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Location of genes for the utilization of acetamide, histidine and proline on the chromosome of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

Martin Day ,
J. Rowland Potts  and
Patricia H. Clarke
Martin Day
Affiliation:
Department of Biochemistry, University College London, Gower St, London WC1E 6BT
J. Rowland Potts
Affiliation:
Department of Biochemistry, University College London, Gower St, London WC1E 6BT
Patricia H. Clarke
Affiliation:
Department of Biochemistry, University College London, Gower St, London WC1E 6BT
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Summary

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Interrupted mating methods were used to map the positions on the chromosome of genes for the utilization of acetamide, histidine and proline. An entry time of 22 min was found for a put marker determining the utilization of proline. Two groups of hut mutants were studied. Mutation hut-109 was located at 18 min and mutation hutU108, which results in a defect in urocanase activity, mapped at 28 min. hutU was shown by transduction with bacteriophage F116 to be linked to ser-3 but not to met-28 and ilv-202, giving a map order of hutU-ser-3-ilv-202-met-28.

The amidase genes were assigned an entry time of 65–75 min. A method is described for examining late entry markers by interrupted mating.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 1 , February 1975 , pp. 71 - 78
Copyright
Copyright © Cambridge University Press 1975

References

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