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Effects of chronic occupational exposure to anaesthetic gases on the rate of neutrophil apoptosis among anaesthetists

Published online by Cambridge University Press:  16 August 2006

R. Tyther ,
M. Halligan ,
J. Wang ,
H. P. Redmond  and
G. Shorten
R. Tyther
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, University College Cork, Cork, Ireland
M. Halligan
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, University College Cork, Cork, Ireland
J. Wang
Affiliation:
Cork University Hospital, Department of Surgery, and University College Cork, Cork, Ireland
H. P. Redmond
Affiliation:
Cork University Hospital, Department of Surgery, and University College Cork, Cork, Ireland
G. Shorten
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, University College Cork, Cork, Ireland
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Abstract

Background and objective: Volatile anaesthetic agents are known to influence neutrophil function. The aim was to determine the effect of chronic occupational exposure to volatile anaesthetic agents on the rate of neutrophil apoptosis among anaesthetists. To test this hypothesis, we compared the rate of neutrophil apoptosis in anaesthetists who had been chronically exposed to volatile anaesthetic agents with that in unexposed volunteers.

Methods: Venous blood (20 mL) was withdrawn from 24 ASA I-II volunteers, from which neutrophils were isolated, and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide.

Results: At 1 h (but not at 12 and 24 h) in culture, the rate of neutrophil apoptosis was significantly less in the anaesthetists – 13.8 (12.9%) versus 34.4 (12.1%) (P = 0.001).

Conclusions: Chronic occupational exposure to volatile anaesthetic agents may inhibit neutrophil apoptosis. This may have implications for anaesthetists and similarly exposed healthcare workers in terms of the adequacy of their inflammatory response.

Keywords

anaesthesiacell deathapoptosispathological processesinflammationimmune systemleukocytesgranulocytesneutrophilsenvironmental pollutionenvironmental exposureoccupational exposure
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 19 , Issue 8 , August 2002 , pp. 604 - 608
Copyright
2002 European Society of Anaesthesiology

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