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A coagulase-negative variant of Staphylococcus aureus from bovine mastitis milk

Published online by Cambridge University Press:  01 December 2010

Ömer Akineden ,
Abdulwahed Ahmed Hassan ,
Elisabeth Schneider  and
Ewald Usleber
Ömer Akineden*
Affiliation:
Food Science, Institute of Veterinary Food Science, Justus-Liebig-University Giessen, Frankfurter Strasse 92, D-35392 Giessen, Germany
Abdulwahed Ahmed Hassan
Affiliation:
GD Animal Health Service, Department of Bacteriology and Parasitology, Postbus 9, 7400 AA Deventer, The Netherlands
Elisabeth Schneider
Affiliation:
Dairy Sciences, Institute of Veterinary Food Science, Justus-Liebig-University Giessen, Ludwigstrasse 21, D-35390 Giessen, Germany
Ewald Usleber
Affiliation:
Dairy Sciences, Institute of Veterinary Food Science, Justus-Liebig-University Giessen, Ludwigstrasse 21, D-35390 Giessen, Germany
*
*For correspondence: [email protected]
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Abstract

Bacteriological analysis of milk samples from quarters of a dairy cow suffering from subclinical mastitis yielded two isolates of Staphylococcus aureus which gave a negative reaction in the standard coagulase test. Both isolates were also clumping factor and thermonuclease negative, and gave a negative reaction in the Staphaurex® test. The isolates were identified by using commercial biochemical systems, and by PCR analysis of different staphylococcal cell surface protein and exoprotein genes. Further molecular identification of the isolates, which included sequencing of the 16S rRNA gene and RT-PCR of coagulase (coa), clumping-factor (clfA) and thermonuclease (nuc) genes, was consistent with the diagnosis phenotypically ‘coagulase-negative variant of Staph. aureus’. The fact that coagulase-negative Staph. aureus variants can occur in the context of intramammary infections in cattle may result in the incorrect diagnosis ‘coagulase-negative staphylococci (CNS)’ in routine mastitis diagnostic, at least in rare cases. To fully ensure correct species diagnosis, sequencing of the 16S rRNA gene and amplification of specific genes such as coa is necessary in these cases.

Keywords

Staphylococcus aureuscoagulase negativebovine mastitisbeta-haemolysin
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 1 , February 2011 , pp. 38 - 42
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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