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The dexamethasone suppression test (DST) and effects of in vitro and in vivo glucocorticoids on mitogen-induced lymphocyte proliferation in major depression

Published online by Cambridge University Press:  16 April 2020

M Pötzsch ,
C Schiütt  and
K Ernst
M Pötzsch
Affiliation:
Department of Medical Immunology, Ernst-Moritz-Arndt University, Fleischmannstr, 0-2200Greifswald
C Schiütt
Affiliation:
Department of Medical Immunology, Ernst-Moritz-Arndt University, Fleischmannstr, 0-2200Greifswald
K Ernst
Affiliation:
Department of Psychiatry, University of Rostock, Gehlsheimer Str, 20, 0-2540Rostock 40, Germany
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Summary

To prove the hypothesis of a common defect in the central nervous system and immune system in patients with major depression, we examined the inhibition of phytohemagglutinin (PHA)-induced lymphocyte proliferation by in vitro and in vivo administration of glucocorticoids in 20 patients with major depression. The patients were divided into two groups according to the results of the dexamethasone suppression test (DST). Six patients showed cortisol non-suppression. They had significantly higher baseline plasma cortisol levels in comparison with cortisol suppressors. The lymphocytes from cortisol non-suppressors were significantly stronger inhibited by in vitro administration of glucocorticoids compared to the lymphocytes from cortisol suppressors. However, there were no significant differences of lymphocyte inhibition by in vivo administration of glucocorticoids between both patient groups. From our results we conclude that cortisol non-suppressors reveal impaired down-regulation of glucocorticoid receptors of lymphocytes. Furthermore, the effect of glucocorticoids on lymphocyte reactivity may reflect the effects on brain structures in patients with major depression.

Keywords

DSTmitogen-induced lymphocyte proliferation testmajor depressionglucocorticoids
Type
Original article
Information
European Psychiatry , Volume 7 , Issue 1 , 1992 , pp. 21 - 25
Copyright
Copyright © Elsevier, Paris 1992

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References

Besedovsky, HODel Rey, ASorkin, ELotz, WSchwulera, U (1985) Lymphoid cells produce an immunoregulatory glucocorticoid increasing factor (GIF) acting through the pituitary gland Clin Exp Immunol 59, 622–688Google ScholarPubMed
Besedowsky, HODel Rey, ASorkin, E (1986) Integration of activated immune cell products in immuneendocrine feed-back circuitsIn: Leukocytes and Host Defence Liss, New York, pp 197–404Google Scholar
Carroll, BJ (1982a) Clinical applications of the dexamethasone suppression test for endogenous depression Pharmacopsychiatry 15, 19–24CrossRefGoogle Scholar
Carroll, BJ (1982b) The dexamethasone suppression test for melancholia, Br J Psychiatry 140, 292–304CrossRefGoogle Scholar
Crupps, TRFauci, AS (1982) Corticosteroid-mediated immunoregulation in man Immunol Rev 65, 133–155CrossRefGoogle Scholar
Darko, FGillin, JCRish, SCBulloch, KGolshan, STasevska, ZHamburger, RN (1989) Mitogen-stimulated lymphocyte proliferation and pituitary hormones in major depression Biol Psychiatry 26, 145–155CrossRefGoogle ScholarPubMed
Gillis, SCrabtree, GRSmith, KA (1979) Glucocorticoidinduced inhibition of T cell growth factor production. I. The effect on mitogen-induced lymphocite proliferation J Immunol 123, 1624–1631Google Scholar
Gillis, SCrabtree, GRSmith, KA (1979) Glucocorticoidinduced inhibition of T cell growth factor production. II. The effect on the in vitro generation of cytolytic T cells J Immunol 123, 1632–1638Google Scholar
Härfstrand, AFuxe, KCintra, AAgnali, LFZini, IWirk strÖm, ACOkret, SYu, ZYGoldstein, MSteinbush, HVerhofstad, AGustafson, HA (1986) Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain Proc Natl Acad Sci USA 83,97779–9783CrossRefGoogle ScholarPubMed
Irwin, MSmith, TLGillin, JC (1987) Low natural killer cytotoxicity in major depression Life Sci 41, 2127–2133CrossRefGoogle ScholarPubMed
Izawa, MSatoh, Yichii, S (1983) Recycling of steroid receptorsIn: Hormone Receptors and Receptor Disease (Imura, HKuzaya, H eds) Excerpta Medica, Amsterdam, 127-135Google Scholar
Jones, MTHillhouse, EW (1977) Neurotransmitter regulation of corticotropin-releasing factor in vitroACTH Related Peptides: Structure, Regulation and Action (Krieger, DTGanong, WF eds) Acad Sci New York, 536–550Google Scholar
Kronfol, ZHouse, JDSilva, JGreden, JCaroll, BJ (1986) Depression, urinary free Cortisol excretion and lymphocyte function Br J Psychiatry 148, 70–73CrossRefGoogle ScholarPubMed
Lowy, MTReder, ATAntel, JPMeltzer, HY (1984) Gluco-corticoid resistance in depression: The dexamethasone suppression test and lymphocyte sensitivity to dexamethasone Am J Psychiatry 141, 1365–1370Google Scholar
De Nicola, AFTornello, SCoirini, HHeller, COrti, EWhite, AMarusik, ET (1982) Regulation of receptors lor gluco- and mineralocorticoidsIn: Physiopathology of Hypophysial Disturbances and Diseases of Reproduction Liss, New York, 61–85Google Scholar
Parillo, JEFauci, AS (1979) Mechanisms of glucocorticoid action on immune processess Ann Rev Pharmacol Toxicol 19, 179–201CrossRefGoogle Scholar
PÖtzseh, MSchütt, CErnst, K (1991) Mitogene Lymphozytenstimulierbarkeit und Dexamethasonsuppressions test bei endogener Depression. Psychiutria Danuhina (in press)Google Scholar
Rubin, RTPoland, RELesser, IMWinston, RABlodgett, ALN (1987) Neuroendocrine aspects of primary endogenous depression. I. Cortisol secretory dynamics in patients and matched controls Arch Gen Psychiatry 44, 328–336CrossRefGoogle ScholarPubMed
Schlechte, JAGinsberg, BHSherman, BM (1982) Regulation of the glucocorticoid receptor in human lymphocytes J Steroid Biochem 16, 69–74CrossRefGoogle ScholarPubMed
Schleifer, SJKeller, SEMeyerson, ATRaskind, MJDavis, KLStein, M (1984) Lymphocyte function in major depressive disorder Arch Gen Psychiatry 41, 484–486CrossRefGoogle ScholarPubMed
Schütt, C (1982) Zur Aussagekraft des Lymphozytentransformnationstestcs in der klinischen Diagnostic. Diss B, Rostock, Univ, Med Fak Schiitt C (1990) Das neuroimmunoendokrine SystemIn: Grundrih der Immunhiologic (Ambrosius, HRudolph, W eds) Fischer, Jena pp 384–395Google Scholar
Schütt, CHarms, LTemplin, BUSchulzc, HA (1978) Die H-Thymidin-Plattchenmodifikation des Lymphozytentransformationstests. I. Kultivierungsbedingungen fiir Menschen- sowie Mäuselymphozyten und Reproduzierbarkeit der Papier-Plättehen-Teehnik Acta Biol Med Ger 37, 1091–1097Google Scholar
Schütt, CSÜss, JKramer, HGÖring, HD (1979) Tagungsberichte. Bericht Über das II. LTT-Arbcitstreffen Allerg Immuno 25, 84–86Google Scholar
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