007 SDS-PAGE analysis of soluble proteins in reconstituted milk exposed to different heat treatments. Sensors 7 371–383 CrossRefGoogle Scholar
Lai, IH, Tsao, JH, Lu, YP, Lee, JW, Zhao, X, Chien, FL & Mao, SJT 2009 Neutrophils as one of the major haptoglobin sources in mastitis affected milk. Veterinary Research 40 17 Google Scholar
Lee, WC, Hsiao, HC, Wu, YL, Lin, JH, Lee, YP, Fung, HP, Chen, HH, Chen, YH & Chu, RM 2003 Serum C-reactive protein in dairy herds. Canadian Journal of Veterinary Research-Revue Canadienne de Recherche Veterinaire 67 102–107 Google Scholar
McDonald, TL, Larson, MA, Mack, DR & Weber, A 2001 Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum. Veterinary Immunology and Immunopathology 83 203–211 Google Scholar
Morimatsu, M, Watanabe, A, Yoshimatsu, K, Fujinaga, T, Okubo, M & Naiki, M 1991 Elevation of bovine serum C-reactive protein and serum amyloid-P component levels by lactation. Journal of Dairy Research 58 257–261 Google Scholar
O'Mahony, JA 2014 Milk: an overview. In Milk Proteins from Expression to Food, 2nd edition, pp. 20–75 (Eds Singh, H, Boland, M & Thompson, A). Amsterdam: Academic Press Google Scholar
Pyorala, S, Hovinen, M, Simojoki, H, Fitzpatrick, J, Eckersall, PD & Orro, T 2011 Acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens. Veterinary Record 168 535 Google Scholar
Sanchez, L, Aranda, P, Perez, MD & Calvo, M 1988 Concentration of Lactoferrin and transferrin throughout lactation in cows colostrum and milk. Biological Chemistry Hoppe-Seyler 369 1005–1008 Google Scholar
Sargeant, JM, Shirley, JE, Pulkrabek, BJ, Lim, GH & Leslie, KE 2001 Sensitivity and specificity of somatic cell count and California Mastitis Test for identifying intramammary infection in early lactation. Journal of Dairy Science 84 2018–2024 Google Scholar
Schrödl, W, Krüger, M, Hien, TT, Füldner, M & Kunze, R 1995 C-reactive protein as a new parameter of mastitis. Tierärztliche Prax 23 337–341 Google Scholar
Schroedl, W, Jaekel, L & Krueger, M 2003 C-reactive protein and antibacterial activity in blood plasma of colostrum-fed calves and the effect of lactulose. Journal of Dairy Science 86 3313–3320 Google Scholar
Thomas, FC, Waterston, M, Hastie, P, Parkin, T, Haining, H & Eckersall, PD 2015 The major acute phase proteins of bovine milk in a commercial dairy herd. BMC Veterinary Research 11 207 Google Scholar
Trevisi, E, Zecconi, A, Bertoni, G & Piccinini, R 2010 Blood and milk immune and inflammatory profiles in periparturient dairy cows showing a different liver activity index. Journal of Dairy Research 77 310–317 Google Scholar
Trevisi, E, Amadori, M, Cogrossi, S, Razzuoli, E & Bertoni, G 2012 Metabolic stress and inflammatory response in high-yielding, periparturient dairy cows. Research in Veterinary Science 93 695–704 Google Scholar
Tothova, C, Nagy, O, Seidel, H & Kovac, G 2012 The effect of storage temperature and time on the concentration of bovine serum amyloid A and its mammary isoform. Veterinary Medicine International Article ID 861458, 1–6 Google Scholar
Uchida, E, Katoh, N & Takahashi, K 1993 Appearance of haptoglobin in serum from cows at parturition. Journal of Veterinary Medical Science 55 893–894 Google Scholar
Waldron, MR & Revelo, XS 2008 Causes and effects immunosuppression of periparturient aspects of periparturient metabolism immunosuppression. WCDS Advances in Dairy Technology 20 97–109 Google Scholar
Yuan, K, Farney, JK, Mamedova, LK, Sordillo, LM & Bradford, BJ 2013 TNF altered inflammatory responses, impaired health and productivity, but did not affect glucose or lipid metabolism in early-lactation dairy cows. PLoS One 8 33–37 Google Scholar
Zimmermann, S, Neumann, A, Kruger, M, Furll, M & Elze, K 1998 Clinical course of puerperium and metabolic parameters with special reference to c-reactive protein as criterions to the events of conception in the new reproductive period in dairy cows. Zuchtungskunde 70 261–281 Google Scholar
Zhang, L, Boeren, S, Hageman, JA, van Hooijdonk, T, Vervoort, J & Hettinga, K 2015 Bovine milk proteome in the first 9 days: protein interactions in maturation of the immune and digestive system of the newborn. PLOS One e0116710 1–19 Google Scholar