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Identifying triatomine symbiont Rhodococcus rhodnii as intestinal bacteria from Rhodnius ecuadoriensis (Hemiptera: Reduviidae) laboratory insects

Published online by Cambridge University Press:  07 March 2011

Janeth Rodríguez ,
Paula Pavía ,
Marleny Montilla  and
Concepción J. Puerta
Janeth Rodríguez
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
Paula Pavía
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
Marleny Montilla
Affiliation:
Grupo de Parasitología, Instituto Nacional de Salud, Avenida Calle 26, #51-20, Bogotá, Colombia
Concepción J. Puerta*
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
*
*E-mail: [email protected]
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Abstract

The Nocardiaceae family members are symbiont bacteria from triatomine insects. The aim of this study was to detect the presence of Nocardiaceae family bacteria in Rhodnius ecuadoriensis intestinal bacterial flora. Bacterial colonies were isolated from the intestinal content from all insects; they had pink or coral pink pigmentation corresponding to Gram-positive coccobacilli. These isolates' biochemical identification by catalase and urease tests and the assimilation of different carbon sources led to Rhodococcus rhodnii being identified. This result was confirmed by 16S ribosomal ribonucleic acid gene sequencing. Additional studies are required to establish the symbiont role of this bacterium in R. ecuadoriensis as it has in Rhodnius prolixus.

Keywords

Rhodococcus rhodniiRhodnius ecuadoriensisChagas disease
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 34 - 37
Copyright
Copyright © ICIPE 2011

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