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Antimicrobial (ESBL) resistance genes in faecal E. coli of calves fed waste milk with antimicrobial residues

Published online by Cambridge University Press:  30 August 2022

Manuel Cardoso ,
Inês Prata ,
Inês Rebelo ,
Telmo Nunes ,
Ana Pires ,
Carla Carneiro  and
Ricardo Bexiga
Manuel Cardoso
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
Inês Prata
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal HVME – Hospital Veterinário Muralha de Évora, Évora, Portugal
Inês Rebelo
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
Telmo Nunes
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
Ana Pires
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
Carla Carneiro
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
Ricardo Bexiga*
Affiliation:
Faculty of Veterinary Medicine, CIISA – Centre for Interdisciplinary Research in Animal Health, University of Lisbon, Lisbon, Portugal Faculty of Veterinary Medicine, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Lisbon, Lisbon, Portugal
*
Author for correspondence: Ricardo Bexiga, Email: [email protected]
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Abstract

This research paper aimed to evaluate the association between feeding waste milk to calves and the occurrence of antimicrobial multi-resistance by extended spectrum β-lactamase (ESBL) enzymes through determining their production by E. coli isolates from 32 dairy farms. Among β-lactamase enzymes, ESBL provide resistance to a wide variety of β-lactam antimicrobials including penicillin and 2nd, 3rd and 4th generation cephalosporins. Feeding waste milk to calves has been observed to lead to increased antimicrobial resistance in faecal isolates of calves. In each farm included in this study, faecal samples were collected from the rectum of five healthy calves in the first month of life and pooled into a single container. Five isolates from each pool were selected and confirmed to be E. coli by amplification of the 16S rRNA gene. ESBL production was confirmed phenotypically on 148 isolates from 31 farms by use of the double-disk synergy test. Genotypic confirmation of ESBL production was performed by PCR for the genes blaCTX-M-1, −2, −8, −9 and blaCMY-2. A questionnaire was also performed and a mixed logistic regression model was used to identify risk factors for the occurrence of antimicrobial resistance. A negative binomial regression model was also used, in order to assess whether there was any association between certain farm management practices and the number of ESBL-producing E. coli isolates from each farm. Phenotypic confirmation of ESBL production was obtained on 40 E. coli isolates from 15 farms (48.4%), whereas genotypic confirmation was obtained on 55 isolates from 20 farms (64.5%). The use of three or more different intramammary antimicrobials to treat mastitis within the previous year significantly impacted the number of ESBL-producing E. coli isolates; on farms that did so, there were more isolates in which ESBL-producing E. coli was present, when compared to farms that had used less formulations within the same time span.

Keywords

AntimicrobialcalvesE. coliESBLwaste milk
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 3 , August 2022 , pp. 259 - 264
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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