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Laboratory-based study of novel antimicrobial cold spray coatings to combat surface microbial contamination

Published online by Cambridge University Press:  19 August 2020

M.D.I. Lucas ,
I. Botef ,
R. G. Reid Open the ORCID record for R. G. Reid [Opens in a new window]  and
S. F. van Vuuren Open the ORCID record for S. F. van Vuuren [Opens in a new window]
M.D.I. Lucas
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
I. Botef
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
R. G. Reid
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
S. F. van Vuuren*
Affiliation:
Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg, South Africa
*
Author for correspondence: Sandy van Vuuren, Email: [email protected]
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Abstract

Objective:

To investigate the touch-contact antimicrobial efficacy of novel cold spray surface coatings composed of copper and silver metals, regard to their rate of microbial elimination.

Design:

Antimicrobial time-kill assay.

Setting:

Laboratory-based study.

Methods:

An adapted time-kill assay was conducted to characterize the antimicrobial efficacy of the developed coatings. A simulated touch-contact pathogenic exposure to Gram-positive Staphylococcus aureus (ATCC 25923), Gram-negative Pseudomonas aeruginosa (ATCC 27853), and the yeast Candida albicans (ATCC 10231), as well as corresponding resistant strains of gentamicin-methicillin–resistant S. aureus (ATCC 33592), azlocillin-carbenicillin–resistant P. aeruginosa (DSM 46316), and a fluconazole-resistant C. albicans strain was undertaken. Linear regression modeling was used to deduce microbial reduction rates.

Results:

A >7 log reduction in microbial colony forming units was achieved within minutes on surfaces with cold spray coatings compared to a single log bacterial reduction on copper metal sheets within a 3 hour contact period. Copper-coated 3-dimensional (3D) printed acrylonitrile butadiene styrene (ABS) achieved complete microbial elimination against all tested pathogens within a 15 minute exposure period. Similarly, a copper-on-copper coating achieved microbial elimination within 10 minutes and within 5 minutes with the addition of silver powder as a 5 wt% coating constituent.

Conclusions:

In response to the global need for alternative solutions for infection control and prevention, these effective antimicrobial surface coatings were proposed. A longitudinal study is the next step toward technology integration.

Type
Original Article
Information
Infection Control & Hospital Epidemiology , Volume 41 , Issue 12 , December 2020 , pp. 1378 - 1383
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Copyright
© 2020 by The Society for Healthcare Epidemiology of America. All rights reserved.

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Footnotes

a

Deceased.

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