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An easy way of obtaining Aspergillus nidulans haploids in the parasexual cycle using N-glycosyl polifungin

Published online by Cambridge University Press:  14 April 2009

Jerzy Bal ,
Ewa Bartnik ,
Bożenna Goryluk  and
Norman J. Pieniążek
Jerzy Bal
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Ewa Bartnik
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Bożenna Goryluk
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Norman J. Pieniążek
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
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Summary

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A considerable improvement in the selection of haploids in Aspergillus nidulans is described. Haploidization is used to assign genes to linkage groups (chromosomes) in fungi. The differential effect of a polyene antibiotic, N-glycosyl-polifungin, in respect to haploids and diploids, was utilized. In minimal medium only diploids (constructed from a ‘Master Strain’ and the investigated strain), which are fully heterozygous and thus have no growth requirements, germinate and are killed by the antibiotic. However, auxotrophic haploids, either induced or spontaneously formed, survive. These can then be used for linkage studies.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 3 , June 1975 , pp. 249 - 252
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Bal, J., Balbin, E. & Pieniążek, N. J. (1974). Method for selecting auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin. Journal of General Microbiology 84, 111–116.CrossRefGoogle Scholar
Cove, D. J. (1966). The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta 113, 51–56.CrossRefGoogle ScholarPubMed
Ditchburn, P. & MacDondald, K. D. (1971). The differential effect of nystatin on growth of auxotrophic and prototrophic strains of Aspergillus nidulans. Journal of General Microbiology 67, 299–306.CrossRefGoogle ScholarPubMed
Falkowski, L., Cybulska, B., Zabłocka, E. and Borowski, E. (1973). N-glycosyl derivatives of polyene macrolide antifungal antibiotics in vitro studies. Proceedings of the VIIIth International Congress of Chemoterapiae, Athens. Athens.Google Scholar
Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13, 67–80.CrossRefGoogle Scholar
Hamilton-Miller, J. M. T. (1973). Chemistry and biology of the polyene macrolide antibiotics. Bacteriological Reviews 37, 166–196.CrossRefGoogle ScholarPubMed
Käker, E. (1961). The processes of spontaneous recombination in vegetative nuclei of A. nidulans. Genetics 46, 1581–1609.Google Scholar
Lhoas, P. (1961). Mitotic haploidisation by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature 190, 744.CrossRefGoogle Scholar
Lhoas, P. (1968). Growth rate and haploidization of Aspergillus niger on medium containing p-fluorophenylalanine. Genetical Research 12, 305–315.CrossRefGoogle ScholarPubMed
McCully, K. S. & Forbes, E. (1965). The use of p-fluorophenylalanine with ‘Master Strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6, 352–359.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141–238.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Gloor, E. Tarr & Forbes, E. (1954). Analysis of mitotic recombination in Aspergillus nidulans. Journal of Genetics 52, 226–237.CrossRefGoogle Scholar
Roberts, C. F. (1963). The genetic analysis of carbohydrate utilization in Aspergillus nidulans. Journal of General Microbiology 31, 45–58.CrossRefGoogle ScholarPubMed