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Studies on the inhibition and mutation of Aspergillus nidulans by acridines

Published online by Cambridge University Press:  14 April 2009

C. Ball  and
J. A. Roper
C. Ball
Affiliation:
Department of Genetics, University of Sheffield
J. A. Roper
Affiliation:
Department of Genetics, University of Sheffield
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A number of acridines have been tested for ability to inhibit conidia of strains of Aspergillus nidulans. The effectiveness of any one acridine in growth inhibition and killing involves interaction of genotype and conditions of treatment such as temperature, pH, treatment medium and light intensity. Mutant alleles which confer growth resistance to acriflavine are selective in their actions towards other acridines, may differ in their dominance relationships with different acridines and are even selective with regard to the conditions under which they confer acriflavine resistance. Certain pairs of acridines, used simultaneously, show additive effects, potentiation, or annulment by one of inhibition caused by the other.

Some of these findings have been applied in a study of factors affecting acridine-induced mutation in Aspergillus conidia. Under conditions which permit metabolism, acriflavine induces a high frequency of unstable morphological variants. One such variant has been shown to be a disomic. Using a system of reversion from auxotrophy to prototrophy, acriflavine-induced mutation has been obtained both with high light intensities and in the absence of light. In the latter case recombination as a feature of the mutation process is excluded.

Type
Research Article
Information
Genetics Research , Volume 7 , Issue 2 , April 1966 , pp. 207 - 221
Copyright
Copyright © Cambridge University Press 1966

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