Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-30T18:07:05.638Z Has data issue: false hasContentIssue false
  • English
  • Français

A modified radioimmunoassay for antibodies against Brucella abortus

Published online by Cambridge University Press:  25 March 2010

R. J. Chappel ,
J. Hayes ,
G. J. Brain  and
D. J. McNaught
R. J. Chappel
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
J. Hayes
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
G. J. Brain
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
D. J. McNaught
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The radioimmunoassay for brucellosis previously reported from this laboratory was a sensitive and useful method for detecting antibody against Brucella abortus in bovine serum. Changes in the procedure have improved sensitivity, have apparently increased interassay precision, and have made the assay easier to perform.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 1 , February 1982 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1982

References

Allan, G. S., Chappel, R. J., Williamson, P. & McNaught, D. J. (1976). A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes. Journal of Hygiene 76, 287.CrossRefGoogle ScholarPubMed
Alton, G. G., Jones, L. M. & Pietz, D. E. (1975). Laboratory techniques in brucellosis, 2nd ed.World Health Organization Monograph Series no. 55. Geneva.Google Scholar
Anon, (1977). Standardised complement fixation test for bovine brucellosis. Australian Veterinary Journal 53, 394.CrossRefGoogle Scholar
Chappel, R. J., Hayes, J., Rogerson, B. A. & Shenfield, L. J. (1981). The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests. Journal of Hygiene 88, 11.CrossRefGoogle Scholar
Chappel, R. J., McNaught, D. J., Bourke, J. A. & Allan, G. S. (1978 a). Comparison of the results of some serological tests for bovine brucellosis. Journal of Hygiene 80, 365.CrossRefGoogle ScholarPubMed
Chappel, R. J., McNaught, D. J., Bourke, J. A. & Allan, G. S. (1978 b). The diagnostic efficiency of some serological tests for bovine brucellosis. Journal of Hygiene 80, 373.CrossRefGoogle ScholarPubMed
Chappel, R. J., Williamson, P., McNaught, D. J., Dalling, M. J. & Allan, G. S. (1976). Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis. Journal of Hygiene 77, 369.CrossRefGoogle ScholarPubMed
Hayes, J. & Chappel, R. J. (1981). A comparison of the results of the brucellosis radioimmunoassay and other serological tests in experimentally infected cattle. Journal of Hygiene 88, 21.CrossRefGoogle Scholar
Marchalonis, J. J. (1969). An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal 113, 299.CrossRefGoogle ScholarPubMed
Woo, J. & Cannon, D. C. (1976). A quality control program for the radioimmunoassay laboratory. American Journal of Clinical Pathology 66, 854.CrossRefGoogle ScholarPubMed