A study on the use of quebracho tannins as chemical additives was carried out at the Spanish Council
for Scientific Research, León, Spain during 1998 by conducting two experiments. In the first
experiment, three ruminally cannulated ewes were used to determine in situ degradability of soya bean
meals (SBM) treated with different doses of quebracho tannin. Samples were prepared by spraying
100 g SBM with 100 ml distilled water containing 0, 1, 5, 10, 15 or 25 g of commercial quebracho
powder (S0, SQ1, SQ2, SQ3, SQ4 and SQ5, respectively). Intestinal digestibility of non-degradable protein
was estimated in vitro. The rapidly degradable protein fraction of all quebracho treated soya bean
meals was different (P<0·05) from the non-treated SBM (S0: 0·154), with values ranging from 0·032
to 0·133. SQ4 and SQ5 showed lower fractional rates of degradation of the protein than S0 (0·042 and
0·046 v. 0·082, respectively). By contrast, in vitro digestibility of the non-degradable protein was not
significantly affected by the treatments imposed, with the exception of treatment with the highest dose
of quebracho tannin in which intestinal digestion was reduced (0·939 v. 0·826 for S0 and SQ5,
respectively. P<0·05). In the second experiment, ten ruminally cannulated ewes were used to examine
the effect of a daily dosing of quebracho tannin into the rumen on the degradation of the quebracho-treated SBM. Long-term dosing of quebracho tannin did not increase the ability of the rumen
microbes to degrade tannin treated SBM. It was therefore concluded that quebracho tannins could
be used as chemical additives for improving the digestive utilization of protein-rich feeds in sheep.