Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-30T23:06:19.678Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of polymorphisms in the leptin, leptin receptor and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition

Published online by Cambridge University Press:  30 November 2011

Maria Glantz ,
Helena Lindmark Månsson ,
Hans Stålhammar  and
Marie Paulsson
Maria Glantz*
Affiliation:
Department of Food Technology, Engineering and Nutrition, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
Helena Lindmark Månsson
Affiliation:
Department of Food Technology, Engineering and Nutrition, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden Swedish Dairy Association, Ideon Science Park, SE-223 70 Lund, Sweden
Hans Stålhammar
Affiliation:
VikingGenetics, P.O. Box 64, SE-532 21 Skara, Sweden
Marie Paulsson
Affiliation:
Department of Food Technology, Engineering and Nutrition, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The relations between cow genetics and milk composition have gained a lot of attention during the past years, however, generally only a few compositional traits have been examined. The aim of this study was to determine if polymorphisms in the leptin (LEP), leptin receptor (LEPR) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes as well as genetic polymorphism of β-casein (β-CN), κ-CN and β-lactoglobulin (β-LG) impact several bovine milk composition traits. Individual milk samples from the Swedish Red and Swedish Holstein breeds were analyzed for components in the protein, lipid, carbohydrate and mineral profiles. Cow alleles were determined on the following SNP: A1457G, A252T, A59V and C963T on the LEP gene, T945M on the LEPR gene and Nt984+8(A-G) on the DGAT1 gene. Additionally, genetic variants of β-CN, κ-CN and β-LG were determined. For both the breeds, the same tendency of minor allele frequency was found for all SNPs and protein genes, except on LEPA1457G and LEPC963T. This study indicated significant (P<0·05) associations between the studied SNPs and several compositional parameters. Protein content was influenced by LEPA1457G (G>A) and LEPC963T (T>C), whereas total Ca, ionic Ca concentration and milk pH were affected by LEPA1457G, LEPA59V, LEPC963T and LEPRT945M. However, yields of milk, protein, CN, lactose, total Ca and P were mainly affected by β-CN (A2>A1) and κ-CN (A>B>E). β-LG was mainly associated with whey protein yield and ionic Ca concentration (A>B). Thus, this study shows possibilities of using these polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters.

Keywords

Dairy cattleleptinleptin receptoracyl-CoA:diacylglycerol acyltransferase 1 (DGAT1)milk protein polymorphismmilk composition
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 1 , February 2012 , pp. 110 - 118
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aleandri, R, Buttazzoni, LG, Schneider, JC, Caroli, A & Davoli, R 1990 The effects of milk protein polymorphisms in milk components and cheese-producing ability. Journal of Dairy Science 73 241–255CrossRefGoogle Scholar
Amenu, B & Deeth, HC 2007 The impact of milk composition on cheedar cheese manufacture. Australian Journal of Dairy Technology 62 171–184Google Scholar
Auldist, MJ, Walsh, BJ & Thomson, NA 1998 Seasonal and lactational influences on bovine milk composition in New Zealand. Journal of Dairy Research 65 401–411CrossRefGoogle ScholarPubMed
Banos, G, Woolliams, JA, Woodward, BW, Forbes, AB & Coffey, MP 2008 Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) gene loci on milk production, feed, and body energy traits of UK dairy cows. Journal of Dairy Science 91 3190–3200CrossRefGoogle ScholarPubMed
Berry, DP, Howard, D, O'Boyle, PO, Waters, S, Kearney, JF & McCabe, M 2010 Associations between K232A polymorphism in the diacylglycerol-O-transferase 1 (DGAT1) gene and performance in Irish Holstein-Friesian dairy cattle. Irish Journal of Agricultural and Food Research 49 1–9Google Scholar
Bobe, G, Beitz, DC, Freeman, AE & Lindberg, GL 1999 Effect of milk protein genotypes on milk protein composition and its genetic parameter estimates. Journal of Dairy Science 82 2797–2804CrossRefGoogle ScholarPubMed
Bovenhuis, H, van Arendonk, JAM & Korver, S 1992 Associations between milk protein polymorphisms and milk production traits. Journal of Dairy Science 75 2549–2559CrossRefGoogle ScholarPubMed
Buchanan, FC, Van Kessel, AG, Waldner, C & Christensen, DA 2003 An association between a leptin single nucleotide polymorphism and milk and protein yield. Journal of Dairy Science 86 3164–3166CrossRefGoogle ScholarPubMed
Buchberger, J & Dovc, P 2000 Lactoprotein genetic variants in cattle and cheese making ability. Food Technology and Biotechnology 38 91–98Google Scholar
Cases, S, Smith, S, Zheng, Y, Myers, H, Lear, S, Sande, E, Novak, S, Collins, C, Welch, C, Lusis, A, Erickson, S & Farese, RJ 1998 Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proceedings of the National Academy of Sciences of the United States of America 95 13018–13023CrossRefGoogle ScholarPubMed
Chebel, RC, Susca, F & Santos, JEP 2008 Leptin genotype is associated with lactation perfomance and health of Holstein cows. Journal of Dairy Science 91 2893–2900CrossRefGoogle Scholar
Conte, G, Mele, M, Chessa, S, Castiglioni, B, Serra, A, Pagnacco, G & Secchiari, P 2010 Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle. Journal of Dairy Science 93 753–763CrossRefGoogle ScholarPubMed
da Silva, MVGB, Sonstegard, TS, Thallman, RM, Connor, EE, Schnabel, RD & Van Tassell, CP 2010 Characterization of DGAT1 allelic effects in a sample of North American Holstein cattle. Animal Biotechnology 21 88–99CrossRefGoogle Scholar
Fruhbeck, G, Jebb, SA & Prentice, AM 1998 Leptin: physiology and pathophysiology. Clinical Physiology 18 399–419CrossRefGoogle ScholarPubMed
Glantz, M, Devold, TG, Vegarud, GE, Lindmark Månsson, H, Stålhammar, H & Paulsson, M 2010 Importance of casein micelle size and milk composition for milk gelation. Journal of Dairy Science 93 1444–1451CrossRefGoogle ScholarPubMed
Glantz, M, Lindmark Månsson, H, Stålhammar, H, Bårström, L-O, Fröjelin, M, Knutsson, A, Teluk, C & Paulsson, M 2009 Effects of animal selection on milk composition and processability. Journal of Dairy Science 92 4589–4603CrossRefGoogle ScholarPubMed
Glantz, M, Lindmark Månsson, H, Stålhammar, H & Paulsson, M 2011 Effect of polymorphisms in the leptin, leptin receptor, and acyl-coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on cheese characteristics. Journal of Dairy Science 94 3295–3304CrossRefGoogle ScholarPubMed
Grisart, B, Coppieters, W, Farnir, F, Karim, L, Ford, C, Berzi, P, Cambisano, N, Mni, M, Reid, S, Simon, P, Spelman, R, Georges, M & Snell, R 2002 Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research 12 222–231CrossRefGoogle ScholarPubMed
Hallén, E, Allmere, T, Näslund, J, Andrén, A & Lundén, A 2007 Effect of genetic polymorphism of milk proteins on rheology of chymosin-induced milk gels. International Dairy Journal 17 791–799CrossRefGoogle Scholar
Hallén, E, Wedholm, A, Andrén, A & Lundén, A 2008 Effect of beta-casein, kappa-casein and beta-lactoglobulin genotypes on concentration of milk protein variants. Journal of Animal Breeding and Genetics 125 119–129CrossRefGoogle ScholarPubMed
Heck, JLM, Schennink, A, van Valenberg, HJF, Bovenhuis, H, Visker, MHPW, van Arendonk, JAM & van Hooijdonk, ACM 2009 Effects on milk protein variants on the protein composition of bovine milk. Journal of Dairy Science 92 1192–1202CrossRefGoogle ScholarPubMed
Ikonen, T, Ahlfors, K, Kempe, R, Ojala, M & Ruottinen, O 1999a Genetic parameters for the milk coagulation properties and prevalence of noncoagulating milk in Finnish dairy cows. Journal of Dairy Science 82 205–214CrossRefGoogle ScholarPubMed
Ikonen, T, Ojala, M & Ruottinen, O 1999b Associations between milk protein polymorphism and first lactation milk production traits in Finnish Ayrshire cows. Journal of Dairy Science 82 1026–1033CrossRefGoogle ScholarPubMed
Ikonen, T, Ojala, M & Syväoja, EL 1997 Effects of composite casein and beta-lactoglobulin genotypes on renneting properties and composition of bovine milk by assuming an animal model. Agricultural and Food Science Finland 6 283–294CrossRefGoogle Scholar
Kaminski, S, Brym, P, Rusc, A, Wójcik, E, Ahman, A & Mägi, R 2006 Associations between milk performance traits in Holstein cows and 16 candidate SNPs identified by arrayed primer extension (apex) microarray. Animal Biotechnology 17 1–11CrossRefGoogle ScholarPubMed
Kaupe, B, Brandt, H, Prinzenberg, E-M & Erhardt, G 2007 Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction, and productive lifespan in German Holstein cattle. Journal of Animal Science 85 11–21CrossRefGoogle ScholarPubMed
Kay, JK, Weber, WJ, Moore, CE, Bauman, DE, Hansen, LB, Chester-Jones, H, Crooker, BA & Baumgard, LH 2005 Effects of week of lactation and genetic selection for milk yield on milk fatty acid composition in Holstein cows. Journal of Dairy Science 88 3886–3893CrossRefGoogle ScholarPubMed
Kgwatalala, PM, Ibeagha-Awemu, EM, Mustafa, AF & Zhao, X 2009 Stearoyl-CoA desaturase 1 genotype and stage of lactation influences milk fatty acid composition of Canadian Holstein cows. Animal Genetics 40 609–615CrossRefGoogle ScholarPubMed
Komisarek, J 2010 Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Animal Science Papers and Reports 28 133–141Google Scholar
Komisarek, J & Dorynek, Z 2006 The relationship between the T945M single nucleotide polymorphism in the leptin receptor gene (LEPR) and milk production traits in Jersey cows. Animal Science Papers and Reports 24 271–277Google Scholar
Komisarek, J, Szyda, J, Michalak, A & Dorynek, Z 2005 Impact of leptin gene polymorphisms on breeding value for milk production traits in cattle. Journal of Animal and Feed Sciences 14 491–500CrossRefGoogle Scholar
Kulig, H, Kmiec, M, Kowalewska-Luczak, I & Andziak, G 2009 Effect of leptin gene polymorphisms on milk production traits of Jersey cows. Turkish Journal of Veterinary and Animal Sciences 33 143–146Google Scholar
Kulig, H, Kmiec, M & Wojdak-Maksymiec, K 2010 Associations between leptin gene polymorphisms and somatic cell count in milk of Jersey cows. Acta Veterinaria Brno 79 237–242CrossRefGoogle Scholar
Liefers, SC, te Pas, MFW, Veerkamp, RF & van der Lende, T 2002 Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. Journal of Dairy Science 85 1633–1638CrossRefGoogle ScholarPubMed
Liefers, SC, Veerkamp, RF, te Pas, MFW, Delavaud, C, Chilliard, Y & van der Lende, T 2004 A missense mutation in the bovine leptin receptor gene is associated with leptin concentrations during late pregnancy. Animal Genetics 35 138–141CrossRefGoogle ScholarPubMed
Liefers, SC, Veerkamp, RF, Te Pas, MFW, Delavaud, C, Chilliard, Y, Platje, M & van der Lende, T 2005 Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Animal Genetics 36 111–118CrossRefGoogle ScholarPubMed
Lien, S, Kantanen, J, Olsaker, I, Holm, L-E, Eytorsdottir, E, Sandberg, K, Dalsgard, B & Adalsteinsson, S 1999 Comparison of milk protein allele frequencies in Nordic cattle breeds. Animal Genetics 30 85–91CrossRefGoogle ScholarPubMed
Lundén, A, Nilsson, M & Janson, L 1997 Marked effect of beta-lactoglobulin polymorphism on the ratio of casein to total protein in milk. Journal of Dairy Science 80 2996–3005CrossRefGoogle ScholarPubMed
Näslund, J, Fikse, WF, Pielberg, GR & Lundén, A 2008 Frequency and effect of the bovine acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal of Dairy Science 91 2127–2134CrossRefGoogle ScholarPubMed
Ng-Kwai-Hang, KF 1998 Genetic polymorphism of milk proteins: relationships with production traits, milk composition and technological properties. Canadian Journal of Animal Science 78 131–147Google Scholar
Nordic Cattle Genetic Evaluation 2011 Routine evaluation – milk traits. http://www.nordicebv.info/Routine+evaluation/Milk+traits/Milk+Traits.htm. Accessed Feb. 21, 2011Google Scholar
Schennink, A, Heck, JML, Bovenhuis, H, Visker, MHPW, van Valenberg, HJF & van Arendonk, JAM 2008 Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl CoA: diacylglycerol acyltransferase 1 (DGAT1). Journal of Dairy Science 91 2135–2143CrossRefGoogle ScholarPubMed
Schennink, A, Stoop, WM, Visker, MHPW, Heck, JML, Bovenhuis, H, van der Poel, JJ, van Valenberg, HJF & van Arendonk, JAM 2007 DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Animal Genetics 38 467–473CrossRefGoogle ScholarPubMed
Signorelli, F, Orrù, L, Napolitano, F, De Matteis, G, Scatà, MC, Catillo, G, Marchitelli, C & Moioli, B 2009 Exploring polymorphisms and effects on milk traits of the DGAT1, SCD1 and GHR genes in four cattle breeds. Livestock Science 125 74–79CrossRefGoogle Scholar
Spelman, RJ, Ford, CA, McElhinney, P, Gregory, GC & Snell, RG 2002 Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science 85 3514–3517CrossRefGoogle ScholarPubMed
Sun, D, Jia, J, Ma, Y, Zhang, Y, Wang, Y, Yu, Y & Zhang, Y 2009 Effects of DGAT1 and GHR on milk yield and milk composition in the Chinese dairy population. Animal Genetics 40 997–1000CrossRefGoogle ScholarPubMed
Swedish Dairy Association 2009 Dairy Statistics. Stockholm, Sweden: Swedish Dairy AssociationGoogle Scholar
Tellam, RL 2007 Capturing benefits from the bovine genome sequence. Australian Journal of Experimental Agriculture 47 1039–1050CrossRefGoogle Scholar
Thaller, G, Krämer, W, Winter, A, Kaupe, B, Erhardt, G & Fries, R 2003 Effects of DGAT1 variants on milk production traits in German cattle breeds. Journal of Animal Science 81 1911–1918CrossRefGoogle ScholarPubMed
Walstra, P & Jenness, R 1984 Dairy Chemistry and Physics. Hoboken, NJ: US John Wiley & Sons Inc.Google Scholar
Wedholm, A, Hallén, E, Larsen, LB, Lindmark Månsson, H, Karlsson, AH & Allmere, T 2006a Comparison of milk protein composition in a Swedish and a Danish dairy herd using reversed phase HPLC. Acta Agriculturae Scandinavica Section A 56 8–15CrossRefGoogle Scholar
Wedholm, A, Larsen, LB, Lindmark Månsson, H, Karlsson, AH & Andrén, A 2006b Effect of protein composition and the cheese-making properties of milk from individual dairy cows. Journal of Dairy Science 89 3296–3305CrossRefGoogle ScholarPubMed