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Determination of leucocyte concentrations in cow's milk with a Coulter counter

Published online by Cambridge University Press:  01 June 2009

L. W. Phipps  and
F. H. S. Newbould
L. W. Phipps
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
F. H. S. Newbould
Affiliation:
Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
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Summary

A technique for determining the concentration of leucocytes in cow's milk is described which is more rapid and accurate as well as much less tedious than conventional microscope methods. The leucocytes are isolated from the fat globules of comparable size by a novel centrifuging procedure. Sizing of the leucocytes with a Coulter electronic counter shows that their volumes range from about 45 to 1770 µm3 but a partially overlapping distribution of other particulate matter prevents the accurate determination of the total number of cells present. The numbers counted in truncated leucocyte distributions, however, correlate linearly with total cell counts determined with an improved microscope method up to 6 × 106 cells/ml of milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 33 , Issue 1 , February 1966 , pp. 51 - 64
Copyright
Copyright © Proprietors of Journal of Dairy Research 1966

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References

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