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Administration of a live culture of Lactococcus lactis DPC 3147 into the bovine mammary gland stimulates the local host immune response, particularly IL-1β and IL-8 gene expression

Published online by Cambridge University Press:  18 May 2009

Christine Beecher ,
Mairéad Daly ,
Donagh P Berry ,
Katja Klostermann ,
James Flynn ,
William Meaney ,
Colin Hill ,
Tommie V McCarthy ,
R Paul Ross  and
Linda Giblin
Christine Beecher
Affiliation:
Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland Biochemistry Department, University College Cork, Cork, Ireland
Mairéad Daly
Affiliation:
Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
Donagh P Berry
Affiliation:
Moorepark Production Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
Katja Klostermann
Affiliation:
Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland Microbiology Department, University College Cork, Cork, Ireland
James Flynn
Affiliation:
Moorepark Production Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
William Meaney
Affiliation:
Moorepark Production Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
Colin Hill
Affiliation:
Microbiology Department, University College Cork, Cork, Ireland
Tommie V McCarthy
Affiliation:
Biochemistry Department, University College Cork, Cork, Ireland
R Paul Ross
Affiliation:
Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
Linda Giblin*
Affiliation:
Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland
*
*For correspondence; e-mail: [email protected]
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Abstract

Mastitis is one of the most costly diseases to the dairy farming industry. Conventional antibiotic therapy is often unsatisfactory for successful treatment of mastitis and alternative treatments are continually under investigation. We have previously demonstrated, in two separate field trials, that a probiotic culture, Lactococcus lactis DPC 3147, was comparable to antibiotic therapy to treat bovine mastitis. To understand the mode of action of this therapeutic, we looked at the detailed immune response of the host to delivery of this live strain directly into the mammary gland of six healthy dairy cows. All animals elicited signs of udder inflammation 7 h post infusion. At this time, clots were visible in the milk of all animals in the investigation. The most pronounced increase in immune gene expression was observed in Interleukin (IL)-1β and IL-8, with highest expression corresponding to peaks in somatic cell count. Infusion with a live culture of a Lc. lactis leads to a rapid and considerable innate immune response.

Keywords

MastitisLactococcus lactisprobiotic treatment
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 3 , August 2009 , pp. 340 - 348
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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