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31P-nuclear magnetic resonance study of milk fractions

Published online by Cambridge University Press:  01 June 2009

Magnus Wahlgren ,
Torbjörn Drakenberg ,
Hans J. Vogel  and
Petr Dejmek
Magnus Wahlgren
Affiliation:
Department of Food Technology, University of Lund, Box 124, S-221 00 Lund, Sweden
Torbjörn Drakenberg
Affiliation:
Department of Physical Chemistry 2, University of Lund, Box 124, S-221 00 Lund, Sweden
Hans J. Vogel
Affiliation:
Department of Physical Chemistry 2, University of Lund, Box 124, S-221 00 Lund, Sweden
Petr Dejmek
Affiliation:
Department of Food Technology, University of Lund, Box 124, S-221 00 Lund, Sweden
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Summary

Milk serum, whey and milk ultrafiltrate were examined by 31P nuclear magnetic resonance (31P-NMR). About 20 phosphorylated milk constituents gave rise to resonances in the spectra. Most of these have been assigned to such well-known milk constituents as inorganic phosphate, N-acetylglucosamine-1-phosphate and glycerophosphorylcholine. Resonances from previously unknown constituents such as phosphocreatine were also observed. When the pH-dependence of inorganic phosphate, N-acetylglucosamine-1 -phosphate, glycerophosphorylcholine and gly-cerophosphorylethanolamine was examined it was observed that the resonance of inorganic phosphate overlapped that of N-acetylglucosamine-1-phosphate around neutral pH. This is the most probable explanation as to why this constituent was not observed in earlier 31P-NMR studies on milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 4 , November 1986 , pp. 539 - 545
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

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