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Analysis of the resistance mediated by several R-factors to Tetracycline and Minocycline

Published online by Cambridge University Press:  14 April 2009

J. M. Robertson  and
E. C. R. Reeve
J. M. Robertson
Affiliation:
Institute of Animal Genetics, West Mains Mood, Edinburgh, EH9 3JN, Scotland
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Mood, Edinburgh, EH9 3JN, Scotland
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The resistance levels conferred by the T-determinants in four R-factors to Tetracycline and Minocycline in cells of Escherichia coli K 12, before and after induction of maximum resistance by treatment with sub-inhibitory concentrations of the drugs, are measured by simple growth-and-challenge tests. The effect of a plasmid TK which confers tetracycline resistance on its host Klebsiella aerogenes is tested in the same way. The five T-determinants fall into a high-level and a low-level group for resistance, the former giving 3- to 4-fold higher resistance in both induced and uninduced cells than the latter. The T-determinants all confer much lower resistance to Minocycline (a tetracycline molecule modified at the C-6 and C-7 positions) than to Tetracycline. The main cause of this difference is that cells carrying a T-determinant exclude Minocycline much less efficiently than Tetracycline, but in addition Minocycline is less effective than Tetracycline in inducing increased resistance. These results are discussed in the light of a model put forward to explain the inducible nature of R-factor resistance to the tetracyclines.

Type
Research Article
Information
Genetics Research , Volume 20 , Issue 2 , October 1972 , pp. 239 - 252
Copyright
Copyright © Cambridge University Press 1972

References

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