Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T11:37:25.640Z Has data issue: false hasContentIssue false
  • English
  • Français

A maternal genetic effect on the composition of mouse aggregation chimaeras

Published online by Cambridge University Press:  14 April 2009

John D. West ,
Jean H. Flockhart  and
Adrien Kissenpfennig
John D. West
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK
Jean H. Flockhart
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK
Adrien Kissenpfennig
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two series of 12½ day mouse chimaeric conceptuses were produced by aggregating (C57BL × CBA)F2 strain preimplantation embryos with embryos that differed at the Gpi-1s locus that encodes glucose phosphate isomerase, GPI-1. The composition of individual issues was evaluated by quantitative electrophoresis to estimate the % GPI-1A in the chimaeric tissue containing GPI-1A and GPI-1B. In one series of chimaeras, the GPI-1A cells were derived from a backcross between inbred BALB/c strain females and (BC × BALB/c)F1 males, where BC is the partly congenic strain C57BL/Ola.AKR-Gpi-lsa,c/Ws. In the other series of chimaeras, the GPI-1A cells were derived from the reciprocal backcross between (BC × BALB/c)F1 females and inbred BALB/c strain males. The [(BC × BALB/c)F1 female × BALB/c male] ↔ (C57BL × CBA)F2 series of chimaeras was reasonably balanced so that GPI-1 A and GPI-1B cells were fairly equally represented in the foetuses, placentas and extraembryonic membranes (tissue means: 37–51 % GPI-1A). This series did not differ significantly in composition from an earlier series of (BC × BALB/c)F2 ↔ (C57BL × CBA)F2 chimaeras. However, the [BALB/c female × (BC × BALB/c)F1 male] ↔ (C57BL × CBA)F2 series of chimaeras was unbalanced, with mean tissue compositions (28–33% GPI-1A) that were intermediate between the above two balanced series and the unbalanced (BALB/c × BALB/c) ↔ (C57BL × CBA)F2 series (tissue means: 14–22% GPI-1 A), that was studied previously. Thus, both (BALB/c×BALB/c) and [BALB/c×(BC x BALB/c)F1 embryos contributed less to the tissues of chimaeric conceptuses than either (BC × BALB/c)F2or [(BC × BALB/c)F1 × BALB/c] embryos. This implies that embryos from BALB/c mothers contributed less to the tissues of chimaeric conceptuses than embryos from (BC × BALB/c)F1 mothers. We, therefore, conclude that a maternal genetic effect is responsible for some of the differences in composition among the four groups of chimaeras. This maternal effect must act before the 8-cell stage but it is not yet known whether it is mediated via cytoplasmic inheritance, genomic imprinting or by the reproductive tract. Evidence that a maternal effect retards preimplantation development of embryos from BALB/c females is reviewed and the possibility that this might cause them to contribute poorly to chimaeric conceptuses when aggregated with more precociously developing embryos is discussed.

Type
Research Article
Information
Genetics Research , Volume 65 , Issue 1 , February 1995 , pp. 29 - 40
Copyright
Copyright © Cambridge University Press 1995

References

Copp, A. J., (1978). Interaction between inner cell mass and trophectoderm of the mouse blastocyst. I. A study of cellular proliferation. Journal of Embryology and Experimental Morphology 48, 109–125.Google Scholar
Copp, A. J., (1979). Interaction between inner cell mass and trophectoderm of the mouse blastocyst. II. The fate of the polar trophectoderm. Journal of Embryology and Experimental Morphology 51, 109–120.Google Scholar
Cruz, Y. P., & Pederson, R. A., (1985). Cell fate in the polar trophectoderm of mouse blastocysts as studied by microinjection of cell lineage tracers. Developmental Biology 112, 73–83.Google Scholar
Dyce, J., George, M., Goodhall, H., & Fleming, T., (1987). Do trophectoderm and inner cell mass cells in the mouse blastocyst maintain discrete lineages? Development 100, 685–698.Google Scholar
Edirisinghe, W. R., Wales, R. G., & Pike, I. L., (1984). Studies of the distribution of glycogen between inner cell mass and trophoblast cells of mouse embryos. Journal of Reproduction and Fertility 71, 533–538.Google Scholar
Falconer, D. S., & Avery, P. J., (1978). Variability of chimaeras and mosaics. Journal of Embryology and Experimental Morphology 43, 195–219.Google ScholarPubMed
Gardner, R. L., & Papaioannou, V. E., (1975). Differentiation in the trophectoderm and inner cell mass. In The Early Development of Mammals. The Second Symposium of the British Society for Developmental Biology (ed. Balls, M. and Wild, A. E.), pp. 107–132. Cambridge: Cambridge University Press.Google Scholar
Handyside, A. H., (1978). Time of commitment of inside cells isolated from preimplantation mouse embryos. Journal of Embryology and Experimental Morphology 45, 37–53.Google Scholar
Handyside, A. H., & Hunter, S., (1986). Cell division and death in the mouse blastocyst before implantation. Roux's Archives of Developmental Biology 195, 519–526.Google Scholar
James, R., Flockhart, J. H., Keighren, M., & West, J. D., (1993). Quantitative analysis of mid-gestation mouse aggregation chimaeras: non-random composition of the placenta. Roux's Archives of Developmental Biology 202, 296–305.Google Scholar
Lo, C., (1983). Transformation by iontophoretic microinjection of DNA: multiple integrations without tandem insertions. Molecular and Cellular Biology 3, 1803–1814.Google Scholar
Lo, C., (1986). Localization of low abundance DNA sequences in tissue sections by in situ hybridization. Journal of Cell Science 8, 143–162.Google Scholar
McLaren, A., (1981) Analysis of maternal effects on development in mammals. Journal of Reproduction and Fertility 62, 591–596.Google Scholar
McLaren, A., & Bowman, P., (1973). Genetic effects on the timing of early development in the mouse. Journal of Embryology and Experimental Morphology 30, 491–498.Google ScholarPubMed
McLaren, A., & Buehr, M., (1990). Development of mouse germ cells in cultures of fetal gonads. Cell Differentiation and Development 31, 185–195.CrossRefGoogle ScholarPubMed
Mullen, R. J., & Whitten, W. K., (1971). Relationship of genotype and degree of coat colour to sex ratios and gametogenesis in chimaeric mice. Journal of Experimental Zoology 178, 165–176.CrossRefGoogle Scholar
Nicol, A., & McLaren, A., (1974). An effect of the female genotype on sperm transport in mice. Journal of Reproduction and Fertility 39, 421–424.Google Scholar
Palmer, S. J., & Burgoyne, P. S., (1991). The Mus musculus domesticus Tdy allele acts later than the Mus musculus musculus Tdy allele: a basis for XY sex reversal in C57BL/6-YPOS mice. Development 113, 709–714.CrossRefGoogle Scholar
Shire, J. G. M., & Whitten, W. K. (1980 a). Genetic variation in the timing of first cleavage: effect of paternal genotype. Biology of Reproduction 23, 363–368.Google Scholar
Shire, J. G. M., & Whitten, W. K. (1980 b). Genetic variation in the timing of first cleavage in mice: effect of maternal genotype. Biology of Reproduction 23, 369–376.Google Scholar
Spindle, A., (1982). Cell allocation in preimplantation mouse chimeras. Journal of Experimental Zoology 219, 361–367.CrossRefGoogle ScholarPubMed
West, J. D., Bücher, T., Linke, I. M., & Dünnwald, M., (1984). Investigation of variability among mouse aggregation chimaeras and X chromosome inactivation mosaics. Journal of Embryology and Experimental Morphology 84, 309–329.Google ScholarPubMed
West, J. D., & Flockhart, J. H., (1994). Genotypically unbalanced diploid ↔ diploid foetal mouse chimaeras: possible relevance to human confined mosaicism. Genetical Research 63, 87–99.CrossRefGoogle ScholarPubMed
West, J. D., Flockhart, J. H., & Keighren, M., (1995). Biochemical evidence for cell fusion in placentas of mouse aggregation chimeras. Developmental Biology. In press.CrossRefGoogle Scholar
Whitten, W. K., & Dagg, C. P., (1961). Influence of spermatozoa on the cleavage rate of mouse eggs. Journal of Experimental Zoology 148, 173–183.Google Scholar
Winkel, G. K., & Pedersen, R. A., (1988). Fate of the inner cell mass in mouse embryos studied by microinjection of lineage tracer. Developmental Biology 127, 143–156.CrossRefGoogle Scholar