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Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers

Published online by Cambridge University Press:  01 August 2012

Joren Verbeke ,
Sofie Piepers ,
Luc Peelman ,
Mario Van Poucke  and
Sarne De Vliegher
Joren Verbeke*
Affiliation:
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
Sofie Piepers
Affiliation:
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
Luc Peelman
Affiliation:
Laboratory for Animal Genetics, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
Mario Van Poucke
Affiliation:
Laboratory for Animal Genetics, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
Sarne De Vliegher
Affiliation:
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
*
*For correspondence; email: [email protected]
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Abstract

The chemokine (C-X-C motif) receptor 1 (CXCR1) gene encodes the homonymous receptor for interleukin 8 (IL8) on polymorphonuclear neutrophilic leucocytes (PMNL). Binding causes migration from blood to milk, activation and prolonged survival of PMNL, a crucial process in the innate immune defence of the bovine mammary gland against invading mastitis-causing pathogens. The main objective of this study was to screen the entire coding region of the CXCR1 gene for polymorphisms and to analyse their association with udder health of dairy heifers. One-hundred-and-forty Belgian Holstein heifers originating from 20 commercial dairy farms were genotyped by DNA sequencing. Detailed phenotypic data on udder health was available including quarter bacteriological culture results and somatic cell count (SCC) in early lactation and composite milk SCC during first lactation. In total, 16 polymorphisms (including 8 missense mutations) were detected. Polymorphism c.980A>G was associated with pathogen-group specific IMI: heifers with genotype AG were less likely to have an IMI due to major mastitis pathogens compared with heifers with genotype GG but did not have less IMI by coagulase-negative staphylococci, so-called minor pathogens. CXCR1 genotype was neither associated with quarter SCC in early lactation nor with composite SCC during lactation. Although mastitis susceptibility is influenced by many factors, some genetic polymorphisms potentially have major effects on udder health of heifers, as was shown here. These results trigger us to further study the relationship between CXCR1 polymorphisms and mastitis susceptibility in both observational and experimental trials.

Keywords

CXCR1 polymorphismsheifer mastitispolymorphonuclear neutrophilic leucocytes
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 3 , August 2012 , pp. 341 - 351
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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References

Altschul, SF, Gish, W, Miller, W, Myers, EW & Lipman, DJ 1990 Basic local alignment search tool. Journal of Molecular Biology 215 403–410 CrossRefGoogle ScholarPubMed
Barber, MR & Yang, TJ 1998 Chemotactic activities in nonmastitic and mastitic mammary secretions: Presence of interleukin-8 in mastitic but not nonmastitic secretions. Clinical and Diagnostic Laboratory Immunology 5 82–86 CrossRefGoogle Scholar
Beecher, C, Daly, M, Childs, S, Berry, DP, Magee, DA, McCarthy, TV & Giblin, L 2010 Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genetics 11 99 CrossRefGoogle ScholarPubMed
Birnboim, HC & Doly, J 1979 A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7 1513–1523 CrossRefGoogle ScholarPubMed
Caswell, JL, Middleton, DM & Gordon, JR 1999 Production and functional characterization of recombinant bovine interleukin-8 as a specific neutrophil activator and chemoattractant. Veterinary Immunology and Immunopathology 67 327–340 CrossRefGoogle ScholarPubMed
De Vliegher, S, Laevens, H, Barkema, HW, Dohoo, IR, Stryhn, H, Opsomer, G & de Kruif, A 2004 Management practices and heifer characteristics associated with early lactation somatic cell count of Belgian dairy heifers. Journal of Dairy Science 87 937–947 CrossRefGoogle ScholarPubMed
De Vliegher, S, Fox, LK, Piepers, S, McDougall, S & Barkema, HW 2012 Invited review: mastitis in dairy heifers: nature of the disease, potential impact, prevention, and control. Journal of Dairy Science 95 1025–1040.CrossRefGoogle ScholarPubMed
Detilleux, JC 2009 Genetic factors affecting susceptibility to udder pathogens. Veterinary Microbiology 134 157–164 CrossRefGoogle ScholarPubMed
Fox, LK 2009 Prevalence, incidence and risk factors of heifer mastitis. Veterinary Microbiology 134 82–88 CrossRefGoogle ScholarPubMed
Galvao, KN, Pighetti, GM, Cheong, SH, Nydam, DV & Gilbert, RO 2011 Association between interleukin-8 receptor-alpha (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. Journal of Dairy Science 94 2083–2091 CrossRefGoogle ScholarPubMed
Goertz, I, Baes, C, Weimann, C, Reinsch, N & Erhardt, G 2009 Association between single nucleotide polymorphisms in the CXCR1 gene and somatic cell score in Holstein dairy cattle. Journal of Dairy Science 92 4018–4022 CrossRefGoogle ScholarPubMed
Grosse, WM, Kappes, SM, Laegreid, WW, Keele, JW, Chitko-McKown, CG & Heaton, MP 1999 Single nucleotide polymorphism (SNP) discovery and linkage mapping of bovine cytokine genes. Mammalian Genome 10 1062–1069 CrossRefGoogle ScholarPubMed
Huijps, K, De Vliegher, S, Lam, T & Hogeveen, H 2009 Cost estimation of heifer mastitis in early lactation by stochastic modelling. Veterinary Microbiology 134 121–127 CrossRefGoogle ScholarPubMed
Kettritz, R, Gaido, ML, Haller, H, Luft, FC, Jennette, CJ & Falk, RJ 1998 Interleukin-8 delays spontaneous and tumor necrosis factor-alpha-mediated apoptosis of human neutrophils. Kidney International 53 84–91 CrossRefGoogle ScholarPubMed
Kromker, V & Friedrich, J 2009 Teat canal closure in non-lactating heifers and its association with udder health in the consecutive lactation. Veterinary Microbiology 134 100–105 CrossRefGoogle ScholarPubMed
Leyva-Baca, I, Schenkel, F, Martin, J & Karrow, NA 2008 Polymorphisms in the 5′ upstream region of the CXCR1 chemokine receptor gene, and their association with somatic cell score in Holstein cattle in Canada. Journal of Dairy Science 91 407–417 CrossRefGoogle ScholarPubMed
Mitchell, GB, Albright, BN & Caswell, JL 2003 Effect of interleukin-8 and granulocyte colony-stimulating factor on priming and activation of bovine neutrophils. Infection and Immunity 71 1643–1649 CrossRefGoogle ScholarPubMed
Myllys, V & Rautala, H 1995 Characterization of clinical mastitis in primiparous heifers. Journal of Dairy Science 78 538–545 CrossRefGoogle ScholarPubMed
Nash, DL, Rogers, GW, Cooper, JB, Hargrove, GL & Keown, JF 2003 Heritability of intramammary infections at first parturition and relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. Journal of Dairy Science 86 2684–2695 CrossRefGoogle ScholarPubMed
Ogorevc, J, Kunej, T, Razpet, A & Dovc, P 2009 Database of cattle candidate genes and genetic markers for milk production and mastitis. Animal Genetics 40 832–851 CrossRefGoogle ScholarPubMed
Paape, MJ, Shafer-Weaver, K, Capuco, AV, Van Oostveldt, K & Burvenich, C 2000 Immune surveillance of mammary tissue by phagocytic cells. Advances in Experimental Medicine and Biology 480 259–277 CrossRefGoogle ScholarPubMed
Piepers, S, De Meulemeester, L, de Kruif, A, Opsomer, G, Barkema, HW & De Vliegher, S 2007 Prevalence and distribution of mastitis pathogens in subclinically infected dairy cows in Flanders, Belgium. Journal of Dairy Research 74 478–483 CrossRefGoogle ScholarPubMed
Piepers, S, Opsomer, G, Barkema, HW, de Kruif, A & De Vliegher, S 2010 Heifers infected with coagulase-negative staphylococci in early lactation have fewer cases of clinical mastitis and higher milk production in their first lactation than noninfected heifers. Journal of Dairy Science 93 2014–2024 CrossRefGoogle ScholarPubMed
Piepers, S, Peeters, K, Opsomer, G, Barkema, HW, Frankena, K & De Vliegher, S 2011 Pathogen group specific risk factors at herd, heifer and quarter levels for intramammary infections in early lactating dairy heifers. Preventive Veterinary Medicine 99 91–101 CrossRefGoogle ScholarPubMed
Pighetti, GM, Kojima, CJ, Wojakiewicz, L & Rambeaud, M 2012 The bovine CXCR1 gene is highly polymorphic. Veterinary Immunology and Immunopathology 145 464–470 CrossRefGoogle ScholarPubMed
Pighetti, GM & Rambeaud, M 2006 Genome conservation between the bovine and human interleukin-8 receptor complex: Improper annotation of bovine interleukin-8 receptor b identified. Veterinary Immunology and Immunopathology 114 335–340 CrossRefGoogle ScholarPubMed
Sodeland, M, Kent, MP, Olsen, HG, Opsal, MA, Svendsen, M, Sehested, E, Hayes, BJ & Lien, S 2011 Quantitative trait loci for clinical mastitis on chromosomes 2, 6, 14 and 20 in Norwegian Red cattle. Animal Genetics 42 457–465 CrossRefGoogle Scholar
Svensson, C, Nyman, AK, Persson, WK & Emanuelson, U 2006 Effects of housing, management, and health of dairy heifers on first-lactation udder health in southwest Sweden. Journal of Dairy Science 89 1990–1999 CrossRefGoogle ScholarPubMed
Untergasser, A, Nijveen, H, Rao, X, Bisseling, T, Geurts, R & Leunissen, JAM 2007 Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Research 35 W71–W74 CrossRefGoogle ScholarPubMed
Van Poucke, M, Vandesompele, J, Mattheeuws, M, Van Zeveren, A & Peelman, LJ 2005 A dual fluorescent multiprobe assay for prion protein genotyping in sheep. BMC Infectious Diseases 5 13 CrossRefGoogle ScholarPubMed
Waage, S, Odegaard, SA, Lund, A, Brattgjerd, S & Rothe, T 2001 Case-control study of risk factors for clinical mastitis in postpartum dairy heifers. Journal of Dairy Science 84 392–399 CrossRefGoogle ScholarPubMed
Wanner, JM, Rogers, GW, Kehrli, ME & Cooper, JB 1998 Intramammary infections in primiparous Holsteins: heritabilities and comparisons of bovine leukocyte adhesion deficiency carriers and noncarriers. Journal of Dairy Science 81 3293–3299 CrossRefGoogle ScholarPubMed
Youngerman, SM, Saxton, AM, Oliver, SP & Pighetti, GM 2004a Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. Journal of Dairy Science 87 2442–2448 CrossRefGoogle ScholarPubMed
Youngerman, SM, Saxton, AM & Pighetti, GM 2004b Novel single nucleotide polymorphisms and haplotypes within the bovine CXCR2 gene. Immunogenetics 56 355–359 CrossRefGoogle ScholarPubMed
Zuker, M 2003 Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research 31 3406–3415 CrossRefGoogle ScholarPubMed