Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-15T09:24:33.795Z Has data issue: false hasContentIssue false
  • English
  • Français

Functional adaptation of silk glands after administration of antibiotic to larvae of Philosamia ricini (Boisd)

Published online by Cambridge University Press:  19 September 2011

M. A. A. Eid ,
A. N. El-Nakkadi  and
M. A. Saleh
M. A. A. Eid*
Affiliation:
Professor of Economic Entomology, Faculty of Agriculture, Cairo University, Egypt
A. N. El-Nakkadi
Affiliation:
Professor of Biochemistry, Faculty of Agriculture, Cairo University, Egypt
M. A. Saleh
Affiliation:
Assistant Researcher, Plant Protection Institute, Ministry of Agriculture, Giza, Egypt
*
* To whom correspondence should be addressed.
Get access

Abstract

The effects of applying varying doses of an antibiotic to fifth instar larvae of Philosamia ricini on the silk glands were studied. Transaminases were increased by low doses, while RNA accumulation was reduced after administration of tetracycline. The levels of DNA showed little response to treating with antibiotic. However, the incorporation of amino acids into silk proteins was generally increased. The overall effects on the silk gland was reflected by its weight, cocoon weight, quantity of raw silk and quality of silk. All these indices were improved by administration of tetracycline.

Résumé

Les effets d'appliquer un antibiotic, aux larves du 5em stage, sur les glandes a soie furent etudies. Les transaminases augmenterent apris les petites doses, tandis que l'accumulation du RNA diminua apris l'administration du tetracycline. Les niveaux du DNA montrerent de faibles reactions au traitement arec l'antibiotique. L'incorporation des acides amines au proteine de la soie augmenta L'efficacite generale des glandes a soie fut refletee sur leur poids, le poids des cocons, la quantite et la qualite de la soie. Toutes ces indices augmenterent par l'administration du tetracycline.

Keywords

Philosamia ricinitetracyclinesilk gland enzymessilk gland RNAsilk gland DNAweight of silk glandweight of cocoonstrength of silk
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 10 , Issue 2 , April 1989 , pp. 139 - 143
Copyright
Copyright © ICIPE 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Agalykov, A., Kullyev, P., Mamedniyazov, O. and Karaev, K. (1974) Olivomycin effect of different RNA fractions during metamorphosis of the silkworm, Izv. Akd. Nauk. Turkm. SSR Biol. Nauk. 6, 16–19.Google Scholar
Agalykov, A., Kullyev, P., Mamedniyazov, O. and Zbraskii, I. (1975) The effect of antibiotics, actinomycin D and olivemycin on RNA biosynthesis and morphogenesis during metamorphosis of the silkworm Bombyx mori. Izv. Akad. Nauk. Turkm. SSR Ser. Biol. Nauk 12, 24–29.Google Scholar
Block, R., Durrum, E. and Zweig, G. (1958) A Manual of Paper Chromatography and Paper Electrophoresis. 2nd Ed., Academic Press, Inc. Publishers, New York.Google Scholar
Eid, M. and Salem, M. (1977) Nutritional studies on the silkworm Philosamia ricini Boisd. Bull. Fac. Agric., Cairo University, 28, 391–407.Google Scholar
El-Maasarawy, S. (1980) Studies on the haemolymph of Philosamia ricini Boisd. under different conditions. M.Sc. Thesis, Faculty of Agriculture, Cairo University.Google Scholar
Galimova, L., Mednikov, B. and Noskov, V. (1968) Streptomycin induced modification of sericins of the mulberry silkworms. Biokhimiya 33, 999–1003.Google Scholar
Kind, P. and King, E. (1954) Determination of alkaline phosphatase. J. Clin. Pathol. 7, 322.CrossRefGoogle Scholar
Kreysizg, E. (1970) International Mathematical Statistics Principles and Methods. Library of Congress Catalogue pp. 70–107.Google Scholar
Mednikov, B., Galimova, L. and Belozerskii, (1970) Patterns of translation mistakes in vivo and in vitro. Biokhimiya 35, 216–223.Google Scholar
Murihy, M., Shankaronarayan, M. and Sreenivasaya, M. (1954) Role of chloromycetin in the nutrition of the silkworm Bombyx mori. J. Sci. Indust. Res. (India) 13B, 331–335.Google Scholar
Reitman, S. and Frankel, S. (1957) Cholourimetric method for aspartic and alanine transaminases. Am. J. Clin. Pathol., 28, 56.CrossRefGoogle Scholar
Riddiford, L., Chen, A., Graves, B. and Curtis, A. (1981) RNA and protein synthesis during the change to pupal commitment of Manduca sexta epidermis. Insect Biochem. 11, 121–127.CrossRefGoogle Scholar
Saxena, C. and Srivastava, B. (1960) Role of certain antibiotics on the growth and reproductive performance of silkworms Bombyx mori. Proc. Natl. Acad. Sci. India Sect. B. 30, 115–119.Google Scholar
Scott, W. and Tomkins, G. (1975) The use of inhibitors in the study of hormone mechanisms in cell culture. Meth. Enz. 40, 273–292.Google Scholar
Shibko, S., Koivistoenen, P., Tratnyek, C., Newhall, A. and Freidman, L. (1967) Quantitative separation and determination of RNA and DNA in certain fungi. Anal. Biochem. 19, 514–528.CrossRefGoogle Scholar
Shigematsu, H., Kurata, K. and Takeshita, H. (1978) Nucleic acids accumulation of silk gland of Bombyx mori in relation to silk protein. Comp. Biochem. Physiol. 61, 237–242.Google Scholar
Shyamala, M., Sharada, K., Bhat, J. (1960) Chloromycetin in the nutrition of the silkworm Bombyx mori: II- Influence on digestion and utilization of protein, fat and minerals. J. Insect Physiol. 4, 229–234.CrossRefGoogle Scholar
Thayer, D. (1973) Effect of antibiotics and antimalaria on free amino acids of Aedes aegypti. J. Med. Entomol. 10, 57–62.CrossRefGoogle Scholar
Verma, S. and Kushwaha, K. (1971) Effect of four tetracycline antibiotics on the growth of silkworm Bombyx mori. Indian J. Agric. Sci. 41, 1009–1017.Google Scholar