Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-15T23:22:49.112Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)

Published online by Cambridge University Press:  01 December 2006

Paulina Quintero-Marin ,
Ana M. Caicedo ,
James Montoya-Lerma  and
Andreas Gaigl
Paulina Quintero-Marin
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia Centre Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia
Ana M. Caicedo
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia Centre Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia
James Montoya-Lerma
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia
Andreas Gaigl*
Affiliation:
Faculdad de Agronomia, Universidad Nacional de Colombia, Ciudad Universitaria, Edificio 500, Bogotá, Colombia
*
* E-mail: [email protected] or [email protected]
Get access

Abstract

The infectivity and pathogenicity of three native entomopathogenic nematodes (Heterorhabditis (HNI 0100 and sp-CIAT) and Steinernema (SNI 0198)) were evaluated in third instar larvae of Phyllophaga menetriesi (Blanchard) under laboratory conditions. Concentrations of 7000 and 13,000 infective juveniles per millilitre were applied to the coleopteran larval host (individuals) that had been placed in plastic cups with 40 g of sterile sand at 5.8% (w/w) moisture. The evaluations were done 5 and 10 days after treatment. It was observed that both the percentage of infectivity (74.5%, n = 247), measured in terms of percentage of penetration and the percentage of pathogenicity (10.5%, n = 247) of the three native nematodes were significantly different due to the treatments. Steinernema had the highest values of infectivity (>80%), but no pathogenicity. In contrast, the treatments with Heterorhabditis, despite having the lowest degree of infectivity (52.9%), had the highest mortality values (31.6%). Therefore, the Heterorhabditis strains were considered to be the most promising as biological control organisms within an integrated pest management programme due to their higher degree of pathogenicity. Nevertheless, different strains and species of native nematodes should be evaluated in all the developmental stages of the pest.

La capacité d'infection et le pouvoir pathogène des trois nématodes entomopathogènes indigènes (Heterorhabditis (HNI 0100 et sp-CIAT) et Steinernema (SNI 0198)) ont été évalués sur des larves de troisieme stada de Phyllophaga menetriesi (Blanchard) en conditions de laboratoire. Des concentrations de 7000 à 13000 juvéniles infectieux/ml ont été appliquées sur les larves du coléoptère hôte, placées dans des gobelets en plastique avec 40 g de sable stérile à 5,8% (w/w) d'humidité. Les observations ont été réalisées 5 et 10 jours après le traitement. On a constaté qu' aussi bien la capacité d'infection (74,5%; n=247), mesuré en termes de pourcentage de pénétration, que le pouvoir pathogène (10,5%; n=247) des trois nématodes indigènes, sont significativement différents. Steinernema a la capacite d'infection la plus élevée (> 80%) mais pas de pouvoir pathogène. Par contre, les Heterorhabditis qui montrent la capacite d'infection la plus faible (52,9%) ont les taux de mortalité les plus élevés (31,6%). Aussi, les souches de Heterorhabditis sont considérées comme les plus prometteuses comme agent de lutte biologique dans le cadre d'un programme de lutte intégrée contre le ravageur du fait de leur pouvoir pathogène élevé. Néanmoins, différentes souches et espèces de nématodes indigènes devront être testées sur tous les stades de développement du ravageur.

Keywords

lutte biologiqueHeterorhabditisSteinernemaVerts blancsPhyllophaga menetriesibiologique controlHeterorhabditisSteinernemaVerts blancsPhyllophaga menetriesi
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 26 , Issue 4 , December 2006 , pp. 233 - 238
Copyright
Copyright © ICIPE 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Amaya, L. M. and Bustamante, E. (1975) Control biológico de tres especies de coleópteros plagas del suelo en Colombia. Scientific Publication of the Instituto Colombiano Agropecuario (ICA) 10, 269–282.Google Scholar
Andrews, K. L. (1984) El manejo integrado de plagas invertebradas en cultivos agronómicos, hortícolas y frutales Proyecto de Manejo Integrado de Plagas de Honduras. Escuela Agrícola Panamericana, El Zamorano, Honduras.Google Scholar
Bedding, R. A. (1990) Logistics and strategies for introducing entomopathogenic nematode technology into developing countries, pp. 233–240. In Entomopathogenic Nematodes in Biological Control (Edited by Gaugler, R. and Kaya, H. K.). CRC Press, Boca Raton, FL.Google Scholar
Caicedo, A. M. and Bellotti, A. C. (1994) Evaluación del nematodo entomopatógeno Steinernema carpocapsae Weiser (Rhabditida: Steinernematidae) para el control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) en condiciones de laboratorio. Revista Colombiana de Entomologia 20, 241–246.CrossRefGoogle Scholar
Converse, V. and Grewal, P. S. (1998) Virulence of entomopathogenic nematodes to the western masked chafer Cyclocephala hirta (Coleoptera: Scarabaeidae). Journal of Economic Entomology 91, 428–432.CrossRefGoogle Scholar
Dowds, B. C. A. and Peters, A. (2002) Virulence mechanisms, pp. 78–79. In Entomopathogenic Nematology (Edited by Gaugler, R.). CAB International, Wallingford.Google Scholar
Forschler, B. and Gardner, W. (1991) Concentration-mortality response of Phyllophaga hirticula (Coleoptera: Scarabaeidae) to three entomogenous nematodes. Journal of Economic Entomology 84, 841–843.CrossRefGoogle Scholar
Garzón, M. Y., Aza, B. O., Jiménez, J. and Luque, J. E. (1996) Potencial del nematodo Steinernema sp. para el control biológico del gusano blanco de la papa. Revista Colombiana de Entomologia 22, 25–30.CrossRefGoogle Scholar
Gaugler, R., Glazer, I., Campbell, J. and Liran, N. (1994) Laboratory and field evaluation of an entomopathogenic nematode genetically selected for improved host finding. Journal of Invertebrate Pathology 63, 68–73.CrossRefGoogle Scholar
Grewal, P. S., Grewal, S. K., Malik, V. S. and Klein, M. G. (2002) Differences in susceptibility of introduced and native white grub species to entomopathogenic nematodes from various geographic localities. Biological Control 24, 230–237.CrossRefGoogle Scholar
Ishibashi, N. and Kondo, E. (1990) Behavior of infective juveniles, pp. 139–150. In Entomopathogenic Nematodes in Biological Control (Edited by Gauler, R. and Kaya, H.). CRC Press, Boca Raton, FL.Google Scholar
Kaya, H. and Stock, S. P. (1997) Techniques in insect nematology, pp. 281–324. In Manual of Techniques in Insect Pathology Biological Techniques Series (Edited by Lacey, L.). Academic Press, Wapato, WA.CrossRefGoogle Scholar
King, A. B. S. and Saunders, J. L. (1984) Las Plagas Invertebradas de Cultivos Anuales en América Central. Overseas Development Administration, London.Google Scholar
Koppenhöfer, A. M. and Fuzy, E. M. (2003) Steinernema scarabaei for the control of white grubs. Biological Control 28, 47–59.CrossRefGoogle Scholar
Londoño, M. E. (1998) La Chiza o Mojojoy, un Modelo de Investigación Entomológica, p. 82 Vol. IV No. 4. In Proceedings 4° Seminario Técnico Regional. Oct. Bucaramanga, Santander.Google Scholar
Londoño, M. E. and Pérez, M. (1994) Reconocimiento de los enemigos naturales de la chisa o mojojoy (Coleoptera: Scarabaeidae) en el oriente antioqueño. Revista Colombiana de Entomologia 20, 199–206.CrossRefGoogle Scholar
López, Y. (2001) Parasitismo natural sobre el complejo chisa (Coleoptera: Melolonthidae) en los municipios de Caldono y Buenos Aires del Departamento del Cauca. Undergraduate thesis, Facultad de Ciencias, Universidad del Valle, Cali, 30 pp.Google Scholar
Madrigal, A. (2003) Insectos Forestales en Colombia: Biología, Hábitos, Ecología y Manejo. Editorial Marín Vieco Ltda., Medellin.Google Scholar
Morón, M. A. (1986) El género Phyllophaga en México. Morfología, distribución y sistemática supraespecífica (Insecta: Coleoptera). Publicación 19. Instituto de Ecología, México, D.F.Google Scholar
Pardo, L. C. (1994) Escarabajos (Coleoptera: Melolonthidae) de importancia agrícola en Colombia, XXI Congreso Sociedad Colombiana de Entomologia 27–29 July. Medellín, 159–176.Google Scholar
Pardo, L. C. (2000) Avances en el estudio de chisas rizófagas (Coleoptera: Melolothidae) en Colombia, observaciones sobre los complejos regionales y nuevos patrones morfológicos de larvas, XXVII Congreso Sociedad Colombiana de Entomologia 26–28 July. Medellín, 285–306. SOCOLEN, Bogotá, Colombia.Google Scholar
Posada, F. (1993) Las chisas, sus enemigos naturales y recomendaciones sobre su manejo. Agricultura Tropical 30, 71–79.Google Scholar
Ramírez, L. A. (2002) Efecto de varios entomopatógenos sobre Phyllophaga menetriesi Blanchard (Coleoptera: Melolonthidae), plaga de importancia económica para el Departamento del Cauca. Undergraduate report, Universidad Nacional de Colombia, Medellín, Colombia.Google Scholar
Rosales, L. and Suárez, Z. (1998) Nematodos entomopatógenos como posibles agentes de control del gorgojo negro del plátano Cosmopolites sordidus (Germar 1824) (Coleoptera: Curculionidae). Boletín de Entomología Venezolana 13, 123–140.Google Scholar
Sáenz, A. and Luque, J. E. (1999) Cuantificación invasiva de Steinernema feltiae cepa Villapinzón en Tecia solanivora y Clavipalpus ursinus. Resúmenes XXVI Congreso Sociedad Colombiana de Entomología 28–30 July. Santafé de Bogotá. 152 pp. SOCOLEN, Bogotá, Colombia.Google Scholar
Sánchez, G. and Vásquez, N. M. (1996) Manejo de plagas en el sistema de producción de arracacha en el Departamento del Tolima. Boletín Técnico, ICA, Ibagué.Google Scholar
SAS, (1996) STAT User's Guide Version 6.4. SAS Institute, Cary, NC.Google Scholar
Sirjusingh, C., Kermarrec, A., Mauleon, H., Lavis, C. and Etienne, J. (1992) Biological control of weevils and whitegrubs on bananas and sugarcane in the Caribbean. Florida Entomologist 75, 548–562.CrossRefGoogle Scholar
Smits, P. (1992) Control of white grubs, Phyllopertha horticola and Amphimallon solstitialis in grass with heterorhabditid nematodes, pp. 229–235. In Use of Pathogens in Scarab Pest Management (Edited by Jackson, T. A. and Glare, T. R.). Intercept, Andover.Google Scholar
Tanada, Y. and Kaya, H. K. (1993) Insect Pathology. Academic Press, San Diego, CA.Google Scholar
Villani, M. G. and Wright, R. J. (1988) Entomogenous nematodes as biological control agents of European chafer and Japanese beetle (Coleoptera: Scarabaeidae) larvae infesting turfgrass. Journal of Economic Entomology 81, 484–487.CrossRefGoogle Scholar
Villani, A. G., Krueger, S. and Nyrop, J. (1992) A case study of the impact of the environment on insect/pathogen interactions: Scarabs in turfgrass, pp. 111–126. In Use of Pathogens in Scarab Pest Management (Edited by Jackson, T. A. and Glare, T. R.). Intercept, Andover.Google Scholar
Wang, Y. and Gaugler, R. (1998) Host and penetration site location by entomopathogenic nematodes against Japanese beetle larvae. Journal of Invertebrate Pathology 72, 313–318.CrossRefGoogle ScholarPubMed
Wright, R. J., Villani, M. G. and Agudelo-Silva, F. (1988) Steinernematid and heterorhabditid nematodes for control of larval European chafers and Japanese beetles (Coleoptera: Scarabaeidae) in potted yew. Journal of Economic Entomology 81, 152–157.CrossRefGoogle Scholar