Published online by Cambridge University Press: 27 March 2009
A variety of chemical reagents and treatment methods have been tested for their potential to enhance the digestibility of crop residues. The most universally used chemicals for animal experimentation in residue treatment are sodium or ammonium hydroxide (Chandra & Jackson, 1970; Koers, Prokop & Klopfenstein, 1972; Klopfenstein, 1978; Arndt & Richardson, 1982). These chemical treatments usually improve digestibility of roughages by solubilizing hemicellulose, and increasing the extent and rate of cellulose and hemicellulose digestion (Klopfenstein, 1978). Delignification is not usually considered to be an important aspect of chemical treatment (Klopfenstein et al. 1972), and increases in digestion are usually attributed primarily to breaking of bonds between lignin and carbohydrates rather than lignin removal. The aromatic nuclei of the lignin molecule are quite susceptible to oxidative attack (Sarkenen & Ludwig, 1971), and crop residues may be significantly delignified by oxidative chemical reagents (Sullivan & Hershberger, 1959; Sherrod et al. 1978; Ben-Ghedalia, Shefet & Miron, 1980). Ozonation is a chemical treatment method which is known to oxidize lignin and disrupt the lignocellulose complex. Several researchers have successfully applied the delignifying capability of ozone to improve the in vitro digestibility of roughages (Weakley & Owens, 1975; Ben-Ghedalia & Miron, 1981; Tock et al. 1982). Very little, however, is known of the actual in vivo feeding value of crop residues treated with ozone. These studies were conducted to determine the effect of ozone treatment on the feeding value of sorghum stover.