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Efficient curing of an Escherichia coli F-prime plasmid by phenothiazines

Published online by Cambridge University Press:  14 April 2009

Y. Mándi ,
J. Molnár ,
I. B. Holland  and
I. Béládi
Y. Mándi
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
J. Molnár
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
I. B. Holland
Affiliation:
Department of Genetics, University of Leicester, Leicester, England
I. Béládi
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary

Summary

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Chlorpromazine and several other phenothiazines at sub-bacteriocidal concentrations were found to cure an Escherichia coli F′ lac+ strain of its plasmid efficiently. Curing was most efficient at high pH and in complex medium when 70% or more of the bacteria were plasmid-free after 24 h growth of cultures in the presence of the drug.

Type
Short paper
Information
Genetics Research , Volume 26 , Issue 1 , August 1975 , pp. 109 - 111
Copyright
Copyright © Cambridge University Press 1975

References

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