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Disinfection of a Probe Used in Ultrasound-Guided Prostate Biopsy

Published online by Cambridge University Press:  02 January 2015

William A. Rutala ,
Maria F. Gergen  and
David J. Weber
William A. Rutala*
Affiliation:
Department of Hospital Epidemiology, University of North Carolina Health Care System, Chapel Hill Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill
Maria F. Gergen
Affiliation:
Department of Hospital Epidemiology, University of North Carolina Health Care System, Chapel Hill
David J. Weber
Affiliation:
Department of Hospital Epidemiology, University of North Carolina Health Care System, Chapel Hill Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill
*
Division of Infectious Diseases, 130 Mason Farm Road, Bioinformatics, UNC at Chapel Hill, Chapel Hill, NC 27599-7030 ([email protected])
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Abstract

Background.

Transrectal ultrasound (TRUS)-guided prostate biopsies are among the most common outpatient diagnostic procedures in urology clinics and carry the risk of introducing pathogens that may lead to infection.

Objective.

To investigate the effectiveness of procedures for disinfecting a probe used in ultrasound-guided prostate biopsy.

Method.

The effectiveness of disinfection was determined by inoculating 107 colony forming units (cfu) of Pseudomonas aeruginosa at the following 3 sites on the probe: the interior lumen of the biopsy needle guide, the outside surface of the biopsy needle guide, and the interior lumen of the ultrasound probe where the needle guide passes through the transducer. Each site was investigated separately. After inoculation, the probe was immersed in 2% glutaraldehyde for 20 minutes and then assessed for the level of microbial contamination.

Results.

The results demonstrated that disinfection (ie, a reduction in bacterial load of greater than 7 log10 cfu) could be achieved if the needle guide was removed from the probe. However, if the needle guide was left in the probe channel during immersion in 2% glutaraldehyde, disinfection was not achieved (ie, the reduction was approximately 1 log10 cfu).

Conclusions.

Recommendations for probe disinfection are provided and include disassembling the device and immersing the probe and the needle guide separately in a high-level disinfectant.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 28 , Issue 8 , August 2007 , pp. 916 - 919
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2007

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