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Effect of sevoflurane on human neutrophil apoptosis

Published online by Cambridge University Press:  02 June 2005

R. Tyther ,
J. O'Brien ,
J. Wang ,
H. P. Redmond  and
G. Shorten
R. Tyther
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, Cork, Ireland
J. O'Brien
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, Cork, Ireland
J. Wang
Affiliation:
Cork University Hospital, Department of Surgery, Cork, and University College Cork, Cork, Ireland
H. P. Redmond
Affiliation:
Cork University Hospital, Department of Surgery, Cork, and University College Cork, Cork, Ireland
G. Shorten
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, Cork, Ireland
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Abstract

Summary

Background and objective: Both chronic occupational exposure to volatile anaesthetic agents and acute in vitro exposure of neutrophils to isoflurane have been shown to inhibit the rate of apoptosis of human neutrophils. It is possible that inhibition of neutrophil apoptosis arises through delaying mitochondrial membrane potential collapse. We assessed mitochondrial depolarization and apoptosis in unexposed neutrophils and neutrophils exposed to sevoflurane in vivo.

Methods: A total of 20 mL venous blood was withdrawn pre- and postinduction of anaesthesia, the neutrophils isolated and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide. Mitochondrial depolarization was measured using the dual emission styryl dye JC-1.

Results: Apoptosis was significantly inhibited in neutrophils exposed to sevoflurane in vivo at 24 (exposed: 38 (12)% versus control: 28 (11)%, P = 0.001), but not at 1 or 12 h, in culture. Mitochondrial depolarization was not delayed in neutrophils exposed to sevoflurane.

Conclusions: The most important findings are that sevoflurane inhibits neutrophil apoptosis in vivo and that inhibition is not mediated primarily by an effect on mitochondrial depolarization.

Keywords

ANAESTHESIA GENERAL, anaesthesia, inhalation, sevofluraneBLOOD CELLS, leukocytes, granulocytes, neutrophilsCELL DEATH, apoptosisPATHOLOGICAL PROCESSES, inflammation
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 20 , Issue 2 , February 2003 , pp. 111 - 115
Copyright
2003 European Society of Anaesthesiology

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