Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-12-02T19:40:33.904Z Has data issue: false hasContentIssue false
  • English
  • Français

Methods of Identifying Genetic Diversity in Gliricidia Species for Biomass Production

Published online by Cambridge University Press:  03 October 2008

J. R. Chamberlain  and
N. W. Galwey
J. R. Chamberlain
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
N. W. Galwey
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
Get access Rights & Permissions [Opens in a new window]

Summary

Gliricidia sepium is a leguminous tree native to Mexico and Central America, and is utilized for fuelwood, animal fodder and green manure in rural communities. It is widely distributed throughout the tropics, but many populations of the tree have arisen from haphazard introductions of unknown quality. Methods for determining the genetic structure and origin of populations are therefore required as a basis for genetic improvement. In order to test the effectiveness of various measures of genetic diversity, provenances of G. sepium from Panama, Guatemala, Mexico and Honduras were studied by electrophoresis of seed storage proteins and the enzymes leucine aminopeptidase (LAP) and acid phosphatase (ACP), and by measurement of plant morphological characteristics. Study of seed storage proteins and morphological characters was very informative, and there was some genetic variation for ACP, but LAP showed no clear variation. In the cases where genetic variation was present, the different methods of study showed a consistent pattern of variation: genetic diversity was greatest in the population from Honduras, and least in that from Panama. This was contrary to expectations on the basis of the locations of origin of the populations and the range in which G. sepium is believed to be native. Electrophoresis of the storage proteins of Gliricidia maculata confirmed the status of this species as a distinct taxon.

Type
Research Article
Information
Experimental Agriculture , Volume 29 , Issue 1 , January 1993 , pp. 87 - 96
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brewbaker, J. L. (1987). Leucaena: a multipurpose tree genus for tropical agroforestry. In Agooforestry: a Decade of Development, 289–318 (Eds Steppler, H. A. and Nair, P. K.). Nairobi, Kenya: ICRAF.Google Scholar
Falvey, J. L. (1982). Gliricidia maculata-a review. International Tree Crops Journal 2:1–14.CrossRefGoogle Scholar
Hughes, C. E. (1986). Progress in collection and evaluation of NFT germplasm from Central America. Nitrogen-Fixing Tree Research Reports 4:49–51.Google Scholar
Hughes, C. E. & Styles, B. T. (1984). Exploration and seed collection of dry zone multipurpose trees in Central America. International Tree Crops Journal 3:1–31.CrossRefGoogle Scholar
Smith, D. B. & Payne, P. I. (1984). A procedure for the routine determination of electrophoretic band patterns of barley and malt endosperm proteins. Journal of the National Institute of Agricultural Botany 16:487–498.Google Scholar
Shaw, C. R. & Prasad, R. (1970). Starch gel electrophoresis of enzymes-a compilation of recipes. Biochemical Genetics 4:297–320.CrossRefGoogle ScholarPubMed