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A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gas-liquid chromatography

Published online by Cambridge University Press:  01 June 2009

Eric C. Needs ,
Graeme D. Ford ,
A. Jane Owen ,
Brian Tuckley  and
Malcolm Anderson
Graeme D. Ford
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
A. Jane Owen
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
Brian Tuckley
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
Malcolm Anderson
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
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Summary

A quantitative method for rapid routine analysis of individual free fatty acids (FFA) in milk was developed. Lipid was extracted from milk in ether and FFA were recovered by shaking the extract with anion exchange resin Amberlyst 26. The resin-bound FFA were methylated directly and the individual acids quantified, using internal standards, by gas-liquid chromatography. The properties of the resin were measured. The validity of the method was established by extraction of FFA mixtures and milk. Individual acids were, on average, found to be within 6% of the actual concentration present in the mixture. An average coefficient of variation of 4·3% was achieved for the major individual fatty acids on repeated extraction of a single milk sample.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 3 , August 1983 , pp. 321 - 329
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

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