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Dominance of the genus Polaromonas in the microbial ecology of an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating dairy processing wastewater under varying aeration rates

Published online by Cambridge University Press:  08 August 2018

Beatriz Gil-Pulido ,
Emma Tarpey ,
William Finnegan ,
Xinmin Zhan ,
Alan DW Dobson  and
Niall O'Leary
Beatriz Gil-Pulido
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
Emma Tarpey
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
William Finnegan
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
Xinmin Zhan
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
Alan DW Dobson
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
Niall O'Leary*
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
*
*For correspondence; e-mail: [email protected]
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Abstract

In this Research Communication we investigate potential correlations between key bacterial groups and nutrient removal efficiency in an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating synthetic dairy processing wastewater. Reactor aeration rates of 0·6 and 0·4 litre per minute (LPM) were applied to an 8 l laboratory scale system and the relative impacts on IASBR microbial community structure and orthophosphate (PO4-P) and ammonium (NH4-N) removal efficiencies compared. Aeration at 0·6 LPM over several sludge retention times (SRTs) resulted in approximately 92% removal efficiencies for both PO4-P and NH4-N. Biomass samples subjected to next-generation sequencing (NGS), 16S rRNA profiling revealed a concomitant enrichment of Polaromonas under 0·6 LPM conditions, up to ~50% relative abundance within the reactor biomass. The subsequent shift in reactor aeration to 0·4 LPM, over a period of 3 SRTs, resulted in markedly reduced nutrient removal efficiencies for PO4-P (50%) and NH4-N (45%). An 85·7% reduction in the genus level relative abundance of Polaromonas was observed under 0·4 LPM aeration conditions over the same period.

Keywords

Dairy wastewaterbioremediationnutrient removal
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 3 , August 2018 , pp. 388 - 390
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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References

APHA 2005 Standard methods for the examination of water and waste water, 21st edn. American Public Health Association, Washington, DCGoogle Scholar
Bragg, L, Stone, G, Imelfort, M, Hugenholtz, P & Tyson, GW 2012 Fast, accurate error-correction of amplicon pyrosequences using Acacia. Nature Methods 9 425–426Google Scholar
Caporaso, JG, Kuczynski, J, Stombaugh, J, Bittinger, K, Bushman, FD, Costello, EK, Fierer, N, Peña, AG, Goodrich, JK, Gordon, JI & Huttley, GA 2010 QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7 335–336Google Scholar
Demirel, B, Yenigun, O & Onay, TT 2005 Anaerobic treatment of dairy wastewaters: a review. Process Biochemistry 40 2583–2595Google Scholar
Ferrera, I & Sánchez, O 2016. Insights into microbial diversity in wastewater treatment systems: how far have we come? Biotechnology Advances 34 790–802Google Scholar
Ge, H, Batstone, DJ & Keller, J 2015 Biological phosphorus removal from abattoir wastewater at very short sludge ages mediated by novel PAO clade Comamonadaceae. Water Research, 69 173–182Google Scholar
He, S, Ding, LL, Xu, K, Geng, JJ & Ren, HQ 2016 Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge. Bioresource Technology 211 494–501Google Scholar
Pan, M, Henry, LG, Liu, R, Huang, X & Zhan, X 2013 Nitrogen removal from slaughterhouse wastewater through partial nitrification followed by denitrification in intermittently aerated sequencing batch reactors at 11 °C. Environmental Technology 35 470–477Google Scholar
Weissbrodt, DG, Shani, N & Holliger, C 2014 Linking bacterial population dynamics and nutrient removal in the granular sludge biofilm ecosystem engineered for wastewater treatment. FEMS Microbiology Ecology 88 579–595Google Scholar
Willems, A 2014 The family Comamonadaceae. In Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (eds) The Prokaryotes, pp. 777–851. Springer Berlin, HeidelbergGoogle Scholar