Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-20T07:23:44.847Z Has data issue: false hasContentIssue false
  • English
  • Français

Association between the GHR, GHRHR and IGF1 gene polymorphisms and milk coagulation properties in Sarda sheep

Published online by Cambridge University Press:  10 July 2019

Maria L. Dettori ,
Michele Pazzola ,
Emanuela Pira ,
Giorgia Stocco  and
Giuseppe M. Vacca
Maria L. Dettori*
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Michele Pazzola
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Emanuela Pira
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Giorgia Stocco
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Giuseppe M. Vacca
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
*
Author for correspondence: Maria L. Dettori, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

We investigated whether variation of the sheep Growth Hormone Receptor (GHR), Growth Hormone Releasing Hormone Receptor (GHRHR) and Insulin-Like Growth Factor 1 (IGF1) genes were associated with milk coagulation properties (MCP) in sheep. The GHR, GHRHR and IGF1 genes are part of the GH system, which is known to modulate metabolism, growth and reproduction as well as mammogenesis and galactopoiesis in dairy species. A total of 380 dairy Sarda sheep were genotyped for 36 SNPs mapping to these three genes. Traditional MCP were measured as rennet coagulation time (RCT), curd-firming time (k20) and curd firmness at 30 m (a30). Modeling of curd firming over time (CFt) was based on a 60 m lactodynamographic test, generating a total of 240 records of curd firmness (mm) for each milk sample. The model parameters obtained included: the rennet coagulation time as a result of modeling all data available (RCTeq, min); the asymptotic potential value of curd firmness (CFP, mm) at an infinite time; the CF instant rate constant (kCF, %/min); the syneresis instant rate constant (kSR, %/min); the maximum value of CF (CFmax, mm) and the time at achievement of CFmax (tmax, min). Statistical analysis revealed that variation of the GHR gene was significantly associated with RCT, kSR and CFP (P < 0.05). No other significant associations were detected. These findings may be useful for the dairy industry, as well as for selection programs.

Keywords

GHRGHRHRIGF1milk coagulation propertiessheep milk
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 3 , August 2019 , pp. 331 - 336
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adams, TE (1995) Differential expression of growth hormone receptor messenger RNA from a second promoter. Molecular and Cellular Endocrinology 108, 23–33.Google Scholar
Adams, TE, Baker, L, Fiddes, RJ and Brandon, MR (1990) The sheep growth hormone receptor: molecular cloning and ontogeny of mRNA expression in the liver. Molecular and Cellular Endocrinology 73, 135–145.Google Scholar
Akers, MR (2017) A 100-year review: mammary development and lactation. Journal of Dairy Science 100, 10332–10352.Google Scholar
Banos, G, Woolliams, JA, Woodward, BW, Forbes, AB and Coffey, MP (2008) Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) gene loci on milk production, feed, and body energy traits of UK dairy cows. Journal of Dairy Science 91, 3190–3200.Google Scholar
Barrett, JC, Fry, B, Maller, J and Daly, MJ (2005) Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics (Oxford, England) 21, 263–265.Google Scholar
Bergan-Roller, HE and Sheridan, MA (2018) The growth hormone signaling system: insights into coordinating the anabolic and catabolic actions of growth hormone. General and Comparative Endocrinology 258, 119–133.Google Scholar
Bittante, G (2011) Modeling rennet coagulation time and curd firmness of milk. Journal of Dairy Science 94, 5821–5832.Google Scholar
Bittante, G, Penasa, M and Cecchinato, A (2012) Invited review: genetics and modeling of milk coagulation properties. Journal of Dairy Science 95, 6843–6870.Google Scholar
Bittante, G, Contiero, B and Cecchinato, A (2013) Prolonged observation and modelling of milk coagulation, curd firming, and syneresis. International Dairy Journal 29, 115–123.Google Scholar
Bittante, G, Pellattiero, E, Malchiodi, F, Cipolat-Gotet, C, Pazzola, M, Vacca, GM, Schiavon, S and Cecchinato, A (2014) Quality traits and modeling of coagulation, curd firming, and syneresis of sheep milk of Alpine breeds fed diets supplemented with rumen protected conjugated fatty acid. Journal of Dairy Science 97, 4018–4028.Google Scholar
Carta, A, Casu, S and Salaris, S (2009) Invited review: current state of genetic improvement in dairy sheep. Journal of Dairy Science 92, 5814–5833.Google Scholar
Ceriotti, G, Chiatti, F, Bolla, P, Martini, M and Caroli, A (2005) Genetic variability of the ovine αs1-casein. Italian Journal Animal Science 4, 64–66.Google Scholar
Chaves, VE, Frasson, D and Kawashita, NH (2011) Several agents and pathways regulate lipolysis in adipocytes. Biochimie 93, 1631–1640.Google Scholar
Cipolat-Gotet, C, Cecchinato, A, Pazzola, M, Dettori, ML, Bittante, G and Vacca, GM (2016) Potential influence of herd and animal factors on the yield of cheese and recovery of components from Sarda sheep milk, as determined by a laboratory bench-top model cheese-making. International Dairy Journal 63, 8–17.Google Scholar
Cipolat-Gotet, C, Pazzola, M, Ferragina, A, Cecchinato, A, Dettori, ML and Vacca, GM (2018) Technical note: improving modeling of coagulation, curd firming and syneresis of sheep milk. Journal of Dairy Science 101, 5832–5837.Google Scholar
Dettori, ML, Pazzola, M, Pira, E, Paschino, P and Vacca, GM (2015) The sheep growth hormone gene polymorphism and its effects on milk traits. Journal of Dairy Research 82, 169–176.Google Scholar
Dettori, ML, Pazzola, M, Paschino, P, Amills, M and Vacca, GM (2018) Association between the GHR, GHRHR and IGF1 gene polymorphisms and milk yield and quality traits in Sarda sheep. Journal of Dairy Science 101, 9978–986. https://doi.org/10.3168/jds.2018-14914.Google Scholar
FAOSTAT (2016) Statistical Database of the Food and Agriculture Organization of the United Nations. FAOSTAT, Rome, Italy. Available at http://www.faostat.fao.org (Accessed 3 May 2018).Google Scholar
Gabriel, SB, Schaffner, SF, Nguyen, H, Moore, JM, Roy, J, Blumenstiel, B, Higgins, J, DeFelice, M, Lochner, A, Faggart, M, Liu-Cordero, SN, Rotimi, C, Adeyemo, A, Cooper, R, Ward, R, Lander, ES, Daly, MJ and Altshuler, D (2002) The structure of haplotype blocks in the human genome. Science 296, 2225–2229.Google Scholar
Herington, AC and Lobie, PE (2012) Signal transduction mechanisms underlying growth hormone receptor action. Open Endocrinology Journal 6, 13–21.Google Scholar
Jiang, H and Lucy, MC (2001) Variants of the 5′-untranslated region of the bovine growth hormone receptor mRNA: isolation, expression and effects on translational efficiency. Gene 265, 45–53.Google Scholar
Laviola, L, Natalicchio, A and Giorgino, F (2007) The IGF-I signaling pathway. Current Pharmaceutical Design 13, 663–669.Google Scholar
McMahon, DJ and Brown, RJ (1982) Evaluation of Formagraph for comparing rennet solutions. Journal of Dairy Science 65, 1639–1642.Google Scholar
Noce, A, Pazzola, M, Dettori, ML, Amills, M, Castelló, A, Cecchinato, A, Bittante, G and Vacca, GM (2016) Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep. Animal Genetics 47 717–726.Google Scholar
Pang, ALY and Chan, WY (2010) Chapter 22—molecular basis of diseases of the endocrine system. In Coleman, WB and Tsongalis, GJ (eds), Essential Concepts in Molecular Pathology. San Diego, USA: Academic Press, pp. 289–307.Google Scholar
Pazzola, M, Dettori, ML, Cipolat-Gotet, C, Cecchinato, A, Bittante, G and Vacca, GM (2014) Phenotypic factors affecting coagulation properties of milk from Sarda ewes. Journal of Dairy Science 97, 7247–7257.Google Scholar
Pazzola, M, Stocco, G, Dettori, ML, Cipolat-Gotet, C, Bittante, G and Vacca, GM (2018) Modeling of coagulation, curd firming, and syneresis of goat milk. Journal of Dairy Science 101, 7027–7039.Google Scholar
Stocco, G, Cipolat-Gotet, C, Bobbo, T, Cecchinato, A and Bittante, G (2017) Breed of cow and herd productivity affect milk composition and modeling of coagulation, curd firming and syneresis. Journal of Dairy Science 100, 129–145.Google Scholar
Vacca, GM, Dettori, ML, Balia, F, Luridiana, S, Mura, MC, Carcangiu, V and Pazzola, M (2013) Sequence polymorphism at the growth hormone GH1/GH2-N and GH2-Z gene copies and their relationship with dairy traits in domestic sheep (Ovis aries). Molecular Biology Reports 40, 5285–5294.Google Scholar
Vacca, GM, Pazzola, M, Dettori, ML, Pira, E, Malchiodi, F, Cipolat-Gotet, C, Cecchinato, A and Bittante, G (2015) Modeling of coagulation, curd firming, and syneresis of milk from Sarda ewes. Journal of Dairy Science 98, 2245–2259.Google Scholar
Viitala, S, Szyda, J, Blott, S, Schulman, N, Lidauer, M, MakiTanila, A, Georges, M and Vilkki, JJ (2006) The role of the bovine growth hormone receptor and prolactin receptor genes in milk, fat and protein production in Finnish Ayrshire dairy cattle. Genetics 173, 2151–2164.Google Scholar