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Six new loci controlling resistance to p-fluorophenylalanine in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

Sheela Srivastava  and
Umakant Sinha
Sheela Srivastava
Affiliation:
Department of Botany, University of Delhi, Delhi-110007, India
Umakant Sinha
Affiliation:
Department of Botany, University of Delhi, Delhi-110007, India
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Twelve FPA-resistant mutants were selected on medium containing p-fluorophenylalanine and ethionine. Dominance tests in heterozygous diploids showed that 8 out of 12 are dominant and 4 recessive to their wild-type alleles. One mutant, fpa60, showed a partial requirement for tyrosine and was found to be allelic to an fpaA mutant described previously. A tyrosine non-requirer, fpa65, was also assigned to this locus. The other 10 mutants did not show any growth requirement and were simultaneously resistant to ethionine and 3-amino-L-tyrosine. Of the 8 dominant mutants, 3 were allelic to the permease-mutants at the locus fpaD. Dominant mutants showed higher degrees of resistance than recessive ones. Six new loci, identified after preliminary genetic analysis, were located on 3 linkage groups: 3 on linkage group VI, and one each on linkage groups I, V, and VIII. The recombinant fpaD11; fpaK69 was found to be sensitive to FPA.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 1 , February 1975 , pp. 29 - 38
Copyright
Copyright © Cambridge University Press 1975

References

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