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Density-gradient electrophoresis of native and of rennet-treated casein micelles

Published online by Cambridge University Press:  01 June 2009

Margaret L. Green  and
G. Crutchfield
Margaret L. Green
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
G. Crutchfield
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
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Summary

Analytical electrophoresis of native and of rennet-treated casein micelles was carried out at 26 and at 5°C in a sucrose density gradient in a medium of the same ionic composition as milk. Under normal circumstances, the micelles were negatively charged and showed little heterogeneity in the electric field, the fastestmoving ones having at 26°C a mobility not more than 30% greater than that of the slowest. Native micelles were more highly charged at 5 than at 26°C; at each temperature, the mobility was approximately halved by rennet treatment. The results suggest that the effects of rennet treatment and of alterations in temperature on the tendency of the micelles to clot can be entirely explained as being caused by changes in charge.

Type
Original Articles
Information
Journal of Dairy Research , Volume 38 , Issue 2 , June 1971 , pp. 151 - 164
Copyright
Copyright © Proprietors of Journal of Dairy Research 1971

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