Individual muscles, individual bones and fat depots from the non-carcass parts (head, foreshanks and tail) of 40 steers (chilled carcass weight 142–174 kg) were studied in an attempt to predict carcass composition more accurately than in an earlier study where the crude non-carcass parts, head, tongue, foreshanks and hindshanks were used.

The technique did not improve the accuracy of prediction of muscle or bone. Carcass fat, which was calculated by difference in the earlier study, was predicted more accurately using intermandibular fat weight (requiring 3–4 min to obtain) plus fat thickness at the 12th rib. However, the accuracy of this prediction (carcass fatness range 14·0–33·6%, residual standard deviation 1·98%) was no greater than that using chilled side weight plus fat thickness at the 12th rib.

Careful dissection and trimming of anatomical entities from the non-carcass parts therefore offered a slight advantage only in the prediction of carcass components over existing techniques.

Substitution of hot side weight for chilled side weight, and sacral crest fat thickness for fat thickness at the 12th rib did not lead to a loss in the accuracy of prediction of muscle, bone or fat.

The persistence under a close intermittent grazing regime of eight accessions of Stylosanthes guianensis, Stylosanthes macrocephala and Stylosanthes capitata in combination with Andropogon gayanus (cv. Planaltina) and Brachiaria decumbens (cv. Basilisk) was evaluated in small plots on a low fertility acid oxisol in the tropical savannah region of Brazil. Five accessions persisted for four seasons with both grasses, although legume yields were lower in the B. decumbens associations.

S. macrocephala CIAT 1582 (CPAC 139), S. capitata CIAT 1019 (CPAC 704) and CIAT 1097 (CPAC 706) were the most productive accessions at the end of the experiment. The three accessions S. guianensis cv. Cook, S. capitata CIAT 1315 (CPAC 707) and CIAT 1405 (CPAC 846), which failed to persist, were destroyed by anthracnose (Colletotrichum gloeosporioides). This disease remains the primary factor limiting the use of Stylosanthes in the region. The value of small-plot grazing experiments is briefly discussed.

Fifteen British Friesian steers were reared using grain and forages which had been grown on land which has had a long history of use for disposal of sewage sludges. The food consumption was recorded and samples of feedingstuffs analysed for Cd, Cr, Cu, Ni and Zn. The various components used in compounding the feedingstuffs were also analysed. After about 13 months the animals were slaughtered and samples of various organs and tissues obtained for analysis.

The majority of the Cd, Cr, Cu and Zn came from sources other than uptake into plants. The largest single contributor was the high phosphate mineral supplement. No animal health problem due to the feed regime was encountered. Concentrations of cadmium in liver and kidney were slightly higher than average. Muscle cadmium concentrations of Cr, Cu, Pb, Ni and Zn were within the range of published values.

Field experiments were conducted during the rainy seasons (June–October) of 1978 and 1979 in order to determine the effect of fertilizer N rates (0, 40, 80 and 120 kg N/ha) on yield and yield attributes of some new sorghum cultivars in northern India. Sorghum hybrid varieties yielded more than pure line varieties, hybrid CSH 5 outyielding all other cultivars including other hybrid varieties because of its greatest number of grains per panicle. Nitrogen application at 80 or 120 kg N/ha brought about significant improvement in number of grains per panicle and yield per ha.

Cultivar Χ N rate interactions were significant, in affecting the yield per ha and number of grains per panicle in 1978, whereas such differential response of sorghum cultivars to N rates did not occur in 1979. Sorghum hybrid varieties, even at lower N rates, produced identical yields to the pure line varieties supplied with higher N rates.

Two digestion trials were performed with sheep in order to assess the effect of the sulphur dioxide treatment on the digestibility of wheat straw in vivo. In the first trial, untreated and SO2-treated straw served as the major components of the diets. Sulphur dioxide partly solubilized the cell wall (CW) material of the straw, decreasing its content from 78·5 to 55·9%. The digestibility of the residual CW was increased from 49·2 to 77·8%, whereas the digestibility values of the cell-soluble fraction (CS) were unaffected by the treatment: 55·8 and 57·8% for the untreated and treated straw, respectively. Apparent digestibility of the organic matter (OM) was increased by the treatment from 46 to 65%.

In the second trial SO2-treated wheat straw was fed together with concentrates at a D.M. ratio of 30/70, and the digestibility of the OM and CW was determined.

CW and OM digestibilities of the treated straw were not affected by 70% concentrate in the diet and the respective values were 77·0 and 65·5%, very similar to those found in the first trial. The reason for the in vitro–in vivo discrepancy is discussed.

Live-weight gain of beef cattle grazing all-grass or grass-clover pastures, details of which have been reported previously, was significantly (P < 0·01) correlated with pasture growth rate, but not with pasture dry matter on offer (either total or green), or with dietary-energy concentration. From ideas generated from the relationship between live-weight gain and pasture growth rate, and using intake values measured from the number and size of prehension bites, it is shown how high live-weight gain for measured dietary-energy concentration could be explained by differential contribution to digestibility and voluntary intake from pasture new growth.

Isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), β-hydroxybutyrate dehydrogenase (β-OH-BDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) were histochemically located in the testes of buffaloes, goats and rams. The enzyme activities varied with the enzyme, species and cell type. The activities in the seminiferous tubules were correlated with the stages of seminiferous epithelial cycle (SEC). During this cycle, the activities in the Sertoli cells, spermatogonia and spermatocytes remained unaltered in contrast to those in the spermatids. The activities of SDH, ICDH and MDH were relatively greater in buffalo, while goat and ram resembled each other quite closely. ICDH and MDH preferred NADP to NAD. In the three species, the activities of ICDH, SDH and MDH generally followed an increasing order. G-6-PDH was greater in the interstitial tissue of buffalo than in goat and ram; the maximum activity of this enzyme in each species was found in the spermatogonia. In comparison with G-6-PDH, GDH was less evident in the interstitial tissue of buffalo and goat; Sertoli cells and spermatogonia also showed relatively less MDH activity whereas the other germ cells may have relatively less, similar or more, GDH activity depending on the species. β-OHBDH activity was similar in the interstitial tissue of the three species, but in the seminiferous tubule, the activity was less in goat. But for GDH and β-OH-BDH which could show different results, the activities of other enzymes generally decreased from spermatogonia through spermatocytes to spermatids but increased during spermiogenesis. In spermatozoa, the enzymes were observed only in the mid-piece. The possible physiological significance of the results is discussed in relation to different metabolic pathways.

Azospirillum brasilense was treated with nitrosoguanidine and five drug-resistant mutant strains isolated. The effects of acriflavin on pre- and post-irradiation with u.v. light and the level of antibiotic resistance were studied. Variations in factors were found between the strains. Inoculation of finger millet with A. brasilense and mutant strains led to significant increases in grain yield and nitrogenase activity compared with the uninoculated control, with significant strain x genotype interactions. Differential response of genotype and strain was noted on the protein and amino acid concentration of seeds.

Sixty-two genotypes of Trifolium repens (white clover) collected from latitudes 52° to 68° N and five selected genotypes of the cultivar S.184 were screened for shoot growth at low temperature and short daylengths in a controlled environment cabinet and in the field, and subjected to cold hardiness treatments ranging from – 2·5 to – 14·0 °C.

Considerable variation was discovered in several of the growth characteristics and in the frost tolerance of the genotypes. Genotypes exhibiting the highest rates of stolon extension were found in the more southerly latitudes. A high proportion of leaves produced during winter on the primary stolons of the selected cultivar plants subsequently died during the late spring at the upland field site, but several of the naturally occurring genotypes were able to retain more leaves per stolon in spring. Some genotypes appeared to maintain either short or long internodes throughout the spring in the field, whilst others produced relatively short internodes in early spring and long internodes later in the season, and vice versa. Only a small percentage of the genotypes possessed relatively long leaflets. A correlation was found between maximum leaflet length of the genotypes in early spring, and maximum leaflet length in late spring.

No relationship was found between frost tolerance and the growth of plants at low temperature. Frost sensitive and tolerant genotypes were found throughout the latitudinal and altitudinal ranges studied. Exposure to prevailing winds and possibly snow cover at the site of origin appeared to be important factors associated with the frost tolerance of T. repens plants.

A production system is defined which involves the production of lambs from crossbred ewes kept in a cool, temperate grassland environment at an altitude of 250–300 m. Within the production system, an investigation was carried out on the effect of the timing of weaning, pre-mating treatment (flushing) and concentrate feeding before lambing in a flock of 180 Border Leicester (♂) × Scottish Blackface (♀) ewes maintained for a production span of four lamb crops. Weaning treatment had no significant effects on changes in live weight or body condition score. Pre-mating treatment affected live weight and condition at mating. It did not affect the number of lambs born but had a significant effect on the subsequent birth weight of twin lambs in the 1st year it was applied but not thereafter. The timing of introduction and total quantity of concentrate feed affected the birth weight of lambs from ewes at their first lambing (24 months old) but not subsequently. There were no long-term effects of treatment on ewe survival or on the production characters measured. The results are discussed in relation to the seasonal patterns of grass supply and the normal limits of commercial practice. In this study, when grass and forage were in reasonable supply, pre-mating management and concentrate feeding were not critical to ewe performance. Only in circumstances of an unusual grass shortage and also in young ewes did these factors have significant effects on performance.

The analysis of plant competition experiments in which all possible pairs of p genotypes are grown in replacement series is discussed. Two methods of analyses, using models of de Wit (1960) and Gleeson & McGilchrist (1980), are compared. The two approaches, which are shown to be linked algebraically, complement each other in terms of agronomic interpretation.

The roles of leaves, stems and reproductive parts in 14CO2 fixation and its subsequent movement in winter oil-seed rape, cultivar Jet Neuf, were investigated in two field experiments carried out on the Wye College Farm. The relative importance of these organs changed with time. At early flowering the leaves were the most important photosynthetic organs (66·8% 14CO2 activity). Midway between flowering and maturity the stems became the major supplier of photosynthates, a role eventually taken over by the developing siliquae (pods).

These data stress the importance of keeping all photosynthetically active surfaces free from disease if efficient resource utilization and higher yields are to be achieved.

A replicated, long-term field drainage experiment on a clay soil was started in 1978 to investigate the effects of mole drainage on land that had been direct-drilled or ploughed. We here describe the background and the need for this work, details of the experiment and summarize the results for the first 2 years. The main factors causing point-to-point variability in the soil at the experimental site and their relative contributions were assessed. Variations in inherent soil properties were small, except for depth to calcareous clay. Site hydrology was also generally uniform, especially soil water content, and was little affected by the presence of subsoil drainage schemes dating from the 19th century. There were some variations in micro-relief. The drained and undrained plots were hydrologically separated. Equipment was installed to measure separately surface runoff, lateral flow at the bottom of the cultivated layer (the interflow) and deep drainage from the mole and pipe system (in the drained plots only). The design and performance of that equipment is described. In both years much of the undrained soil became saturated in winter, but a residual cultivation pan limited the effect of the mole drains in 1978–9. In 1979–80, after disrupting the pan, the water table in the moledrained plots was about 25 cm deeper than in the undrained plots, root growth was greater and yield of winter wheat 11% heavier. About 90% of water and nutrients draining from the drained plots were carried in the mole and pipe system. The results on water balance, nutrient losses, crop growth and yield are fully reported in companion papers (Harris et al. 1984; Ellis et al. 1984).

The use of phosphatidyl choline (PC) as a marker of protozoa in duodenal digesta was examined in sheep fed chaffed lucerne hay (Medicago sativa L.; 330–950 g organic matter/day) either once daily or hourly.

PC was present in rumen protozoa (N:PC-P = 185±6·4) but was not detected in rumen bacteria. Dietary intake of PC was less than 5% of the daily passage of PC from the stomach (7·49 mg PC-P/day). A comparison of the SA of PC-P in protozoa and in duodenal digesta of sheep given a continuous intraruminal infusion of 32P-orthophosphate showed that the proportion of dietary PC-P in duodenal digesta was negligible.

The limitations and validity of PC as a marker of protozoa in duodenal digesta are discussed. The daily passage of protozoal N from the stomach (1·38 ± 0·125 g/day) estimated with PC was not affected by frequency of feeding but protozoal N yield (4·96 ± 0·509 g/kg organic matter apparently digested) declined with increasing feed intake.

The SA of DNA-32P, RNA-32P and readily extractable phospholipid-32P (REPL-32P) in rumen protozoa, bacteria and duodenal digesta were similar, showing that the DNA, RNA and REPL-P in duodenal digesta were of microbial origin. A method is described for the estimation of bacterial REPL-P and bacterial N in duodenal digesta using DNA, RNA or DAPA as markers.

The calculated total microbial REPL-P (bacterial plus protozoal) in digesta using DNA or RNA as markers was in close agreement with values obtained by direct measurement. It was estimated that protozoa contributed 22 and 14% of microbial REPL-P and microbial N, respectively. Bacterial REPL-P and bacterial N values estimated with DAPA as the marker were higher than values obtained with DNA or RNA. These results are discussed with regard to the use of these markers.

Field experiments were conducted during the winter seasons of 1977–8 and 1978–9 at Agronomy Research Area of Haryana Agricultural University Farm, Hissar, to identify the visual plant characters that coincide with physiological maturity (PM) and 95% maximum grain dry weight (95% MGW) in wheat (Triticum aestivum L.) cultivars. The dry-weight accumulation data from three cultivars (WH-157, HD-2009 and UP-270) differing in many agronomic characters were fitted to polynomial regression equations to calculate time of PM and 95% MGW. The mean number of days from anthesis to 95% MGW and from anthesis to PM ranged from 31 to 36 and 38 to 43, respectively, depending upon cultivar. The time of 95% MGW preceded PM by about 7 days. Of the nine visual plant characters recorded during the grain-filling period, complete loss of green colour from the glumes occurred close to PM. Complete loss of green colour from the flag leaf most consistently coincided with the time of 95% MGW. Complete loss of green colour from the flag leaf could therefore be used as an indicator of the commencement of rapid decrease in grain dry weight and the final stage of grainfilling which ends when the glumes have lost the whole of their green colour.

Cell mass (dry matter per cell) and cell composition (concentrations of DNA, RNA, phospholipids, total N, a-dextran, diaminopimelic acid and 18 common amino acids) of rumen bacteria were measured at various times after feeding sheep chaffed lucerne hay (Medicago sativa L.) once daily. Cell composition was measured with sheep fed once hourly. Total DNA and RNA pool sizes in the rumen were also measured.

While cell composition was not affected by level of feed intake (700 g v. 1050 g dry matter/day), total DNA, RNA and D.M. pool sizes in the rumen increased with increasing feed intake. With sheep on the once daily feeding regimen relative rumen pool sizes in rumen digesta at various times after feeding were: RNA, 4 > 14 > 0 h; DNA, 4 and 14 > 0 h; D.M. 4 > 14 > 0 h. With the hourly feeding regimen pool sizes were similar to the averaged daily values for sheep fed once daily.

When sheep were fed once daily bacterial cell mass, DNA and phospholipid concentrations peaked at 12–14 h after feeding and subsequently decreased to the 0 h value. RNA concentration was maximal at about 4 h after feeding and declined to near the 0 h value at about 14 h. RNA concentrations in bacteria were highly correlated with gas production rates by whole rumen digesta. The ratio RNA:DNA was highest shortly after feeding, decreased to below the 0 h value at about 14 h and then increased to the 0 h value. The relative concentrations of a-dextran in bacteria were: 4 > 14 > 0 h. Cell composition witli sheep fed hourly tended to reflect the averaged daily values for sheep fed once daily.

These results are discussed with regard to changes in estimated fermentation rate and pool size of bacteria in the rumen. It is suggested that changes in average composition (DNA, RNA, total N and RNA: DNA ratio) of mixed rumen bacteria reflect changes in the average growth rate of the population.

Three cutting stages, the boot stage, 50% heading and the milk stage of grain, were imposed on eight barley varieties at four sites, two in each of two successive cropping seasons. Dry-matter and digestible yield increased significantly when harvest was delayed from the boot to the milk stage but crude-protein content and digestibility declined with advanced maturity. A similar pattern of response was obtained in most cases when varieties were examined individually. Variety 628 produced the highest yield, 8·34 t D.M./ha, while the most digestible variety was Athenais with 61·9% digestibility. In low-rainfall regions harvesting at the later stages of maturity is recommended, to take advantage of the higher D.M. yields.

The soil water regimes, flow paths of water and concentrations of nutrients in this water were measured for a clay soil growing winter wheat in 1978–9 and 1979–80. The soil was either drained with mole drains at 2 m spacing connected to plot drains 46 m apart or undrained. In the 1st year a compacted layer at about 20 cm depth caused a perched water table in the Ap horizon in both drainage treatments, and prevented the mole drains at 60 cm from affecting the water table. In 1979–80 after cultivation to disrupt the compacted layer, midway between the mole drains the depth to the winter water table was 20 cm greater than in undrained soil.

Surface flow, interflow at the depth of the plough layer and deep drainflow from mole and pipe drains responded rapidly to winter rainfall events. During both winters the mole and tile system removed most of the rainfall on the drained plots and the peaky hydrographs were typical of a mole system in a clay soil. In the undrained plots only a small proportion of the winter rainfall was accounted for in flow from the top 30 cm, and up to 75% of the water was able to percolate downwards possibly to below the barriers that separated the plots. Long-term water-balance studies indicated that a proportion of the water moving to depth in the undrained plots was probably entering the deep drainage system of the drained plots. As a result, the mole and pipe drainage system often removed more water than the rainfall input less evapotranspiration. This problem did not affect the depth to the water tables.

For each flow component concentrations of nitrate, ammonium, nitrous oxide, phosphorus, potassium and calcium were measured in the drainage water. Concentrations of nitrate-N from all drained plots were largest in autumn, being in the range 50–95 mg N/1, but then decreased to 1–5 mg N/1 by the end of March. Losses of nitrate-N were mainly through the mole drains and amounted to 43·6 and 59·7 kg N/ha in the 2 years. The quantities of nitrate-N lost in surface runoff or in flow in the cultivated layer were small on both treatments. Gaseous nitrous oxide, ammonium and phosphorus contents were very small. Potassium concentrations were somewhat larger, but not exceeding 3·5 mg/1. The calcium concentrations were in the range 40–210 mg/1. Concentrations of herbicides measured in November 1980 were negligible.

In the 2nd year water was taken up from a greater depth in the drained than in the undrained plots from April onwards. These results are discussed in relation to water supply to the crops at this site.

The results of phosphorus kinetic studies from 149 sheep used in previous studies of calcium and P metabolism have been analysed statistically, to provide information on the mechanism of control of P homoeostasis.

Whereas the rate of absorption of dietary P was increased in direct relation to P intake, the efficiency of absorption was decreased. Both the rate and efficiency of absorption, however, were increased in response to increased P demands. These results suggest that P may be absorbed by two processes: a passive one, related to P intake, and an active one, related to P demands.

Endogenous loss of P in the faeces was directly related to both P intake and P absorption but inversely related to P demands. Salivary secretion of P was directly related to P absorption and increased at a rate of 1 mg/day/kg body weight for each 1 mg/day/kg body weight increase in absorption. This finding supports the suggestion that the inevitable endogenous faecal loss of P, in animals fed exactly according to their P requirements, is not constant but varies in direct relation to the rate of P absorption, and hence to the P intake needed to supply these requirements.

Urinary loss of P was generally low, but high values were found in some animals and were associated with increased rates of P absorption and decreased rates of endogenous faecal loss of P. Possible reasons for these high rates of urinary P are discussed.

Increased P demands were met by a combination of an increase in P absorption and a decrease in endogenous faecal loss of P, and P retention was directly related to Ca retention.

The relevance of these results to the calculation of P requirements and to the control of P homoeostasis is discussed.

Grain yield of winter barley in the year preceding the experiment (1978) was relatively uniform over the site. In 1978–9 after the drainage treatments had been introduced, growth and yield of winter wheat were not affected by drainage, probably due to a compact layer at 20 cm that prevented the mole drains from controlling the water table (Harris et al. 1984).

In 1979–80 after disrupting this layer, root growth in undrained plots during the winter and spring was severely restricted by the presence of a water table 20 cm from the soil surface, although some root axes were able to grow down to 75–100 cm below the soil surface. The differences in root distribution patterns between drained and undrained plots disappeared after the water table declined in April. Uptake of nitrogen, phosphorus and potassium were less on undrained plots. Dry-matter production, leaf area index and peak number of tillers was also depressed, so that the yield of winter wheat was 0·74 t/ha greater on the drained treatment; the grain from the drained plots contained fewer impurities and weed seeds.