Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-25T06:08:49.094Z Has data issue: false hasContentIssue false
  • English
  • Français

Attempts at storage of sheep ova at low temperatures

Published online by Cambridge University Press:  27 March 2009

R. L. W. Averill  and
L. E. A. Rowson
R. L. W. Averill
Affiliation:
A.R.C. Unit of Reproductive Physiology and Biochemistry, University of Cambridge
L. E. A. Rowson
Affiliation:
A.R.C. Unit of Reproductive Physiology and Biochemistry, University of Cambridge
Get access Rights & Permissions [Opens in a new window]

Extract

Eighty-three fertilized sheep ova were stored at 0–8° C. for 6–72 hr. in sterile sheep serum or Ringer's solution and subsequently transferred to suitable recipient ewes in order to determine their viability following storage.

In Exp. 1, nine out of twelve ova stored in sheep serum, but none out of five ova stored in Ringer's solution, continued development after subjection to storage at 5–8° C. for 6–9 hr. Seven out of fifteen, none of nineteen, and none of nine ova were found developing in recipients after subjection to 5–8° C. in serum for 24, 48 and 72 hr., respectively.

In Exp. 2, lambs were born to recipients of four out of six ova stored for 24 hr. at 4·5–7° C, and of two out of five ova stored for 72 hr. at these temperatures. None of the recipients of twelve ova stored for 24, 48 and 72 hr. (four ova of each duration) at 0·4° C. produced live lambs.

In Exp. 3, none of thirty-four ova developed after subjection to freezing to −79° C. in 12·5% glycerol in serum.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 52 , Issue 3 , June 1959 , pp. 392 - 395
Copyright
Copyright © Cambridge University Press 1959

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Austin, C. R. (1956 a). J. Exp. Biol. 33, 338.Google Scholar
Austin, C. R. (1956 b). J. Exp. Biol. 33, 348.CrossRefGoogle Scholar
Averill, R. L. W. (1956). Dissertation on Fertility of the Ewe, Cambridge University.Google Scholar
Averill, R. L. W. (1958). J. Agric. Sci. 50, 27.CrossRefGoogle Scholar
Averill, R. L. W. & Rowson, L. E. A. (1958). J. Endocrin. 16, 326.Google Scholar
Chang, M. C. (1947). Nature, Lond., 159, 602.CrossRefGoogle Scholar
Chang, M. C. (1948 a). J. Gen. Physiol. 31, 385.Google Scholar
Chang, M. C. (1948 b). Proc. Soc. Exp. Biol, N.Y., 68, 680.Google Scholar
Chang, M. C. (1948 c). Nature, Lond., 161, 978.Google Scholar
Chang, M. C. (1950). Science, 111, 544.CrossRefGoogle Scholar
Chang, M. C. (1952). J. Exp. Zool. 121, 351.CrossRefGoogle Scholar
Chang, M. C. (1954). J. Exp. Zool. 124, 127.CrossRefGoogle Scholar
Deanesley, R. (1954). J. Endocrin. 11, 197.Google Scholar
Fekete, E. & Little, C. C. (1942). Cancer Res. 2, 525.Google Scholar
Marden, W. G. R. & Chang, M. C. (1952). Science, 115, 705.Google Scholar
Milovanov, V. K. (1934). Principles of A.I., Moscow.Google Scholar
Nicholas, J. S. (1933). Proc. Soc. exp. Biol., N.Y., 30, 111.CrossRefGoogle Scholar
Pincus, G. (1930). Proc. roy. Soc, Lond., B, 107, 132.Google Scholar
Pincus, G. (1949). Proc. Nat. Egg Transf. and Breeders Conf., San Antonio, Texas, 1, 18.Google Scholar
Smith, A. U. (1952). In Ciba Foundation Symposium: Mammalian Germ Cells, p. 217. London: Churchill.Google Scholar